Polyallylamine conjugates and applications thereof for biological signal amplification
    2.
    发明申请
    Polyallylamine conjugates and applications thereof for biological signal amplification 有权
    聚烯丙胺共轭物及其在生物信号扩增中的应用

    公开(公告)号:US20050106649A1

    公开(公告)日:2005-05-19

    申请号:US10964713

    申请日:2004-10-15

    CPC分类号: G01N33/5306

    摘要: Polyallylamine conjugates and applications thereof for biological signal amplification are provided by utilizing the essential amino group of polyallylamine to covalently bind with capture agents and signal molecules having the functional groups selected from a group consisting of —NHS, —CO, —S═O2 and —C═O—C═O. The resulting conjugates having more than one signaling entities can be further implemented for biological expression with enhancing effect on biological signal intensity, such that the sensitivity of detection for the variation between biological interactions is largely increased.

    摘要翻译: 通过利用聚烯丙胺的必需氨基与捕获剂和具有选自-NHS,-CO,-SO 2的官能团的信号分子共价结合的聚烯丙基胺共轭物及其应用来提供生物信号扩增 和-COCO。 具有多于一个信号实体的所得缀合物可以进一步实现用于生物表达,对生物信号强度具有增强的作用,使得检测对生物相互作用之间变化的敏感性大大增加。

    Substrate for fabricating protein microarrays
    3.
    发明申请
    Substrate for fabricating protein microarrays 有权
    用于制造蛋白质微阵列的底物

    公开(公告)号:US20050100969A1

    公开(公告)日:2005-05-12

    申请号:US10959296

    申请日:2004-10-07

    CPC分类号: G01N33/54393

    摘要: The present invention provides a substrate for protein microarrays, whereby compound A and GPTS are mixed for coating onto a solid support to form a layer, wherein said compound A is selected from a group consisting of nitrocellulose, poly(styrene-co-maleic anhydride) and polyvinylidene fluoride. Moreover, the present invention also provides a protein microarray by depositing proteins on said layer of said substrate.

    摘要翻译: 本发明提供了用于蛋白质微阵列的底物,其中化合物A和GPTS被混合以涂覆在固体支持物上以形成层,其中所述化合物A选自硝酸纤维素,聚(苯乙烯 - 共 - 马来酸酐) 和聚偏二氟乙烯。 此外,本发明还通过在所述基底的所述层上沉积蛋白来提供蛋白质微阵列。

    Method of fabricating electrode assembly of sensor
    4.
    发明授权
    Method of fabricating electrode assembly of sensor 失效
    制造传感器电极组件的方法

    公开(公告)号:US07638157B2

    公开(公告)日:2009-12-29

    申请号:US11533358

    申请日:2006-09-19

    IPC分类号: G01N1/28

    摘要: A method of fabricating an electrode assembly of a sensor is described. The sensor has a field effect transistor. The electrode assembly is separated from the field effect transistor by only a conductive line. The sensor is functioned to detect different glucose concentrations. A solid layer of tin oxide is deposited on a substrate board. A β-D-glucose oxidase and polyvinyl alcohol bearing styrylpyridinium groups are placed in 100 μl of sulfuric acid, to form an enzyme mixture. The enzyme mixture is dropped on the solid layer of tin oxide. The enzyme mixture is dried. The enzyme mixture is exposed to a UV ray. The enzyme mixture is dried and stabilized. The enzyme mixture is immersed in a sulfuric buffer.

    摘要翻译: 描述了制造传感器的电极组件的方法。 该传感器具有场效应晶体管。 电极组件仅通过导线与场效应晶体管分离。 该传感器用于检测不同的葡萄糖浓度。 在基板上沉积固体氧化锡层。 将β-D-葡萄糖氧化酶和含有苯乙烯基吡啶鎓基团的聚乙烯醇基团置于100毫升硫酸中以形成酶混合物。 将酶混合物滴在氧化锡的固体层上。 将酶混合物干燥。 将酶混合物暴露于紫外线。 将酶混合物干燥并稳定。 将酶混合物浸入硫酸缓冲液中。

    METHOD OF FABRICATING ELECTRODE ASSEMBLY OF SENSOR
    5.
    发明申请
    METHOD OF FABRICATING ELECTRODE ASSEMBLY OF SENSOR 失效
    制造传感器电极组件的方法

    公开(公告)号:US20070023286A1

    公开(公告)日:2007-02-01

    申请号:US11533358

    申请日:2006-09-19

    摘要: A method of fabricating an electrode assembly of a sensor is described. The sensor has a field effect transistor. The electrode assembly is separated from the field effect transistor by only a conductive line. The sensor is functioned to detect different glucose concentrations. A solid layer of tin oxide is deposited on a substrate board. A β-D-glucose oxidase and polyvinylalchol bearing styrylpyridinium groups are placed in 100 μl of sulfuric acid, to form an enzyme mixture. The enzyme mixture is dropped on the solid layer of tin oxide. The enzyme mixture is dried. The enzyme mixture is exposed to a UV ray. The enzyme mixture is dried and stabilized. The enzyme mixture is immersed in a sulfuric buffer.

    摘要翻译: 描述了制造传感器的电极组件的方法。 传感器具有场效应晶体管。 电极组件仅通过导线与场效应晶体管分离。 该传感器用于检测不同的葡萄糖浓度。 在基板上沉积固体氧化锡层。 将含有苯乙烯基吡啶鎓基团的β-D-葡萄糖氧化酶和聚乙烯基基团置于100毫升硫酸中以形成酶混合物。 将酶混合物滴在氧化锡的固体层上。 将酶混合物干燥。 将酶混合物暴露于紫外线。 将酶混合物干燥并稳定。 将酶混合物浸入硫酸缓冲液中。