公开(公告)号：US20090022629A1

公开(公告)日：2009-01-22

申请号：US11778674

申请日：2007-07-17

申请人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Nien-Hsuan Chou ,  Chung-We Pan

发明人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Nien-Hsuan Chou ,  Chung-We Pan

IPC分类号： G01N27/333 ,  B01J19/00

CPC分类号： G01N33/493 ,  G01N33/54373 ,  G01N33/84

摘要： A solid-state urea biosensor may comprise a substrate, an electrically conductive layer, a PH sensing film, an ammonium ion selecting film and a ferment film. The electrically conductive layer covers the substrate. The PH sensing film has a PH value measuring zone, partially covering the electrically conductive layer, for measuring the PH value of a solution to be tested. The ammonium ion selecting film has an ammonium ion measuring zone for measuring the ammonium ion concentration. The ammonium ion selecting film partially covers the PH sensing film and exposes the PH value measuring zone. The ferment film is used for measuring the urea concentration in the solution, wherein the ferment film partially covers the ammonium ion selecting film and exposes the ammonium ion measuring zone.

摘要翻译： 固态尿素生物传感器可以包括基底，导电层，PH感测膜，铵离子选择膜和发酵膜。 导电层覆盖基板。 PH感测膜具有部分覆盖导电层的PH值测量区，用于测量待测溶液的PH值。 铵离子选择膜具有用于测量铵离子浓度的铵离子测量区。 铵离子选择膜部分地覆盖PH感测膜并暴露PH值测量区。 发酵膜用于测量溶液中的尿素浓度，其中发酵膜部分地覆盖铵离子选择膜并暴露铵离子测量区。

公开(公告)号：US20080099331A1

公开(公告)日：2008-05-01

申请号：US11306809

申请日：2006-01-12

申请人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan

发明人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan

IPC分类号： G01N33/487 ,  G01N27/26

CPC分类号： C12Q1/58

摘要： The invention provides a method of fabrication of a solid-state urea biosensor and its data acquisition system. The biosensor includes a substrate, and three individual sensing areas separated by an insulating layer on the substrate. Each individual sensing area contains a conductive layer on the substrate, and a pH sensitive membrane is deposited thereon. An enzyme layer is deposited on one of the pH sensitive membranes to form a working electrode. The other two sensing areas are a quasi-reference electrode and a contrast electrode, respectively. The sensing signals are transferred to an instrumentation amplifier by conductive wires. The sensing signals are then transferred to a low-pass filter to reduce the high frequency noise. A data acquisition card is employed to transfer the sensing signals to a computer. The sensing signals are analyzed by the program of the data acquisition system. The data are then displayed on the display panel.

摘要翻译： 本发明提供一种固态尿素生物传感器及其数据采集系统的制造方法。 生物传感器包括基板和由基板上的绝缘层分隔开的三个单独的感测区域。 每个单独的感测区域在衬底上包含导电层，并且在其上沉积pH敏感膜。 酶层沉积在一个pH敏感膜上以形成工作电极。 其他两个感测区域分别是准参考电极和对比电极。 传感信号通过导线传输到仪表放大器。 然后将感测信号传送到低通滤波器以降低高频噪声。 使用数据采集卡将感测信号传送到计算机。 感测信号由数据采集系统的程序进行分析。 然后将数据显示在显示面板上。

公开(公告)号：US20060011951A1

公开(公告)日：2006-01-19

申请号：US10891569

申请日：2004-07-15

申请人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan ,  I-Kone Kao

发明人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan ,  I-Kone Kao

IPC分类号： H01L29/80 ,  H01L21/8238

CPC分类号： G01N27/414

摘要： A potassium/sodium ion sensing device applying an extended-gate field effect transistor, which using an extended-gate ion sensitive field effect transistor (EGFET) as base to fabricate a potassium/sodium ion sensing device, using the extended gate of the extended-gate ion sensitive field effect transistor as a signal intercept electrode, and immobilizing the hydro-aliphatic urethane diacrylate (EB2001) intermixed with electronegative additive, potassium ionophore, sodium ionophore, and the like, to fabricate a potassium/sodium ion sensing electrode. The present invention utilizes the photocurability and good hydrophilicity of the hydro-aliphatic urethane diacrylate (EB2001), and fixes potassium/sodium ionophore, can obtain a non-wave filter, single-layer, stable signal potassium and sodium ion sensor. Thus, when the present invention is applied to measure the concentration of potassium/sodium ions in a sample, the mutual interference between potassium/sodium ion electrodes can be reduced, so the measured value can be more close to the actual value.

摘要翻译： 一种使用扩展栅极离子敏感场效应晶体管（EGFET）作为基极的扩展栅极场效应晶体管的钾/钠离子感测装置，使用扩展栅极场效应晶体管的扩展栅极， 栅极离子敏感场效应晶体管作为信号截距电极，并将与负电性添加剂，钾离子载体，钠离子载体等混合的氢 - 脂族氨基甲酸酯二丙烯酸酯（EB2001）固定，制造钾/钠离子感测电极。 本发明利用了水解脂肪族聚氨酯二丙烯酸酯（EB2001）的光固化性和亲水性，并且固定了钾/钠离子载体，可以得到非波长的过滤器，单层，稳定的信号钾离子和钠离子传感器。 因此，当本发明用于测量样品中钾/钠离子的浓度时，可以减少钾离子/钠离子电极之间的相互干扰，因此测量值可以更接近实际值。

公开(公告)号：US20050147741A1

公开(公告)日：2005-07-07

申请号：US10750073

申请日：2003-12-31

申请人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan ,  Jing-Sheng Chiang

发明人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan ,  Jing-Sheng Chiang

IPC分类号： H01L21/00

CPC分类号： G01N27/4148

摘要： A method for fabricating an array pH sensor and a readout circuit device of such array pH sensor are implemented by utilizing an extended ion sensitive field effect transistor to construct the array pH sensor and related readout circuit. The structure of the array sensor having this extended ion sensitive field effect transistor comprises a tin dioxide/metal/silicon dioxide multi-layer structure sensor and a tin dioxide/indium tin oxide/glass multi-layer structure sensor and has excellent properties. Furthermore, the readout circuit and the sensor utilize two signal generators for controlling and reading signals. In particular, the sensor can be effective for increasing the accuracy of measurement and reducing the interference of noise.

摘要翻译： 通过利用扩展的离子敏感场效应晶体管构成阵列pH传感器和相关读出电路来实现阵列pH传感器的制造方法和这种阵列pH传感器的读出电路装置。 具有该扩展离子敏感场效应晶体管的阵列传感器的结构包括二氧化锡/金属/二氧化硅多层结构传感器和二氧化锡/铟锡氧化物/玻璃多层结构传感器，并且具有优异的性能。 此外，读出电路和传感器利用两个信号发生器来控制和读取信号。 特别地，传感器可以有效地提高测量的精度并降低噪声的干扰。

公开(公告)号：US20050147736A1

公开(公告)日：2005-07-07

申请号：US10750072

申请日：2003-12-31

申请人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan

发明人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan

IPC分类号： A61L2/00 ,  B05D5/12 ,  G01N27/333 ,  G01N27/403

CPC分类号： G01N27/4035 ,  G01N27/3335

摘要： A process for fabricating a whole solid-state pH sensing device by using the polypyrrole as the contrast pH detector and a whole solid-state pH sensing device fabricated by the process are disclosed, wherein said device is a differential pair framework potential electrochemical sensing device fabricated by using a non-insulating solid-state inorganic ion-sensing membrane and a polypyrrole sensing membrane. The largest difference between the device of the present invention and the conventional potentiometric type pH sensor is that the sensor of the invention is a solid-state planar sensor. The differential pair framework uses tin dioxide as the ion-sensing membrane and the reference electrode, and uses a polypyrrole sensor as the differential sensor, wherein the sensitivity of tin dioxide is good and has a value up to 57 mV/pH, and the sensitivity of polypyrrole is about 27 mV/pH. These lead the sensitivity of the whole solid-state pH Sensing device to a value of 30 mV/pH and exhibits good linearity, so that the sensing device framework has practicability. Since the sensitivity of the polypyrrole can be controlled by means of its polymerization, a sensing device with controllable sensitivity can be fabricated for applying to the fabrication of a pH sensor or a biosensor.

摘要翻译： 公开了一种通过使用聚吡咯作为对比pH检测器和通过该方法制造的整个固态pH感测装置来制造整个固态pH感测装置的方法，其中所述装置是制造的差分对框架电位电化学感测装置 通过使用非绝缘固态无机离子感测膜和聚吡咯感测膜。 本发明装置与常规电位式pH传感器之间的最大差异在于本发明的传感器是固态平面传感器。 差分对框架使用二氧化锡作为离子感测膜和参比电极，并使用聚吡咯传感器作为差示传感器，其中二氧化锡的灵敏度良好，并且具有高达57mV / pH的值，灵敏度 的聚吡咯约为27mV / pH。 这些将整个固态pH传感装置的灵敏度提高到30 mV / pH值，呈现出良好的线性度，使感应装置框架具有实用性。 由于可以通过其聚合来控制聚吡咯的灵敏度，因此可以制造具有可控感光度的感测装置，用于制造pH传感器或生物传感器。

公开(公告)号：US07736478B2

公开(公告)日：2010-06-15

申请号：US11515727

申请日：2006-09-06

申请人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan ,  Chin-Shuen Hung

发明人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan ,  Chin-Shuen Hung

IPC分类号： G01N27/333

CPC分类号： G01N27/4035

摘要： An ion solution concentration-detecting device includes a working electrode, a reference electrode, and a link element. The working electrode and the reference electrode are inserted into the link element. The working electrode, inserted into the link element, is replaceable, for the better sensitivity to the different ion solution. The working electrode being a contact-detecting electrode can be replaced by another contact-detecting electrode with different sensing membrane. The reference electrode includes a polymeric membrane with ion material covering silver/silver chloride (Ag/AgCl) electrode to provide a fixed and stable electrical potential. The working electrode may be a contact-detecting electrode having a sensing membrane. The sensing membrane may be a metal oxide or a polymer with ion material for measuring different ion solution effectively.

摘要翻译： 离子溶液浓度检测装置包括工作电极，参考电极和连接元件。 工作电极和参考电极插入到连接元件中。 插入连接元件的工作电极是可更换的，以便更好地对不同离子溶液的敏感性。 作为接触检测电极的工作电极可以由具有不同传感膜的另一接触检测电极代替。 参考电极包括具有覆盖银/氯化银（Ag / AgCl）电极的离子材料的聚合物膜，以提供固定和稳定的电势。 工作电极可以是具有感测膜的接触检测电极。 感测膜可以是金属氧化物或具有用于有效测量不同离子溶液的离子材料的聚合物。

公开(公告)号：US07638157B2

公开(公告)日：2009-12-29

申请号：US11533358

申请日：2006-09-19

申请人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Wen-Yaw Chung ,  Li-Te Yin ,  Chung-We Pan

发明人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Wen-Yaw Chung ,  Li-Te Yin ,  Chung-We Pan

IPC分类号： G01N1/28

CPC分类号： C12Q1/006 ,  C12N9/0006 ,  C12N11/08

摘要： A method of fabricating an electrode assembly of a sensor is described. The sensor has a field effect transistor. The electrode assembly is separated from the field effect transistor by only a conductive line. The sensor is functioned to detect different glucose concentrations. A solid layer of tin oxide is deposited on a substrate board. A β-D-glucose oxidase and polyvinyl alcohol bearing styrylpyridinium groups are placed in 100 μl of sulfuric acid, to form an enzyme mixture. The enzyme mixture is dropped on the solid layer of tin oxide. The enzyme mixture is dried. The enzyme mixture is exposed to a UV ray. The enzyme mixture is dried and stabilized. The enzyme mixture is immersed in a sulfuric buffer.

摘要翻译： 描述了制造传感器的电极组件的方法。 该传感器具有场效应晶体管。 电极组件仅通过导线与场效应晶体管分离。 该传感器用于检测不同的葡萄糖浓度。 在基板上沉积固体氧化锡层。 将β-D-葡萄糖氧化酶和含有苯乙烯基吡啶鎓基团的聚乙烯醇基团置于100毫升硫酸中以形成酶混合物。 将酶混合物滴在氧化锡的固体层上。 将酶混合物干燥。 将酶混合物暴露于紫外线。 将酶混合物干燥并稳定。 将酶混合物浸入硫酸缓冲液中。

公开(公告)号：US20060021874A1

公开(公告)日：2006-02-02

申请号：US11137551

申请日：2005-05-26

申请人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan ,  Zheng-Cheng Chen

发明人： Shen-Kan Hsiung ,  Jung-Chuan Chou ,  Tai-Ping Sun ,  Chung-We Pan ,  Zheng-Cheng Chen

IPC分类号： G01N27/327 ,  H01R43/16

CPC分类号： G01N27/401 ,  Y10T29/49206

摘要： In this present invention it was fabricated to be relates to manufacturing a ceramic interface electrochemical reference electrode for use together with biomedical sensors. Most potentiometric biomedical sensors must have the need to be connected to a reference electrode to offer the readout circuit a stable voltage in the different solutions when measuring for providing that can provide a standard comparing voltage to avoid measuring errors caused by an unstable environment. Usually, However, the presently available commercial reference electrode we used is too big in size and inconvenient to store. For this reason we develop the ceramic interface electrochemical reference electrode which can minimize volume and need not to be preserved in the saturated solution for biosensor. Therefore, the ceramic interface electrochemical reference electrode of the present invention does not need to be stored in solution and can be minimized for use in future sensors. In addition, the present invention also relates to a ceramic interface electrochemical reference electrode.

摘要翻译： 在本发明中，其制造涉及制造与生物医学传感器一起使用的陶瓷界面电化学参考电极。 大多数电位生物医学传感器必须需要连接到参考电极，以便在测量时提供不同解决方案中的稳定电压，以提供可提供标准比较电压以避免由不稳定环境引起的测量误差。 通常，然而，我们使用的目前可用的商业参考电极的尺寸太大并且不方便存储。 为此，我们开发了陶瓷界面电化学参比电极，可以使体积最小化，不需要保留在生物传感器的饱和溶液中。 因此，本发明的陶瓷界面电化学参考电极不需要存储在溶液中，并且可以最小化以用于将来的传感器。 此外，本发明还涉及陶瓷界面电化学参考电极。

公开(公告)号：US20050100969A1

公开(公告)日：2005-05-12

申请号：US10959296

申请日：2004-10-07

申请人： Li-Te Yin ,  Chao-Yun Tsao ,  Chung-We Pan ,  Su-Fung Chiou ,  Zheng-Cheng Chen

发明人： Li-Te Yin ,  Chao-Yun Tsao ,  Chung-We Pan ,  Su-Fung Chiou ,  Zheng-Cheng Chen

IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/54393

摘要： The present invention provides a substrate for protein microarrays, whereby compound A and GPTS are mixed for coating onto a solid support to form a layer, wherein said compound A is selected from a group consisting of nitrocellulose, poly(styrene-co-maleic anhydride) and polyvinylidene fluoride. Moreover, the present invention also provides a protein microarray by depositing proteins on said layer of said substrate.

摘要翻译： 本发明提供了用于蛋白质微阵列的底物，其中化合物A和GPTS被混合以涂覆在固体支持物上以形成层，其中所述化合物A选自硝酸纤维素，聚（苯乙烯 - 共 - 马来酸酐） 和聚偏二氟乙烯。 此外，本发明还通过在所述基底的所述层上沉积蛋白来提供蛋白质微阵列。

公开(公告)号：US07713749B2

公开(公告)日：2010-05-11

申请号：US10959296

申请日：2004-10-07

申请人： Li Te Yin ,  Chao Yun Tsao ,  Chung We Pan ,  Su Fung Chiou ,  Zheng Cheng Chen

发明人： Li Te Yin ,  Chao Yun Tsao ,  Chung We Pan ,  Su Fung Chiou ,  Zheng Cheng Chen

IPC分类号： G01N33/543

CPC分类号： G01N33/54393

摘要： The present invention provides a substrate for protein microarrays, whereby compound A and GPTS are mixed for coating onto a solid support to form a layer, wherein said compound A is selected from a group consisting of nitrocellulose, poly(styrene-co-maleic anhydride) and polyvinylidene fluoride. Moreover, the present invention also provides a protein microarray by depositing proteins on said layer of said substrate.

摘要翻译： 本发明提供了用于蛋白质微阵列的底物，其中化合物A和GPTS被混合以涂覆在固体支持物上以形成层，其中所述化合物A选自硝酸纤维素，聚（苯乙烯 - 共 - 马来酸酐） 和聚偏二氟乙烯。 此外，本发明还通过在所述基底的所述层上沉积蛋白来提供蛋白质微阵列。