-
公开(公告)号：US08329429B2
公开(公告)日：2012-12-11
申请号：US12626441
申请日：2009-11-25
CPC分类号： C12N9/6408 , C07K14/51
摘要： The present invention provides a method of enhanced protein production that comprises the step of expressing a recombinant gene encoding the protein in eukaryotic cells under conditions in which cleavage of the pro-domain of the protein is inhibited or eliminated. Generally the method of the present invention includes the step of inhibiting or altering the cleavage of a pro-domain of a recombinant protein of interest in order to increase the amount of recombinant protein secreted from a eukaryotic cell. Recombinant proteins that can be prepared using the method of this invention include members of the transforming growth factor-β (TGF-β) superfamily, such as bone morphogenetic proteins. Also provided are genetically engineered cells and polynucleotides for performing the method of the invention.
摘要翻译： 本发明提供增强蛋白质生产的方法，其包括在抑制或消除蛋白质前结构域的切割的条件下，在真核细胞中表达编码该蛋白质的重组基因的步骤。 通常，本发明的方法包括抑制或改变目的重组蛋白质的前结构域的切割以增加从真核细胞分泌的重组蛋白的量的步骤。 可以使用本发明的方法制备的重组蛋白质包括转化生长因子-bgr的成员; （TGF-β）超家族，如骨形态发生蛋白。 还提供了用于实施本发明的方法的遗传工程改造的细胞和多核苷酸。
-
2.发明申请USE OF IMMOBILIZED ANTAGONISTS FOR ENHANCING GROWTH FACTOR CONTAINING BIOIMPLANT EFFECTIVENESS 审中-公开使用固定拮抗剂来增强含有生物有效性的生长因子公开(公告)号：US20110182911A1
公开(公告)日：2011-07-28
申请号：US13002444
申请日：2011-01-03
IPC分类号： A61K39/395 , A61K38/18 , A61K38/30 , A61P43/00
CPC分类号： A61K38/1841 , A61K38/1875 , A61K38/30 , A61L27/24 , A61L27/32 , A61L27/34 , A61L27/54 , A61L2300/252 , A61L2300/256 , A61L2300/414 , A61L2300/432 , A61L2300/602
摘要： A method of retaining growth factors on a substrate for release at a treatment region comprises immobilizing an antagonist of the growth factor on a substrate and binding of the growth factor to the antagonist. In one aspect, the substrate is provided on a bioimplant. The resulting bioimplant allows for activity of the growth factor to continue at the region of implantation. According to the method of the invention, exogenous growth factors can be used to stimulate the repair of various tissues and organs at the site requiring repair, and be protected from inactivation, sequestration or degradation. The invention also provides bioimplants and methods of delivering growth factors.
摘要翻译： 将生长因子保留在底物上用于在治疗区域释放的方法包括将生长因子的拮抗剂固定在底物上并将生长因子与拮抗剂结合。 在一个方面，衬底被提供在生物植入物上。 所得到的生物植入物允许生长因子的活性在植入区域继续进行。 根据本发明的方法，可以使用外源性生长因子来刺激需要修复的部位的各种组织和器官的修复，并且防止其失活，螯合或降解。 本发明还提供生物植入物和递送生长因子的方法。
-
公开(公告)号：US06623748B2
公开(公告)日：2003-09-23
申请号：US09939898
申请日：2001-08-27
申请人： Cameron M. L. Clokie
发明人： Cameron M. L. Clokie
IPC分类号： A61F1300
CPC分类号： A61L27/3687 , A61L27/227 , A61L27/3608 , A61L27/3645 , A61L27/365 , A61L27/3683 , A61L2430/02
摘要： A biocompatible connective tissue repair composition which comprises a therapeutic material and a carrier comprising a means for achieving reverse phase characteristics, and methods for using said composition. The therapeutic material can be demineralized bone powder, and the carrier can be a poloxamer such as poloxamer 407.
-
4.发明授权Osseointegration promoting implant composition, implant assembly and method therefor 失效促进植入物组合物的植骨整合，植入物组装及其方法
公开(公告)号：US5503558A
公开(公告)日：1996-04-02
申请号：US151537
申请日：1993-11-12
申请人： Cameron M. L. Clokie
发明人： Cameron M. L. Clokie
CPC分类号： A61K38/1841 , A61C8/0018 , A61C8/005 , A61C8/0054 , A61C8/0069 , A61L27/227 , A61C8/006
摘要： A liquid composition comprising a transforming growth factor .beta. (TGF-.beta.) in a liquid carrier which gels at about 37.degree. C., promotes osseointegration of an implant in bone, when introduced into a bore in the bone, prior to insertion of the implant in the bone; a method of placing an implant in bone comprises injecting said liquid composition into a bore in the bone prior to insertion of the implant in the bore, the TGF-.beta. being present in the liquid composition in a concentration effective in an interspace between an inner surface of the bore and an outer surface of the implant, to promote osseointegration.
摘要翻译： 包含在约37℃凝胶的液体载体中的转化生长因子β（TGF-β）的液体组合物在插入植入物之前促进植入物在骨中的引入到骨中的孔中的骨整合 在骨头里 将植入物放置在骨中的方法包括在将植入物插入孔内之前将所述液体组合物注入骨中的孔中，所述TGF-β以浓度有效地存在于内表面 的孔和植入物的外表面，以促进骨整合。
-
公开(公告)号：US06287312B1
公开(公告)日：2001-09-11
申请号：US09521849
申请日：2000-03-09
IPC分类号： A61B1700
CPC分类号： A61F2/4644 , A61B2050/0064 , A61F2002/2835 , A61F2002/30616 , A61F2002/30716 , A61F2002/4645 , A61F2250/0064 , A61F2250/009 , B02C19/0056 , B02C19/20
摘要： A bone mill comprising a vessel and a rotatable grinding tool to produce ground autologous bone particles for surgical bone reconstruction use, wherein the vessel includes a wall defining a circular cylindrical chamber having a concentric axis. The vessel further includes a bottom wall at right angle to the axis and a lid adapted to be removably connected to the vessel. The lid includes an opening coincident with the axis. The grinding tool includes an elongated shaft extending through the opening in the lid along the axis with a cutting blade mounted to the shaft within the chamber whereby the cutting blade can be rotated by motive device, such as a dental drill, engaging the shaft exterior of the vessel to cut bone, placed in the vessel, into said bone particles.
摘要翻译： 一种骨磨机，其包括容器和可旋转研磨工具，以产生用于外科手术骨重建用途的磨碎的自体骨颗粒，其中所述容器包括限定具有同心轴线的圆柱形腔室的壁。 容器还包括与轴线成直角的底壁和适于可移除地连接到容器的盖子。 盖子包括与轴线重合的开口。 研磨工具包括细长轴，其沿着轴线延伸穿过盖中的开口，并且具有安装到腔室内的轴的切割刀片，由此切割刀片可以通过诸如牙科钻头的动力装置旋转，所述动力装置接合轴的外部 将骨头放入容器中的容器进入所述骨颗粒。
-
公开(公告)号：US09675670B2
公开(公告)日：2017-06-13
申请号：US14111518
申请日：2012-04-11
IPC分类号： A61K9/52 , A61K9/54 , A61K38/18 , A61K38/19 , A61K38/22 , A61K9/50 , A61K9/00 , A61K47/34 , A61K9/16 , A61K47/02 , A61L27/46 , A61L27/54 , A61K47/10 , A61K47/48 , A61K9/19
CPC分类号： A61K38/1875 , A61K9/0024 , A61K9/1611 , A61K9/1641 , A61K9/1682 , A61K9/19 , A61K9/5084 , A61K38/18 , A61K38/1825 , A61K38/1841 , A61K38/1858 , A61K38/1866 , A61K47/02 , A61K47/10 , A61K47/34 , A61K47/48861 , A61K47/6923 , A61L27/46 , A61L27/54 , C08L67/04 , C08L71/02
摘要： A system for multiphasic delivery of at least one growth factor at a treatment site comprises a delivery vehicle for releasing at least one growth factor in an initial release profile and a carrier for releasing at least one growth factor in a sustained release profile. The initial release profile releases at least one growth factor over a period of hours to days, wherein the growth factor is released in a large amount initially, with the remainder being released in progressively lower amounts. The sustained release profile releases at least one growth factor over a period of days to weeks, wherein the growth factor is released at a generally constant amount over such period. The system of the invention is particularly suited for applications on bioimplants. The invention also comprises methods and kits for multiphasic delivery of at least one growth factor.
-
公开(公告)号：US06309659B1
公开(公告)日：2001-10-30
申请号：US08922068
申请日：1997-09-02
申请人： Cameron M. L. Clokie
发明人： Cameron M. L. Clokie
IPC分类号： A61F1300
CPC分类号： A61L27/3687 , A61L27/227 , A61L27/3608 , A61L27/3645 , A61L27/365 , A61L27/3683 , A61L2430/02
摘要： A biocompatible connective tissue repair composition which comprises a therapeutic material and a carrier comprising a means for achieving reverse phase characteristics, and methods for using said composition. The therapeutic material can be demineralized bone powder, and the carrier can be a poloxamer such as poloxamer 407.
摘要翻译： 一种生物相容性结缔组织修复组合物，其包含治疗材料和载体，其包含用于实现反相特征的装置，以及使用所述组合物的方法。 治疗材料可以是软化骨粉末，载体可以是泊洛沙姆如泊洛沙姆407。
-
-
-
-
-
-
搜索结果
7 条记录 (耗时 0.011 秒)