公开(公告)号：US07312080B2

公开(公告)日：2007-12-25

申请号：US10346144

申请日：2003-01-15

申请人： W. Allen Miller ,  Bryony C. Bonning

发明人： W. Allen Miller ,  Bryony C. Bonning

IPC分类号： C12N5/00 ,  C12N15/63 ,  C12P21/06 ,  A61K38/00 ,  C07H21/04

CPC分类号： C07K14/005 ,  A01N63/02 ,  C07K14/43522 ,  C07K2319/00 ,  C12N15/8286 ,  C12N2770/00022 ,  Y02A40/162

摘要： The invention involves combining a peptide toxin effective against insects, including but not limited to thrips, leaf hoppers, and beetles, with a transport peptide capable of facilitating transfer of the peptide toxin from the gut of an insect to the hemocoel. The combination can be effected by a fusion of genetic material encoding the peptide toxin and the transport peptide, such that expression of the genetic material fusion results in synthesis of a fusion protein combining the functions of both the toxin and the transport protein. Ingestion of the fusion protein by the sucking insect transfers the fusion protein into the insect's gut from which it is transferred into the hemocoel due to the functional activity of the transport peptide where the toxin exerts its toxic effect upon the insect. In a preferred embodiment, the invention is effective in control of such sucking insects as aphids, whiteflies and the like, and other vectors that transmit viruses in a circulative manner.

摘要翻译： 本发明涉及将能够有效地抵抗昆虫（包括但不限于蓟马，叶子料斗和甲虫）的肽毒素与能够促进肽毒素从昆虫的肠道转移到血液凝块的转运肽组合。 该组合可以通过编码肽毒素和转运肽的遗传物质的融合来实现，使得遗传物质融合的表达导致融合蛋白质的合成并结合毒素和转运蛋白的功能。 通过吸吮昆虫摄入融合蛋白，由于毒素对昆虫发挥其毒性作用的运输肽的功能活性，将融合蛋白转移到昆虫的肠中，由此将其转移到血液凝块中。 在优选的实施方式中，本发明能有效地控制蚜虫，粉虱等吸吮昆虫，以及以循环方式传播病毒的其它载体。

公开(公告)号：US20090055975A1

公开(公告)日：2009-02-26

申请号：US12001776

申请日：2007-12-11

申请人： Bryony Bonning ,  W. Allen Miller ,  Sijun Liu

发明人： Bryony Bonning ,  W. Allen Miller ,  Sijun Liu

IPC分类号： C07K7/08 ,  C12N15/11 ,  C12N15/62 ,  A01H5/00 ,  A61P31/12

CPC分类号： C07K7/08 ,  C07K14/005 ,  C12N15/8283 ,  C12N2770/00022

摘要： Provided is a peptide, peptide multimer or fusion protein containing such peptide which binds to the gut of sap-sucking insects, e.g., aphids. When bound, this peptide inhibits the binding of targeted virus to the insect gut. When this peptide or tandem repeat peptide is expressed (or is expressed as part of a fusion protein) in the fluids of transgenic plants, it is taken up by the insect with the sap, binds to gut receptors and thereby inhibits transmission of the virus from plant to plant via the insect vector. Thus, the use of such transgenic plants blocks or reduces the transmission of the targeted virus and others which share the peptide binding site within the insect gut among plants susceptible to the virus and thereby reduces losses due to viral infection.

摘要翻译： 提供了含有这种肽的肽，肽多聚体或融合蛋白，其结合吸吮昆虫的肠道，例如蚜虫。 当肽结合时，该肽抑制靶向病毒与昆虫肠道的结合。 当这种肽或串联重复肽在转基因植物的液体中表达（或作为融合蛋白的一部分表达）时，它被昆虫与树汁吸收，结合肠道受体，从而抑制病毒从 植物通过昆虫载体植物。 因此，这种转基因植物的使用阻止或减少在易感病毒的植物中在昆虫肠内共享肽结合位点的目标病毒和其他物质的传播，从而减少由于病毒感染引起的损失。

公开(公告)号：US5910628A

公开(公告)日：1999-06-08

申请号：US858623

申请日：1997-05-20

申请人： W. Allen Miller ,  Shanping Wang

发明人： W. Allen Miller ,  Shanping Wang

IPC分类号： C12N15/67 ,  C12N15/81 ,  C12N15/82 ,  C12N15/83 ,  A01H1/04

CPC分类号： C12N15/67 ,  C12N15/81 ,  C12N15/8216

摘要： The present invention provides a method of increasing the production of a protein translated from an uncapped eukaryotic messenger ribonucleic acid (mRNA), comprising the steps of: selecting a nucleotide sequence encoding a protein to be expressed; joining nucleotides 1-168 of the barley yellow dwarf virus RNA, PAV serotype to the 5' untranslated region of said uncapped mRNA, said nucleotides 1-168 comprising the 5' untranslated region plus first 27 nucleotides of the open reading frame of the barley yellow dwarf virus RNA; linking nucleotides 4513-5677 of the barley yellow dwarf virus RNA, PAV serotype, to the 3' untranslated region of said uncapped mRNA; and expressing the protein. Also provided is a DNA molecule which comprises: (a) a promoter region which functions in plant cells to cause the production of an RNA sequence, which is operably linked to (b) a 5' untranslated region including a 5' translation enhancing segment, said 5' translation enhancing segment comprising nucleotides 1-168 of the barley yellow dwarf virus RNA, PAV serotype, said nucleotides 1-168 encompassing the 5' untranslated region plus first 27 nucleotides of the open reading frame of the barley yellow dwarf virus RNA; (c) a coding sequence, wherein said coding sequence is heterologous to said 5' untranslated region, which is operably linked to (d) a 3' untranslated region that functions in plant cells to cause the termination of transcription, said 3' untranslated region comprising nucleotides 4513-5677 of the barley yellow dwarf virus RNA.

摘要翻译： 本发明提供了一种增加从未加帽的真核信使核糖核酸（mRNA）翻译的蛋白质的产生的方法，包括以下步骤：选择编码待表达蛋白质的核苷酸序列; 将大麦黄矮病毒RNA，PAV血清型的核苷酸1-168连接到所述未加帽的mRNA的5'非翻译区，所述核苷酸1-168包含大麦黄开放阅读框的5'非翻译区加上第27个核苷酸 矮病毒RNA; 将大麦黄矮病毒RNA（PAV血清型）的核苷酸4513-5677连接到所述未加帽的mRNA的3'非翻译区; 并表达蛋白质。 还提供了DNA分子，其包含：（a）在植物细胞中起作用以产生RNA序列的启动子区，其可操作地连接到（b）包含5'翻译增强区的5'非翻译区， 所述5'翻译增强区段包含大麦黄矮病毒RNA（PAV血清型）的核苷酸1-168，所述核苷酸1-168包含大麦黄矮病毒RNA的开放阅读框的5'非翻译区加上第27个核苷酸; （c）编码序列，其中所述编码序列与所述5'非翻译区异源，其可操作地连接到（d）在植物细胞中起作用以引起转录终止的3'非翻译区，所述3'非翻译区 包括大麦黄矮病毒RNA的核苷酸4513-5677。

公开(公告)号：US07547677B2

公开(公告)日：2009-06-16

申请号：US12001776

申请日：2007-12-11

申请人： Bryony Bonning ,  W. Allen Miller ,  Sijun Liu

发明人： Bryony Bonning ,  W. Allen Miller ,  Sijun Liu

IPC分类号： A61K38/10 ,  C07K7/08 ,  C12P21/06 ,  A01H1/00 ,  C12N15/11

CPC分类号： C07K7/08 ,  C07K14/005 ,  C12N15/8283 ,  C12N2770/00022

摘要： Provided is a peptide, peptide multimer or fusion protein containing such peptide which binds to the gut of sap-sucking insects, e.g., aphids. When bound, this peptide inhibits the binding of targeted virus to the insect gut. When this peptide or tandem repeat peptide is expressed (or is expressed as part of a fusion protein) in the fluids of transgenic plants, it is taken up by the insect with the sap, binds to gut receptors and thereby inhibits transmission of the virus from plant to plant via the insect vector. Thus, the use of such transgenic plants blocks or reduces the transmission of the targeted virus and others which share the peptide binding site within the insect gut among plants susceptible to the virus and thereby reduces losses due to viral infection.