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    Plant resistance to insect pests mediated by viral proteins
    1.
    发明授权
    Plant resistance to insect pests mediated by viral proteins 失效
    植物对病毒蛋白介导的害虫的抗性

    公开(公告)号：US07312080B2

    公开(公告)日：2007-12-25

    申请号：US10346144

    申请日：2003-01-15

    申请人： W. Allen Miller ,  Bryony C. Bonning

    发明人： W. Allen Miller ,  Bryony C. Bonning

    IPC分类号： C12N5/00 ,  C12N15/63 ,  C12P21/06 ,  A61K38/00 ,  C07H21/04

    CPC分类号： C07K14/005 ,  A01N63/02 ,  C07K14/43522 ,  C07K2319/00 ,  C12N15/8286 ,  C12N2770/00022 ,  Y02A40/162

    摘要： The invention involves combining a peptide toxin effective against insects, including but not limited to thrips, leaf hoppers, and beetles, with a transport peptide capable of facilitating transfer of the peptide toxin from the gut of an insect to the hemocoel. The combination can be effected by a fusion of genetic material encoding the peptide toxin and the transport peptide, such that expression of the genetic material fusion results in synthesis of a fusion protein combining the functions of both the toxin and the transport protein. Ingestion of the fusion protein by the sucking insect transfers the fusion protein into the insect's gut from which it is transferred into the hemocoel due to the functional activity of the transport peptide where the toxin exerts its toxic effect upon the insect. In a preferred embodiment, the invention is effective in control of such sucking insects as aphids, whiteflies and the like, and other vectors that transmit viruses in a circulative manner.

    摘要翻译： 本发明涉及将能够有效地抵抗昆虫（包括但不限于蓟马，叶子料斗和甲虫）的肽毒素与能够促进肽毒素从昆虫的肠道转移到血液凝块的转运肽组合。 该组合可以通过编码肽毒素和转运肽的遗传物质的融合来实现，使得遗传物质融合的表达导致融合蛋白质的合成并结合毒素和转运蛋白的功能。 通过吸吮昆虫摄入融合蛋白，由于毒素对昆虫发挥其毒性作用的运输肽的功能活性，将融合蛋白转移到昆虫的肠中，由此将其转移到血液凝块中。 在优选的实施方式中，本发明能有效地控制蚜虫，粉虱等吸吮昆虫，以及以循环方式传播病毒的其它载体。

    Basement membrane degrading proteases as insect toxins and methods of use for same
    2.
    发明授权
    Basement membrane degrading proteases as insect toxins and methods of use for same 失效
    地下室降解蛋白酶作为昆虫毒素及其使用方法

    公开(公告)号：US06673340B1

    公开(公告)日：2004-01-06

    申请号：US09614789

    申请日：2000-07-12

    申请人： Robert L. Harrison ,  Bryony C. Bonning

    发明人： Robert L. Harrison ,  Bryony C. Bonning

    IPC分类号： A01N6300

    CPC分类号： C12N9/6472 ,  A01N63/00 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

    摘要： Novel insect toxins are disclosed for use in pesticidal compositions and methods. According to the invention basement membrane degrading proteases are identified which are capable of acting as insecticidal agents. Polynucleotides are provided which include expression constructs for the expression of the recombinant insecticidal proteases of the invention as recombinant insect pathogens, as well as transgenic plants with a substantial degree of insect resistance.

    摘要翻译： 公开了新型昆虫毒素用于农药组合物和方法。 根据本发明，鉴定出可以作为杀虫剂的基底膜降解蛋白酶。 提供了多核苷酸，其包括用于表达本发明的重组杀虫蛋白酶作为重组昆虫病原体的表达构建体，以及具有相当程度的抗虫性的转基因植物。