公开(公告)号：US07605356B2

公开(公告)日：2009-10-20

申请号：US12172702

申请日：2008-07-14

申请人： Bruno Krief ,  Raphaël Marcelpoil

发明人： Bruno Krief ,  Raphaël Marcelpoil

IPC分类号： H01L27/00

CPC分类号： G02B21/244 ,  G06K9/00134 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A method of capturing a focused image of a continuously moving slide/objective arrangement is provided. A frame grabber device is triggered to capture an image of the slide through an objective at a first focus level as the slide continuously moves laterally relative to the objective. Alternatingly with triggering the frame grabber device, the objective is triggered to move to a second focus level after capture of the image of the slide. The objective moves in discrete steps, oscillating between minimum and maximum focus levels. The frame grabber device is triggered at a frequency as the slide continuously moves laterally relative to the objective so multiple images at different focus levels overlap, whereby a slide portion is common to each. The image having the maximum contrast value within overlapping images represents an optimum focus level for the slide portion, and thus the focused image. Associated apparatuses and methods are also provided.

摘要翻译： 提供了一种捕获连续移动的滑动/物镜布置的聚焦图像的方法。 触发帧抓取装置，当幻灯片相对于目标连续地横向移动时，以第一焦点级别通过物镜捕获幻灯片的图像。 交替地触发帧抓取器装置，在捕获幻灯片的图像之后触发目标移动到第二焦点水平。 目标以离散的步骤移动，在最小和最大对焦水平之间振荡。 当滑块相对于物镜横向移动时，帧抓取装置以频率被触发，因此在不同焦点级别的多个图像重叠，由此滑动部分是共同的。 在重叠图像中具有最大对比度值的图像表示滑动部分的最佳聚焦水平，并且因此表示聚焦图像。 还提供了相关的装置和方法。

公开(公告)号：US20080272273A1

公开(公告)日：2008-11-06

申请号：US12172636

申请日：2008-07-14

申请人： Bruno Krief ,  Raphael Marcelpoil

发明人： Bruno Krief ,  Raphael Marcelpoil

IPC分类号： G02B27/40

CPC分类号： G02B21/244 ,  G06K9/00134 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A method of capturing a focused image of a continuously moving slide/objective arrangement is provided. A frame grabber device is triggered to capture an image of the slide through an objective at a first focus level as the slide continuously moves laterally relative to the objective. Alternatingly with triggering the frame grabber device, the objective is triggered to move to a second focus level after capture of the image of the slide. The objective moves in discrete steps, oscillating between minimum and maximum focus levels. The frame grabber device is triggered at a frequency as the slide continuously moves laterally relative to the objective so multiple images at different focus levels overlap, whereby a slide portion is common to each. The image having the maximum contrast value within overlapping images represents an optimum focus level for the slide portion, and thus the focused image. Associated apparatuses and methods are also provided.

摘要翻译： 提供了一种捕获连续移动的滑动/物镜布置的聚焦图像的方法。 触发帧抓取装置，当幻灯片相对于目标连续地横向移动时，以第一焦点级别通过物镜捕获幻灯片的图像。 交替地触发帧抓取器装置，在捕获幻灯片的图像之后触发目标移动到第二焦点水平。 目标以离散的步骤移动，在最小和最大对焦水平之间振荡。 当滑块相对于物镜横向移动时，帧抓取装置以频率被触发，因此在不同焦点级别的多个图像重叠，由此滑动部分是共同的。 在重叠图像中具有最大对比度值的图像表示滑动部分的最佳聚焦水平，并且因此表示聚焦图像。 还提供了相关的装置和方法。

公开(公告)号：US07772535B2

公开(公告)日：2010-08-10

申请号：US12172723

申请日：2008-07-14

申请人： Bruno Krief ,  Raphaël Marcelpoil

发明人： Bruno Krief ,  Raphaël Marcelpoil

IPC分类号： H01L27/00

CPC分类号： G02B21/244 ,  G06K9/00134 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A method of capturing a focused image of a continuously moving slide/objective arrangement is provided. A frame grabber device is triggered to capture an image of the slide through an objective at a first focus level as the slide continuously moves laterally relative to the objective. Alternatingly with triggering the frame grabber device, the objective is triggered to move to a second focus level after capture of the image of the slide. The objective moves in discrete steps, oscillating between minimum and maximum focus levels. The frame grabber device is triggered at a frequency as the slide continuously moves laterally relative to the objective so multiple images at different focus levels overlap, whereby a slide portion is common to each. The image having the maximum contrast value within overlapping images represents an optimum focus level for the slide portion, and thus the focused image. Associated apparatuses and methods are also provided.

摘要翻译： 提供了一种捕获连续移动的滑动/物镜布置的聚焦图像的方法。 触发帧抓取装置，当幻灯片相对于目标连续地横向移动时，以第一焦点级别通过物镜捕获幻灯片的图像。 交替地触发帧抓取器装置，在捕获幻灯片的图像之后触发目标移动到第二焦点水平。 目标以离散的步骤移动，在最小和最大对焦水平之间振荡。 当滑块相对于物镜横向移动时，帧抓取装置以频率被触发，因此在不同焦点级别的多个图像重叠，由此滑动部分是共同的。 在重叠图像中具有最大对比度值的图像表示滑动部分的最佳聚焦水平，并且因此表示聚焦图像。 还提供了相关的装置和方法。

公开(公告)号：US20080272274A1

公开(公告)日：2008-11-06

申请号：US12172723

申请日：2008-07-14

申请人： Bruno Krief ,  Raphael Marcelpoil

发明人： Bruno Krief ,  Raphael Marcelpoil

IPC分类号： G02B27/40

CPC分类号： G02B21/244 ,  G06K9/00134 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A method of capturing a focused image of a continuously moving slide/objective arrangement is provided. A frame grabber device is triggered to capture an image of the slide through an objective at a first focus level as the slide continuously moves laterally relative to the objective. Alternatingly with triggering the frame grabber device, the objective is triggered to move to a second focus level after capture of the image of the slide. The objective moves in discrete steps, oscillating between minimum and maximum focus levels. The frame grabber device is triggered at a frequency as the slide continuously moves laterally relative to the objective so multiple images at different focus levels overlap, whereby a slide portion is common to each. The image having the maximum contrast value within overlapping images represents an optimum focus level for the slide portion, and thus the focused image. Associated apparatuses and methods are also provided.

摘要翻译： 提供了一种捕获连续移动的滑动/物镜布置的聚焦图像的方法。 触发帧抓取装置，当幻灯片相对于目标连续地横向移动时，以第一焦点级别通过物镜捕获幻灯片的图像。 交替地触发帧抓取器装置，在捕获幻灯片的图像之后触发目标移动到第二焦点水平。 目标以离散的步骤移动，在最小和最大对焦水平之间振荡。 当滑块相对于物镜横向移动时，帧抓取装置以频率被触发，因此在不同焦点级别的多个图像重叠，由此滑动部分是共同的。 在重叠图像中具有最大对比度值的图像表示滑动部分的最佳聚焦水平，并且因此表示聚焦图像。 还提供了相关的装置和方法。

公开(公告)号：US20080273776A1

公开(公告)日：2008-11-06

申请号：US12172702

申请日：2008-07-14

申请人： Bruno Krief ,  Raphael Marcelpoil

发明人： Bruno Krief ,  Raphael Marcelpoil

IPC分类号： G06K9/00

CPC分类号： G02B21/244 ,  G06K9/00134 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A method of capturing a focused image of a continuously moving slide/objective arrangement is provided. A frame grabber device is triggered to capture an image of the slide through an objective at a first focus level as the slide continuously moves laterally relative to the objective. Alternatingly with triggering the frame grabber device, the objective is triggered to move to a second focus level after capture of the image of the slide. The objective moves in discrete steps, oscillating between minimum and maximum focus levels. The frame grabber device is triggered at a frequency as the slide continuously moves laterally relative to the objective so multiple images at different focus levels overlap, whereby a slide portion is common to each. The image having the maximum contrast value within overlapping images represents an optimum focus level for the slide portion, and thus the focused image. Associated apparatuses and methods are also provided.

公开(公告)号：US07417213B2

公开(公告)日：2008-08-26

申请号：US11425605

申请日：2006-06-21

申请人： Bruno Krief ,  Raphaël Marcelpoil

发明人： Bruno Krief ,  Raphaël Marcelpoil

IPC分类号： H01L27/00

CPC分类号： G02B21/244 ,  G06K9/00134 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A method of capturing a focused image of a continuously moving slide/objective arrangement is provided. A frame grabber device is triggered to capture an image of the slide through an objective at a first focus level as the slide continuously moves laterally relative to the objective. Alternatingly with triggering the frame grabber device, the objective is triggered to move to a second focus level after capture of the image of the slide. The objective moves in discrete steps, oscillating between minimum and maximum focus levels. The frame grabber device is triggered at a frequency as the slide continuously moves laterally relative to the objective so multiple images at different focus levels overlap, whereby a slide portion is common to each. The image having the maximum contrast value within overlapping images represents an optimum focus level for the slide portion, and thus the focused image. Associated apparatuses and methods are also provided.

摘要翻译： 提供了一种捕获连续移动的滑动/物镜布置的聚焦图像的方法。 触发帧抓取装置，当幻灯片相对于目标连续地横向移动时，以第一焦点级别通过物镜捕获幻灯片的图像。 交替地触发帧抓取器装置，在捕获幻灯片的图像之后触发目标移动到第二焦点水平。 目标以离散的步骤移动，在最小和最大对焦水平之间振荡。 当滑块相对于物镜横向移动时，帧抓取装置以频率被触发，因此在不同焦点级别的多个图像重叠，由此滑动部分是共同的。 在重叠图像中具有最大对比度值的图像表示滑动部分的最佳聚焦水平，并且因此表示聚焦图像。 还提供了相关的装置和方法。

公开(公告)号：US20070069106A1

公开(公告)日：2007-03-29

申请号：US11425605

申请日：2006-06-21

申请人： Bruno Krief ,  Raphael Marcelpoil

发明人： Bruno Krief ,  Raphael Marcelpoil

IPC分类号： G02B27/40 ,  G02B27/64

CPC分类号： G02B21/244 ,  G06K9/00134 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A method of capturing a focused image of a continuously moving slide/objective arrangement is provided. A frame grabber device is triggered to capture an image of the slide through an objective at a first focus level as the slide continuously moves laterally relative to the objective. Alternatingly with triggering the frame grabber device, the objective is triggered to move to a second focus level after capture of the image of the slide. The objective moves in discrete steps, oscillating between minimum and maximum focus levels. The frame grabber device is triggered at a frequency as the slide continuously moves laterally relative to the objective so multiple images at different focus levels overlap, whereby a slide portion is common to each. The image having the maximum contrast value within overlapping images represents an optimum focus level for the slide portion, and thus the focused image. Associated apparatuses and methods are also provided.

摘要翻译： 提供了一种捕获连续移动的滑动/物镜布置的聚焦图像的方法。 触发帧抓取装置，当幻灯片相对于目标连续地横向移动时，以第一焦点级别通过物镜捕获幻灯片的图像。 交替地触发帧抓取器装置，在捕获幻灯片的图像之后触发目标移动到第二焦点水平。 目标以离散的步骤移动，在最小和最大对焦水平之间振荡。 当滑块相对于物镜横向移动时，帧抓取装置以频率被触发，因此在不同焦点级别的多个图像重叠，由此滑动部分是共同的。 在重叠图像中具有最大对比度值的图像表示滑动部分的最佳聚焦水平，并且因此表示聚焦图像。 还提供了相关的装置和方法。

公开(公告)号：US07638748B2

公开(公告)日：2009-12-29

申请号：US12172636

申请日：2008-07-14

申请人： Bruno Krief ,  Raphaël Marcelpoil

发明人： Bruno Krief ,  Raphaël Marcelpoil

IPC分类号： H01L27/00

CPC分类号： G02B21/244 ,  G06K9/00134 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A method of capturing a focused image of a continuously moving slide/objective arrangement is provided. A frame grabber device is triggered to capture an image of the slide through an objective at a first focus level as the slide continuously moves laterally relative to the objective. Alternatingly with triggering the frame grabber device, the objective is triggered to move to a second focus level after capture of the image of the slide. The objective moves in discrete steps, oscillating between minimum and maximum focus levels. The frame grabber device is triggered at a frequency as the slide continuously moves laterally relative to the objective so multiple images at different focus levels overlap, whereby a slide portion is common to each. The image having the maximum contrast value within overlapping images represents an optimum focus level for the slide portion, and thus the focused image. Associated apparatuses and methods are also provided.

摘要翻译： 提供了一种捕获连续移动的滑动/物镜布置的聚焦图像的方法。 触发帧抓取装置，当幻灯片相对于目标连续地横向移动时，以第一焦点级别通过物镜捕获幻灯片的图像。 交替地触发帧抓取器装置，在捕获幻灯片的图像之后触发目标移动到第二焦点水平。 目标以离散的步骤移动，在最小和最大对焦水平之间振荡。 当滑块相对于物镜横向移动时，帧抓取装置以频率被触发，因此在不同焦点级别的多个图像重叠，由此滑动部分是共同的。 在重叠图像中具有最大对比度值的图像表示滑动部分的最佳聚焦水平，并且因此表示聚焦图像。 还提供了相关的装置和方法。

公开(公告)号：US20090196526A1

公开(公告)日：2009-08-06

申请号：US12415015

申请日：2009-03-31

申请人： Joachim Helmut Schmid ,  Thomas Gahm ,  Kevin Russell Kooy ,  Rainer Dorrer ,  Bruno Krief ,  John Royal Cheeseman

发明人： Joachim Helmut Schmid ,  Thomas Gahm ,  Kevin Russell Kooy ,  Rainer Dorrer ,  Bruno Krief ,  John Royal Cheeseman

IPC分类号： G06K9/64 ,  G06K9/36

CPC分类号： G02B21/0016 ,  G01N15/042 ,  G01N15/05 ,  G01N2035/00039 ,  G02B21/26 ,  G02B21/367 ,  G06K9/00127 ,  G06T2200/32

摘要： A scanning device for biological slides is provided, which can be operated in an interactive routine mode as well as in an unsupervised high speed automatic mode. In the first case, typical components, which the pathologist is used to operating manually, such as the microscope, the stage and the focus, and which have to be motorized for the automatic unsupervised system mode, are configured to simulate manual use, operation, and response. A non-interlaced area scan camera supports the interactive selection and acquisition of individual images in the manual mode, as well as the continuous high-speed scan motion for the rare event detection and virtual slide scan applications of the system. Due to the particular requirements to accommodate both operational modes, methods are described for constructing the virtual slide out of image tiles with varying overlap areas in the x- and y-directions.

摘要翻译： 提供了用于生物幻灯片的扫描装置，其可以以交互式例程模式以及无监督的高速自动模式操作。 在第一种情况下，病理学家用于手动操作的典型组件（如显微镜，平台和焦点）以及必须为自动无人监控系统模式而进行电动操作，被配置为模拟手动使用，操作， 和回应。 非隔行扫描摄像机支持在手动模式下交互式选择和采集各图像，以及用于系统的罕见事件检测和虚拟幻灯片扫描应用的连续高速扫描运动。 由于适应两种操作模式的特殊要求，描述了用于构造在x和y方向上具有不同重叠区域的图像瓦片中的虚拟幻灯片的方法。

公开(公告)号：US20060133657A1

公开(公告)日：2006-06-22

申请号：US11204954

申请日：2005-08-16

申请人： Joachim Schmid ,  Thomas Gahm ,  Kevin Kooy ,  Rainer Dorrer ,  Bruno Krief ,  John Cheeseman

发明人： Joachim Schmid ,  Thomas Gahm ,  Kevin Kooy ,  Rainer Dorrer ,  Bruno Krief ,  John Cheeseman

IPC分类号： G06K9/00

CPC分类号： G02B21/0016 ,  G01N15/042 ,  G01N15/05 ,  G01N2035/00039 ,  G02B21/26 ,  G02B21/367 ,  G06K9/00127 ,  G06T2200/32

摘要： A scanning device for biological slides is provided, which can be operated in an interactive routine mode as well as in an unsupervised high speed automatic mode. In the first case, typical components, which the pathologist is used to operating manually, such as the microscope, the stage and the focus, and which have to be motorized for the automatic unsupervised system mode, are configured to simulate manual use, operation, and response. A non-interlaced area scan camera supports the interactive selection and acquisition of individual images in the manual mode, as well as the continuous high-speed scan motion for the rare event detection and virtual slide scan applications of the system. Due to the particular requirements to accommodate both operational modes, methods are described for constructing the virtual slide out of image tiles with varying overlap areas in the x- and y-directions.

摘要翻译： 提供了用于生物幻灯片的扫描装置，其可以以交互式例程模式以及无监督的高速自动模式操作。 在第一种情况下，病理学家用于手动操作的典型组件（如显微镜，平台和焦点）以及必须为自动无人监控系统模式而进行电动操作，被配置为模拟手动使用，操作， 和回应。 非隔行扫描摄像机支持在手动模式下交互式选择和采集各图像，以及用于系统的罕见事件检测和虚拟幻灯片扫描应用的连续高速扫描运动。 由于适应两种操作模式的特殊要求，描述了用于构造在x和y方向上具有不同重叠区域的图像瓦片中的虚拟幻灯片的方法。