公开(公告)号：US20130289264A1

公开(公告)日：2013-10-31

申请号：US13527103

申请日：2012-06-19

申请人： Chengyan ZHAO ,  Robert Ascenzi ,  Bijay K. Singh

发明人： Chengyan ZHAO ,  Robert Ascenzi ,  Bijay K. Singh

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/156

摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.

摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦

公开(公告)号：US08206906B2

公开(公告)日：2012-06-26

申请号：US12205246

申请日：2008-09-05

申请人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay K. Singh

发明人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay K. Singh

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/156

摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.

摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦

公开(公告)号：US20090098552A1

公开(公告)日：2009-04-16

申请号：US12205312

申请日：2008-09-05

申请人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay K. Singh

发明人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay K. Singh

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/156

摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.

摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦

公开(公告)号：US20090029381A1

公开(公告)日：2009-01-29

申请号：US12205246

申请日：2008-09-05

申请人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay K. Singh

发明人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay K. Singh

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/156

摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.

公开(公告)号：US07432082B2

公开(公告)日：2008-10-07

申请号：US10805973

申请日：2004-03-22

申请人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay K. Singh

发明人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay K. Singh

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02

CPC分类号： C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/156

摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.

摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦

公开(公告)号：US08877911B2

公开(公告)日：2014-11-04

申请号：US13527103

申请日：2012-06-19

申请人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay K. Singh

发明人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay K. Singh

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/156

摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.

摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦

公开(公告)号：US20120233723A1

公开(公告)日：2012-09-13

申请号：US13418018

申请日：2012-03-12

申请人： Alberto LIVORE ,  Alberto Raul Prina ,  Bijay K. Singh ,  Robert Ascenzi ,  Sherry R. Whitt

发明人： Alberto LIVORE ,  Alberto Raul Prina ,  Bijay K. Singh ,  Robert Ascenzi ,  Sherry R. Whitt

IPC分类号： C12N15/82 ,  C07K14/415 ,  A01H5/00 ,  A01P13/00 ,  A01C1/06 ,  C12N5/10 ,  A01N43/50 ,  C12N15/29 ,  A01H5/10

CPC分类号： C12N9/1022 ,  C12N9/88 ,  C12N15/8278

摘要： Herbicide-resistant rice plants, isolated polynucleotides that encode herbicide resistant and wild-type acetohydroxyacid synthase large subunit 1 (AHASL1) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to imidazolinone herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

摘要翻译： 描述除草剂抗性水稻植物，编码除草剂抗性和野生型乙酰羟基酸合成酶大亚基1（AHASL1）多肽的分离的多核苷酸，以及这些多肽的氨基酸序列。 描述了包含本发明多核苷酸的表达盒和转化载体，以及用多核苷酸转化的植物和宿主细胞。 还描述了使用多核苷酸增强植物对咪唑啉酮除草剂的抗性的方法，以及控制除草剂抗性植物附近杂草的方法。

公开(公告)号：US20100029485A1

公开(公告)日：2010-02-04

申请号：US11816884

申请日：2006-02-28

申请人： Alberto Livore ,  Alberto Raul Prina ,  Bijay K. Singh ,  Robert Ascenzi ,  Sherry R. Whitt

发明人： Alberto Livore ,  Alberto Raul Prina ,  Bijay K. Singh ,  Robert Ascenzi ,  Sherry R. Whitt

IPC分类号： A01N43/90 ,  A01H5/00 ,  A01H5/10 ,  A01N43/50 ,  A01N47/34 ,  A01N43/54 ,  A01N43/653 ,  C07H21/00 ,  C12N15/63 ,  C12N1/21 ,  C12N1/15 ,  C12N5/07 ,  C12N5/04 ,  A01P13/00 ,  A01H1/00 ,  C12Q1/02 ,  C12N9/00 ,  A01H1/02

CPC分类号： C12N9/1022 ,  C12N9/88 ,  C12N15/8278

摘要： Herbicide-resistant rice plants, isolated polynucleotides that encode herbicide resistant and wild-type acetohydroxy-acid synthase large subunit 1 (AHASL1) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to imidazolinone herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

摘要翻译： 描述了抗除草剂水稻植物，编码除草剂抗性和野生型乙酰羟基酸合成酶大亚基1（AHASL1）多肽的分离的多核苷酸，以及这些多肽的氨基酸序列。 描述了包含本发明多核苷酸的表达盒和转化载体，以及用多核苷酸转化的植物和宿主细胞。 还描述了使用多核苷酸增强植物对咪唑啉酮除草剂的抗性的方法，以及控制除草剂抗性植物附近杂草的方法。

公开(公告)号：US20050208506A1

公开(公告)日：2005-09-22

申请号：US10805973

申请日：2004-03-22

申请人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay Singh

发明人： Chengyan Zhao ,  Robert Ascenzi ,  Bijay Singh

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/156

摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.

摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦

公开(公告)号：US08563806B2

公开(公告)日：2013-10-22

申请号：US12522260

申请日：2008-02-04

申请人： Aaron Wiig ,  Robert Ascenzi ,  Xiang Huang ,  Sumita Chaudhuri ,  Rui-Guang Zhen ,  Yu Han

发明人： Aaron Wiig ,  Robert Ascenzi ,  Xiang Huang ,  Sumita Chaudhuri ,  Rui-Guang Zhen ,  Yu Han

IPC分类号： C12N15/82 ,  C12N15/87 ,  C12N15/63

CPC分类号： C07K14/415 ,  C12N15/8227 ,  C12N15/8239

摘要： The invention provides isolated promoter polynucleotides that are root-specific and/or induced by plant parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots and are particularly useful for controlling transcription of nucleic acids encoding agents that disrupt formation or maintenance of parasitic nematode feeding sites in plants.

摘要翻译： 本发明提供了由植物寄生线虫根特异性和/或诱导的分离的启动子多核苷酸。 本发明的启动子可用于控制植物根中感兴趣的核酸的表达，并且特别可用于控制编码破坏植物中寄生线虫饲养位点的形成或维持的试剂的转录。