STRAIN FOR PRODUCING LONG-CHAIN DICARBOXYLIC ACIDS AND FERMENTATION METHOD THEREFOR

    公开(公告)号:US20230203431A1

    公开(公告)日:2023-06-29

    申请号:US17769459

    申请日:2020-01-16

    IPC分类号: C12N1/16 C12P7/44

    摘要: Provided are a Candida tropicalis strain Am2525, with the preservation number thereof being CCTCC NO: M 2019419, and a method for producing long-chain dicarboxylic acids by means of fermenting the strain. The method for producing the long-chain dicarboxylic acids comprises preparing a seed solution by means of the Candida tropicalis strain Am2525 and producing the long-chain dicarboxylic acids via fermentation of the seed solution. Compared with the parent, the Candida tropicalis strain Am2525 has an enhanced resistance to the toxicity of a substrate decane, improves the productivity of long-chain dicarboxylic acids, reduces the cost of production, subsequent separation and purification are simple, and the fermentation production process is easy to implement on a large scale.

    Method for producing a long chain dicarboxylic acid by fermentation, fermentation broth, treated fermentation broth and wastewater

    公开(公告)号:US11319561B2

    公开(公告)日:2022-05-03

    申请号:US16207721

    申请日:2018-12-03

    摘要: The present invention discloses a method for producing a long chain dicarboxylic acid by fermentation as well as a fermentation broth, a treated fermentation broth and a wastewater. The salt content in the fermentation broth is controlled to be below 20% by the fermentation method of the present invention. The method for producing a long chain dicarboxylic acid by fermentation provided by the present invention can effectively reduce the amount of alkali used in the fermentation process and the amount of acid used in the subsequent extraction of the long chain dicarboxylic acid, thereby reducing the amount of salt in the whole production process of the long chain dicarboxylic acid. At the same time, the method of the present invention also has many advantages such as shortening the fermentation time, increasing the acid production, reducing the amount of medium, and suitable for the production of various types of long-chain dicarboxylic acids, etc. Compared with the existing production process, the method of the present invention not only has significant cost advantages, but also can effectively reduce the pressure on resource and environment, thus it has a very obvious value advantage in industrialization.

    METHOD AND SYSTEM FOR EXTRACTING LONG CHAIN DICARBOXYLIC ACID

    公开(公告)号:US20220106249A1

    公开(公告)日:2022-04-07

    申请号:US17551333

    申请日:2021-12-15

    IPC分类号: C07C51/43 C07C55/02

    摘要: The present disclosure provides a method for extracting long chain dicarboxylic acid, the method comprising: (1) subjecting a long chain dicarboxylic acid fermentation broth to a primary membrane filtration treatment to give a first filtrate; subjecting the first filtrate to decolorization, crystallization, and solid-liquid separation to give a first solid; (2) redissolving the first solid in water to form a solution; subjecting the solution to decolorization, crystallization by acidification, and solid-liquid separation to give a second solid. By the method, the resulted long chain dicarboxylic acid product has a high purity and no residual organic solvent.

    Method and system for extracting long chain dicarboxylic acid

    公开(公告)号:US11229881B2

    公开(公告)日:2022-01-25

    申请号:US16887153

    申请日:2020-05-29

    摘要: The present disclosure provides a method and a system for extracting long chain dicarboxylic acid, the method comprising: (1) subjecting a long chain dicarboxylic acid fermentation broth to a primary membrane filtration treatment to give a first filtrate; subjecting the first filtrate to decolorization, acidification/crystallization, and solid-liquid separation treatments to give a first solid; (2) mixing the first solid, a base and water to form a solution; subjecting the solution to a secondary membrane filtration treatment to give a second filtrate; subjecting the second filtrate to decolorization, acidification/crystallization, and solid-liquid separation treatments to give a second solid; and (3) mixing the second solid and water to form a mixture; subjecting the mixture to a thermostatic treatment at 105-150° C., followed by cooling for crystallization and solid-liquid separation treatment. By the method, the resulted long chain dicarboxylic acid product has a high purity and no residual organic solvent.

    Long-chain dibasic acid with low content of monobasic acid impurity and the production method thereof

    公开(公告)号:US11225677B2

    公开(公告)日:2022-01-18

    申请号:US16503301

    申请日:2019-07-03

    IPC分类号: C12P7/64 C12N9/02

    摘要: The invention relates to a long-chain dibasic acid with low content of monobasic acid impurity and a production method thereof, in particular to the preparation of a long-chain dibasic acid producing strain by means of directed evolution and homologous recombination, and to the production of a long-chain dibasic acid with low content of monobasic acid impurity by fermentation of said strain. The invention relates to a mutated CYP52A12 gene, homologous gene or variant thereof, which, relative to GenBank Accession Number AY230498 and taking the first base upstream of the start codon ATG as −1, comprises a mutation. The invention relates to a strain comprising said mutated CYP52A12 gene, homologous gene or variant thereof wherein when the strain is fermented to produce a long-chain dibasic acid, the content of monobasic acid impurity in the fermentation product is significantly reduced.