公开(公告)号：US07745177B2

公开(公告)日：2010-06-29

申请号：US11677685

申请日：2007-02-22

申请人： Jung-Hoe Kim ,  Byoung-Sam Ko

发明人： Jung-Hoe Kim ,  Byoung-Sam Ko

IPC分类号： C12P19/00 ,  C12N9/00 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12P7/18

摘要： The present invention provides a method for manufacturing xylitol with high-yield and high-productivity by using a xylitol dehydrogenase-deficient mutant of xylitol producing microorganism. This goal is achieved through modification of the metabolic pathway of the xylitol producing microorganism, preferably a natural xylose-assimilating yeasts and fungi, by disrupting or inactivating the expression of desired genes.

摘要翻译： 本发明提供了通过使用木糖醇生产微生物的木糖醇脱氢酶缺陷型突变体以高产率和高生产率制备木糖醇的方法。 通过破坏或失活所需基因的表达，通过修饰木糖醇生产微生物，优选天然木糖同化的酵母和真菌的代谢途径来实现这一目标。

公开(公告)号：US20080206821A1

公开(公告)日：2008-08-28

申请号：US11677685

申请日：2007-02-22

申请人： Jung-Hoe Kim ,  Byoung-Sam Ko

发明人： Jung-Hoe Kim ,  Byoung-Sam Ko

IPC分类号： C12P19/00

CPC分类号： C12P7/18

摘要： The present invention provides a method for manufacturing xylitol with high-yield and high-productivity by using a xylitol dehydrogenase-deficient mutant of xylitol producing microorganism. This goal is achieved through modification of the metabolic pathway of the xylitol producing microorganism, preferably a natural xylose-assimilating yeasts and fungi, by disrupting or inactivating the expression of desired genes.

摘要翻译： 本发明提供了通过使用木糖醇生产微生物的木糖醇脱氢酶缺陷型突变体以高产率和高生产率制备木糖醇的方法。 通过破坏或失活所需基因的表达，通过修饰木糖醇生产微生物，优选天然木糖同化的酵母和真菌的代谢途径来实现这一目标。

公开(公告)号：US07820414B2

公开(公告)日：2010-10-26

申请号：US11771081

申请日：2007-06-29

申请人： Jung Hoe Kim ,  Byoung Sam Ko

发明人： Jung Hoe Kim ,  Byoung Sam Ko

IPC分类号： C12P7/18 ,  C12P19/02 ,  C12N1/10

CPC分类号： C12N1/16 ,  C12P7/18

摘要： Disclosed herein are Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of Candida tropicalis, a method of producing a high yield of xylitol using the same, and xylitol produced by the method. More specifically, disclosed are a method for producing a high yield of xylitol, in which a high concentration of xylose contained in a biomass hydrolyzate is converted to xylitol using xylitol dehydrogenase-inactivated mutant of Candida tropicalis, without controlling dissolved oxygen to a low level, as well as xylitol produced according to the method. Also disclosed are a xylitol production method, in which the production of byproduct arabitol, which is produced when using a biomass as a substrate and adversely affects the yield of xylitol, is significantly reduced through the use of Candida tropicalis mutant ara-89 (KCTC 11136bp) having an inhibited activity of arabinose reductase converting arabinose to arabitol, thus increasing xylitol productivity, as well as xylitol produced by the method.

摘要翻译： 本文公开了热带假丝酵母的木糖醇脱氢酶失活和阿拉伯糖还原酶抑制突变体，使用其产生高产率的木糖醇的方法，以及通过该方法生产的木糖醇。 更具体地，公开了一种生产高产率的木糖醇的方法，其中将生物质水解产物中所含的高浓度的木糖转化为木糖醇，使用热带假丝酵母的木糖醇脱氢酶灭活的突变体，而不将溶解氧控制在低水平， 以及根据该方法生产的木糖醇。 还公开了一种木糖醇生产方法，其中通过使用热带假丝酵母突变体ara-89（KCTC 11136bp）显着降低在使用生物质作为底物时产生的副产物阿拉伯糖醇的产生并且不利地影响木糖醇的产率 ）具有抑制阿拉伯糖还原酶的活性，其将阿拉伯糖转化成阿糖醇，从而增加木糖醇生产率，以及通过该方法产生的木糖醇。

公开(公告)号：US20090004704A1

公开(公告)日：2009-01-01

申请号：US11771081

申请日：2007-06-29

申请人： Jung Hoe Kim ,  Byoung Sam Ko

发明人： Jung Hoe Kim ,  Byoung Sam Ko

IPC分类号： C12P19/02 ,  C07H1/00 ,  C12N1/20

CPC分类号： C12N1/16 ,  C12P7/18

摘要： Disclosed herein are Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of Candida tropicalis, a method of producing a high yield of xylitol using the same, and xylitol produced by the method. More specifically, disclosed are a method for producing a high yield of xylitol, in which a high concentration of xylose contained in a biomass hydrolyzate is converted to xylitol using xylitol dehydrogenase-inactivated mutant of Candida tropicalis, without controlling dissolved oxygen to a low level, as well as xylitol produced according to the method. Also disclosed are a xylitol production method, in which the production of byproduct arabitol, which is produced when using a biomass as a substrate and adversely affects the yield of xylitol, is significantly reduced through the use of Candida tropicalis mutant ara-89 (KCTC 11136bp) having an inhibited activity of arabinose reductase converting arabinose to arabitol, thus increasing xylitol productivity, as well as xylitol produced by the method.

摘要翻译： 本文公开了热带假丝酵母的木糖醇脱氢酶失活和阿拉伯糖还原酶抑制突变体，使用其产生高产率的木糖醇的方法，以及通过该方法生产的木糖醇。 更具体地，公开了一种生产高产率的木糖醇的方法，其中将生物质水解产物中所含的高浓度的木糖转化为木糖醇，使用热带假丝酵母的木糖醇脱氢酶灭活的突变体，而不将溶解氧控制在低水平， 以及根据该方法生产的木糖醇。 还公开了一种木糖醇生产方法，其中通过使用热带假丝酵母突变体ara-89（KCTC 11136bp）显着降低在使用生物质作为底物时产生的副产物阿拉伯糖醇的产生并且不利地影响木糖醇的产率 ）具有抑制阿拉伯糖还原酶的活性，其将阿拉伯糖转化成阿糖醇，从而增加木糖醇生产率，以及通过该方法产生的木糖醇。