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1.发明授权Extract of E. coli cells having mutation in ribosomal protein S12, and method for producing protein in cell-free system using the extract 失效在核糖体蛋白S12中具有突变的大肠杆菌细胞的提取物，以及使用提取物在无细胞系统中产生蛋白质的方法公开(公告)号：US08603774B2
公开(公告)日：2013-12-10
申请号：US11137395
申请日：2005-05-26
申请人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
发明人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
IPC分类号： C12P21/02
CPC分类号： C12P21/02
摘要： It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
摘要翻译： 旨在提供在无细胞系统中合成蛋白质的提取物，试剂盒和方法。 提取物的特征在于由在核糖体蛋白S12基因中具有突变的突变型大肠杆菌细胞制备。 在一个优选的实施方案中，突变是这样的突变，其赋予对大肠杆菌对链霉素的抗性或依赖性，从而可以提高核糖体上的mRNA密码子的阅读效率，从而提高蛋白质生产力。
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2.发明授权Extract of E. coli cells having mutation in ribosomal protein S12, and method for producing protein in cell-free system using the extract 失效在核糖体蛋白S12中具有突变的大肠杆菌细胞的提取物，以及使用提取物在无细胞系统中产生蛋白质的方法公开(公告)号：US08623626B2
公开(公告)日：2014-01-07
申请号：US12912724
申请日：2010-10-26
申请人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
发明人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
CPC分类号： C12P21/02
摘要： It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
摘要翻译： 旨在提供在无细胞系统中合成蛋白质的提取物，试剂盒和方法。 提取物的特征在于由在核糖体蛋白S12基因中具有突变的突变型大肠杆菌细胞制备。 在一个优选的实施方案中，突变是这样的突变，其赋予对大肠杆菌对链霉素的抗性或依赖性，从而可以提高核糖体上的mRNA密码子的阅读效率，从而提高蛋白质生产力。
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3.发明申请EXTRACT OF E. COLI CELLS HAVING MUTATION IN RIBOSOMAL PROTEIN S12, AND METHOD FOR PRODUCING PROTEIN IN CELL-FREE SYSTEM USING THE EXTRACT 失效提取RIBOSOMAL蛋白S12中的突变的大肠杆菌细胞的提取，以及使用提取物在无细胞系统中生产蛋白质的方法公开(公告)号：US20110136204A1
公开(公告)日：2011-06-09
申请号：US12912724
申请日：2010-10-26
申请人： Namtip CHUMPOLKULWONG , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
发明人： Namtip CHUMPOLKULWONG , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
CPC分类号： C12P21/02
摘要： It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
摘要翻译： 旨在提供在无细胞系统中合成蛋白质的提取物，试剂盒和方法。 提取物的特征在于由在核糖体蛋白S12基因中具有突变的突变型大肠杆菌细胞制备。 在一个优选的实施方案中，突变是这样的突变，其赋予对大肠杆菌对链霉素的抗性或依赖性，从而可以提高核糖体上的mRNA密码子的阅读效率，从而提高蛋白质生产力。
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公开(公告)号：US20060008871A1
公开(公告)日：2006-01-12
申请号：US11137395
申请日：2005-05-26
申请人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
发明人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
CPC分类号： C12P21/02
摘要： It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
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