公开(公告)号：US20050100969A1

公开(公告)日：2005-05-12

申请号：US10959296

申请日：2004-10-07

申请人： Li-Te Yin ,  Chao-Yun Tsao ,  Chung-We Pan ,  Su-Fung Chiou ,  Zheng-Cheng Chen

发明人： Li-Te Yin ,  Chao-Yun Tsao ,  Chung-We Pan ,  Su-Fung Chiou ,  Zheng-Cheng Chen

IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/54393

摘要： The present invention provides a substrate for protein microarrays, whereby compound A and GPTS are mixed for coating onto a solid support to form a layer, wherein said compound A is selected from a group consisting of nitrocellulose, poly(styrene-co-maleic anhydride) and polyvinylidene fluoride. Moreover, the present invention also provides a protein microarray by depositing proteins on said layer of said substrate.

摘要翻译： 本发明提供了用于蛋白质微阵列的底物，其中化合物A和GPTS被混合以涂覆在固体支持物上以形成层，其中所述化合物A选自硝酸纤维素，聚（苯乙烯 - 共 - 马来酸酐） 和聚偏二氟乙烯。 此外，本发明还通过在所述基底的所述层上沉积蛋白来提供蛋白质微阵列。

公开(公告)号：US07713749B2

公开(公告)日：2010-05-11

申请号：US10959296

申请日：2004-10-07

申请人： Li Te Yin ,  Chao Yun Tsao ,  Chung We Pan ,  Su Fung Chiou ,  Zheng Cheng Chen

发明人： Li Te Yin ,  Chao Yun Tsao ,  Chung We Pan ,  Su Fung Chiou ,  Zheng Cheng Chen

IPC分类号： G01N33/543

CPC分类号： G01N33/54393

摘要： The present invention provides a substrate for protein microarrays, whereby compound A and GPTS are mixed for coating onto a solid support to form a layer, wherein said compound A is selected from a group consisting of nitrocellulose, poly(styrene-co-maleic anhydride) and polyvinylidene fluoride. Moreover, the present invention also provides a protein microarray by depositing proteins on said layer of said substrate.

摘要翻译： 本发明提供了用于蛋白质微阵列的底物，其中化合物A和GPTS被混合以涂覆在固体支持物上以形成层，其中所述化合物A选自硝酸纤维素，聚（苯乙烯 - 共 - 马来酸酐） 和聚偏二氟乙烯。 此外，本发明还通过在所述基底的所述层上沉积蛋白来提供蛋白质微阵列。

公开(公告)号：US20050106649A1

公开(公告)日：2005-05-19

申请号：US10964713

申请日：2004-10-15

申请人： Chao-Yun Tsao ,  Li-Te Yin ,  Su-Fung Chiou ,  Chung-We Pan ,  Jia-Huey Tsao

发明人： Chao-Yun Tsao ,  Li-Te Yin ,  Su-Fung Chiou ,  Chung-We Pan ,  Jia-Huey Tsao

IPC分类号： C12N9/00 ,  C12Q1/37 ,  C12Q1/66 ,  G01N33/50 ,  G01N33/53

CPC分类号： G01N33/5306

摘要： Polyallylamine conjugates and applications thereof for biological signal amplification are provided by utilizing the essential amino group of polyallylamine to covalently bind with capture agents and signal molecules having the functional groups selected from a group consisting of —NHS, —CO, —S═O2 and —C═O—C═O. The resulting conjugates having more than one signaling entities can be further implemented for biological expression with enhancing effect on biological signal intensity, such that the sensitivity of detection for the variation between biological interactions is largely increased.

摘要翻译： 通过利用聚烯丙胺的必需氨基与捕获剂和具有选自-NHS，-CO，-SO 2的官能团的信号分子共价结合的聚烯丙基胺共轭物及其应用来提供生物信号扩增 和-COCO。 具有多于一个信号实体的所得缀合物可以进一步实现用于生物表达，对生物信号强度具有增强的作用，使得检测对生物相互作用之间变化的敏感性大大增加。

公开(公告)号：US20050106607A1

公开(公告)日：2005-05-19

申请号：US10965792

申请日：2004-10-18

申请人： Li-Te Yin ,  Chih-Yu Hu ,  Chao-Yun Tsao ,  Su-Fung Chiou ,  Jia-Huey Tsao

发明人： Li-Te Yin ,  Chih-Yu Hu ,  Chao-Yun Tsao ,  Su-Fung Chiou ,  Jia-Huey Tsao

IPC分类号： B01L3/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/53 ,  G01N33/543

CPC分类号： B01L3/5085 ,  B01J2219/00317 ,  B01J2219/00533 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0887 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/54386

摘要： This invention is provided a biochip containing splitable reaction wells and method for producing same and application thereof. The invention avoids cross contamination or interference between different samples on the same biochip. In addition, the soft polymer film removed from reaction wells may be reused on different substrates, thereby saving the cost of experiment or clinical testing.

摘要翻译： 本发明提供了一种含有可分裂反应孔的生物芯片及其生产方法及其应用。 本发明避免了相同生物芯片上不同样品之间的交叉污染或干扰。 此外，从反应孔中除去的软聚合物膜可以重复使用在不同的基底上，从而节省了实验或临床测试的成本。

公开(公告)号：US07811571B2

公开(公告)日：2010-10-12

申请号：US10964713

申请日：2004-10-15

申请人： Chao Yun Tsao ,  Li Te Yin ,  Su Fung Chiou ,  Chung We Pan ,  Jia Huey Tsao

发明人： Chao Yun Tsao ,  Li Te Yin ,  Su Fung Chiou ,  Chung We Pan ,  Jia Huey Tsao

IPC分类号： A61K39/00

CPC分类号： G01N33/5306

摘要： Polyallylamine conjugates and applications thereof for biological signal amplification are provided by utilizing the essential amino group of polyallylamine to covalently bind with capture agents and signal molecules having the functional groups selected from a group consisting of —NHS, —CO, —S═O2 and —C═O—C═O. The resulting conjugates having more than one signaling entities can be further implemented for biological expression with enhancing effect on biological signal intensity, such that the sensitivity of detection for the variation between biological interactions is largely increased.

摘要翻译： 聚烯丙胺共轭物及其在生物信号扩增中的应用通过利用聚烯丙胺的必需氨基与捕获剂共价结合并与具有选自-NHS，-CO，-S = O 2和 - C = O-C = O。 具有多于一个信号实体的所得缀合物可以进一步实现用于生物表达，对生物信号强度具有增强的作用，使得检测对生物相互作用之间变化的敏感性大大增加。