公开(公告)号：US20090246146A1

公开(公告)日：2009-10-01

申请号：US12351487

申请日：2009-01-09

申请人： BOTOND BANFI ,  Anthony Fischer ,  Joseph Zabner ,  Lakshmi Durairaj ,  Daniel Lorentzen ,  Paul B. McCray, JR.

发明人： BOTOND BANFI ,  Anthony Fischer ,  Joseph Zabner ,  Lakshmi Durairaj ,  Daniel Lorentzen ,  Paul B. McCray, JR.

IPC分类号： A61K9/12 ,  A61K33/16 ,  A61K33/18 ,  A61K33/00 ,  A61P11/00

CPC分类号： A61K9/0078 ,  A61K33/00 ,  A61K33/16 ,  A61K33/18 ,  A61K45/06 ,  A61M15/009 ,  A61K2300/00

摘要： The present invention relates to the use of halides and halide salts for the treatment of microbial infections, including those caused by bacteria, fungi and viruses. The present invention takes advantage of endogenous immune function and augments this system using a non-toxic and inexpensive reagent that can be delivered to mucosal surfaces, for example, orally, topically, opthalmically and via inhalation.

摘要翻译： 本发明涉及卤化物和卤化物盐用于治疗微生物感染的用途，包括由细菌，真菌和病毒引起的感染。 本发明利用内源性免疫功能，并且使用可以递送至粘膜表面的无毒且便宜的试剂来增强该系统，例如口服，局部，眼部和通过吸入。

公开(公告)号：US08722639B2

公开(公告)日：2014-05-13

申请号：US13336147

申请日：2011-12-23

申请人： Karl-Heinz Krause ,  Botond Banfi

发明人： Karl-Heinz Krause ,  Botond Banfi

IPC分类号： C12N9/00 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11 ,  C07H21/04

CPC分类号： C12N15/1137 ,  A61K31/713 ,  A61K45/06 ,  A61K49/0008 ,  C12N2310/11 ,  C12N2320/31 ,  G01N33/5088 ,  G01N2333/90209 ,  G01N2800/14

摘要： This invention relates to modulators of NADPH oxidase as a medicament for the treatment and/or prevention of hearing loss and/or phantom hearing. Such modulators preferably act by inhibiting NADPH oxidase activity, wherein the NADPH oxidase comprises or consists of the amino acid sequence of any one of SEQ ID NO: 1, 3 or 5, or (ii) is encoded by a nucleic acid comprising or consisting of the sequence of any one of SEQ ID NO: 2, 4, 6, 23 or 24, or (iii) is a fragment of the protein according to (i) or (ii) and exhibits NADPH oxidase activity, or (iv) has a sequence at least 75% identical with the protein according to (i) or (ii) or with the fragment according to (iii) and exhibits NADPH oxidase activity. Also provided are pharmaceutical compositions, medical uses and diagnostic uses of compounds of the invention.

摘要翻译： 本发明涉及NADPH氧化酶的调节剂，其用作治疗和/或预防听力损失和/或幻影听觉的药物。 这种调节剂优选通过抑制NADPH氧化酶活性而起作用，其中NADPH氧化酶包含SEQ ID NO：1,3或5中任一个的氨基酸序列或由其组成，或（ii）由包含或由 SEQ ID NO：2,4,6,23或24中任一项的序列，或（iii）是根据（i）或（ii）的蛋白质的片段，并且表现出NADPH氧化酶活性，或（iv）具有 与根据（i）或（ii）的蛋白质或根据（iii）的片段至少75％相同的序列并显示NADPH氧化酶活性的序列。 还提供了本发明化合物的药物组合物，医疗用途和诊断用途。

公开(公告)号：US08088359B2

公开(公告)日：2012-01-03

申请号：US11628419

申请日：2005-06-06

申请人： Karl-Heinz Krause ,  Botond Banfi

发明人： Karl-Heinz Krause ,  Botond Banfi

IPC分类号： A61K49/00 ,  C12N9/00 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N15/1137 ,  A61K31/713 ,  A61K45/06 ,  A61K49/0008 ,  C12N2310/11 ,  C12N2320/31 ,  G01N33/5088 ,  G01N2333/90209 ,  G01N2800/14

摘要： This invention relates to a method of identifying a modulator of an NADPH oxidase, whereby said modulator is suitable as a lead compound and/or as a medicament for the treatment and/or prevention of hearing loss and/or phantom hearing, the method comprising the steps of (a) contacting a test compound with a protein, wherein said protein (i) comprises or consists of the amino acid sequence of any one of SEQ ID NO: 1, 3 or 5, or (ii) is encoded by a nucleic acid comprising or consisting of the sequence of any one of SEQ ID NO: 2, 4, 6, 23 or 24, or (iii) is a fragment of the protein according to (i) or (ii) and exhibits NADPH oxidase activity, or (iv) has a sequence at least 75% identical with the protein according to (i) or (ii) or with the fragment according to (iii) and exhibits NADPH oxidase activity, and optionally with one or more NADPH oxidase subunits, under conditions allowing binding of said test compound to said protein or, if present, said subunit(s); (b) optionally determining whether said test compound binds to said protein or, if present, said subunit(s); and (c) determining whether (ca) said test compound, upon contacting in step (a); or (cb) said test compound, upon binding in step (b) modulates the expression and/or activity of said protein or, if present, said subunit(s). Also provided are pharmaceutical compositions, medical uses and diagnostic uses of compounds of the invention.

摘要翻译： 本发明涉及一种鉴定NADPH氧化酶的调节剂的方法，其中所述调节剂适合作为用于治疗和/或预防听力损失和/或幻影听觉的铅化合物和/或药物，该方法包括 （a）使测试化合物与蛋白质接触的步骤，其中所述蛋白质（i）包含SEQ ID NO：1,3或5中任一个的氨基酸序列或由其组成，或（ii）由核酸编码 包含SEQ ID NO：2,4,6,23或24中任一项的序列或由其组成的酸，或（iii）是根据（i）或（ii）的蛋白质的片段，并且表现出NADPH氧化酶活性， 或（iv）具有与根据（i）或（ii）的蛋白质或根据（iii）的片段至少75％相同的序列并显示NADPH氧化酶活性，以及​​任选地含有一个或多个NADPH氧化酶亚基的序列， 允许所述测试化合物结合所述蛋白质或如果存在的话，所述亚基的条件; （b）任选地确定所述测试化合物是否结合所述蛋白质，或者如果存在，则是所述亚基; 和（c）在步骤（a）中确定（ca）所述测试化合物在接触时是否存在。 或（cb）所述测试化合物在步骤（b）中结合时调节所述蛋白质的表达和/或活性，或如果存在，则调节所述亚基。 还提供了本发明化合物的药物组合物，医疗用途和诊断用途。

公开(公告)号：US20120252868A1

公开(公告)日：2012-10-04

申请号：US13336147

申请日：2011-12-23

申请人： Karl-Heinz Krause ,  Botond Banfi

发明人： Karl-Heinz Krause ,  Botond Banfi

IPC分类号： A61K31/713 ,  A61P27/16

CPC分类号： C12N15/1137 ,  A61K31/713 ,  A61K45/06 ,  A61K49/0008 ,  C12N2310/11 ,  C12N2320/31 ,  G01N33/5088 ,  G01N2333/90209 ,  G01N2800/14

摘要： This invention relates to modulators of NADPH oxidase as a medicament for the treatment and/or prevention of hearing loss and/or phantom hearing. Such modulators preferably act by inhibiting NADPH oxidase activity, wherein the NADPH oxidase comprises or consists of the amino acid sequence of any one of SEQ ID NO: 1, 3 or 5, or (ii) is encoded by a nucleic acid comprising or consisting of the sequence of any one of SEQ ID NO: 2, 4, 6, 23 or 24, or (iii) is a fragment of the protein according to (i) or (ii) and exhibits NADPH oxidase activity, or (iv) has a sequence at least 75% identical with the protein according to (i) or (ii) or with the fragment according to (iii) and exhibits NADPH oxidase activity. Also provided are pharmaceutical compositions, medical uses and diagnostic uses of compounds of the invention.

摘要翻译： 本发明涉及NADPH氧化酶的调节剂，其用作治疗和/或预防听力损失和/或幻影听觉的药物。 这种调节剂优选通过抑制NADPH氧化酶活性而起作用，其中NADPH氧化酶包含SEQ ID NO：1,3或5中任一个的氨基酸序列或由其组成，或（ii）由包含或由 SEQ ID NO：2,4,6,23或24中任一项的序列，或（iii）是根据（i）或（ii）的蛋白质的片段，并且表现出NADPH氧化酶活性，或（iv）具有 与根据（i）或（ii）的蛋白质或根据（iii）的片段至少75％相同的序列并显示NADPH氧化酶活性的序列。 还提供了本发明化合物的药物组合物，医疗用途和诊断用途。

公开(公告)号：US20090263323A1

公开(公告)日：2009-10-22

申请号：US11628419

申请日：2005-06-06

申请人： Karl-Heinz Krause ,  Botond Banfi

发明人： Karl-Heinz Krause ,  Botond Banfi

IPC分类号： A61K49/00 ,  A61K39/395 ,  A61K38/44 ,  C12Q1/26 ,  C12N9/02 ,  C12N5/10 ,  C12N15/63 ,  A61K31/7088 ,  C07K16/00 ,  C07H21/04 ,  A01K67/00

CPC分类号： C12N15/1137 ,  A61K31/713 ,  A61K45/06 ,  A61K49/0008 ,  C12N2310/11 ,  C12N2320/31 ,  G01N33/5088 ,  G01N2333/90209 ,  G01N2800/14

摘要： This invention relates to a method of identifying a modulator of an NADPH oxidase, whereby said modulator is suitable as a lead compound and/or as a medicament for the treatment and/or prevention of hearing loss and/or phantom hearing, the method comprising the steps of (a) contacting a test compound with a protein, wherein said protein (i) comprises or consists of the amino acid sequence of any one of SEQ ID NO: 1, 3 or 5, or (ii) is encoded by a nucleic acid comprising or consisting of the sequence of any one of SEQ ID NO: 2, 4, 6, 23 or 24, or (iii) is a fragment of the protein according to (i) or (ii) and exhibits NADPH oxidase activity, or (iv) has a sequence at least 75% identical with the protein according to (i) or (ii) or with the fragment according to (iii) and exhibits NADPH oxidase activity, and optionally with one or more NADPH oxidase subunits, under conditions allowing binding of said test compound to said protein or, if present, said subunit(s); (b) optionally determining whether said test compound binds to said protein or, if present, said subunit(s); and (c) determining whether (ca) said test compound, upon contacting in step (a); or (cb) said test compound, upon binding in step (b) modulates the expression and/or activity of said protein or, if present, said subunit(s). Also provided are pharmaceutical compositions, medical uses and diagnostic uses of compounds of the invention.

摘要翻译： 本发明涉及一种鉴定NADPH氧化酶的调节剂的方法，其中所述调节剂适合作为用于治疗和/或预防听力损失和/或幻影听觉的铅化合物和/或药物，该方法包括 （a）使测试化合物与蛋白质接触的步骤，其中所述蛋白质（i）包含SEQ ID NO：1,3或5中任一个的氨基酸序列或由其组成，或（ii）由核酸编码 包含SEQ ID NO：2,4,6,23或24中任一项的序列或由其组成的酸，或（iii）是根据（i）或（ii）的蛋白质的片段，并且表现出NADPH氧化酶活性， 或（iv）具有与根据（i）或（ii）的蛋白质或根据（iii）的片段至少75％相同的序列并显示NADPH氧化酶活性，以及​​任选地含有一个或多个NADPH氧化酶亚基的序列， 允许所述测试化合物结合所述蛋白质或如果存在的话，所述亚基的条件; （b）任选地确定所述测试化合物是否结合所述蛋白质，或者如果存在，则是所述亚基; 和（c）在步骤（a）中确定（ca）所述测试化合物在接触时是否存在。 或（cb）所述测试化合物在步骤（b）中结合时调节所述蛋白质的表达和/或活性，或如果存在，则调节所述亚基。 还提供了本发明化合物的药物组合物，医疗用途和诊断用途。