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1.发明授权Process for producing extract for cell-free protein synthesis and cell extract produced thereby 有权用于生产无细胞蛋白质合成提取物的方法和由此产生的细胞提取物公开(公告)号：US07615344B2
公开(公告)日：2009-11-10
申请号：US11282613
申请日：2005-11-21
申请人： Natsuko Matsuda , Takanori Kigawa , Namtip Chumpolkulwong , Chie Takemoto , Mikako Shirouzu , Akiko Tanaka , Shigeyuki Yokoyama
发明人： Natsuko Matsuda , Takanori Kigawa , Namtip Chumpolkulwong , Chie Takemoto , Mikako Shirouzu , Akiko Tanaka , Shigeyuki Yokoyama
IPC分类号： C12Q1/68
摘要： It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The above-described cells are preferably bacterial cells, in particular, Escherichia coli cells. In the suppressed growth conditions as described above, the culture temperature is preferably from 20 to 32° C., more preferably 26° C. or higher and lower than 30° C.
摘要翻译： 旨在提供一种用于生产无细胞蛋白质合成提取物的方法，由此可以提高蛋白质的生产率和生产效率。 细胞在抑制生长条件下培养。 在培养物的固定相中，收集细胞，然后破碎。 上述细胞优选为细菌细胞，特别是大肠杆菌细胞。 在上述抑制生长条件下，培养温度优选为20〜32℃，更优选为26℃以上且低于30℃。
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2.发明授权Extract of E. coli cells having mutation in ribosomal protein S12, and method for producing protein in cell-free system using the extract 失效在核糖体蛋白S12中具有突变的大肠杆菌细胞的提取物，以及使用提取物在无细胞系统中产生蛋白质的方法公开(公告)号：US08603774B2
公开(公告)日：2013-12-10
申请号：US11137395
申请日：2005-05-26
申请人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
发明人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
IPC分类号： C12P21/02
CPC分类号： C12P21/02
摘要： It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
摘要翻译： 旨在提供在无细胞系统中合成蛋白质的提取物，试剂盒和方法。 提取物的特征在于由在核糖体蛋白S12基因中具有突变的突变型大肠杆菌细胞制备。 在一个优选的实施方案中，突变是这样的突变，其赋予对大肠杆菌对链霉素的抗性或依赖性，从而可以提高核糖体上的mRNA密码子的阅读效率，从而提高蛋白质生产力。
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公开(公告)号：US20060008871A1
公开(公告)日：2006-01-12
申请号：US11137395
申请日：2005-05-26
申请人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
发明人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
CPC分类号： C12P21/02
摘要： It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
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4.发明申请EXTRACT OF E. COLI CELLS HAVING MUTATION IN RIBOSOMAL PROTEIN S12, AND METHOD FOR PRODUCING PROTEIN IN CELL-FREE SYSTEM USING THE EXTRACT 失效提取RIBOSOMAL蛋白S12中的突变的大肠杆菌细胞的提取，以及使用提取物在无细胞系统中生产蛋白质的方法公开(公告)号：US20110136204A1
公开(公告)日：2011-06-09
申请号：US12912724
申请日：2010-10-26
申请人： Namtip CHUMPOLKULWONG , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
发明人： Namtip CHUMPOLKULWONG , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
CPC分类号： C12P21/02
摘要： It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
摘要翻译： 旨在提供在无细胞系统中合成蛋白质的提取物，试剂盒和方法。 提取物的特征在于由在核糖体蛋白S12基因中具有突变的突变型大肠杆菌细胞制备。 在一个优选的实施方案中，突变是这样的突变，其赋予对大肠杆菌对链霉素的抗性或依赖性，从而可以提高核糖体上的mRNA密码子的阅读效率，从而提高蛋白质生产力。
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5.发明申请公开(公告)号：US20060134735A1
公开(公告)日：2006-06-22
申请号：US11282613
申请日：2005-11-21
申请人： Natsuko Matsuda , Takanori Kigawa , Namtip Chumpolkulwong , Chie Takemoto , Mikako Shirouzu , Akiko Tanaka , Shigeyuki Yokoyama
发明人： Natsuko Matsuda , Takanori Kigawa , Namtip Chumpolkulwong , Chie Takemoto , Mikako Shirouzu , Akiko Tanaka , Shigeyuki Yokoyama
IPC分类号： C12P21/06
摘要： It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The above-described cells are preferably bacterial cells, in particular, Escherichia coli cells. In the suppressed growth conditions as described above, the culture temperature is preferably from 20 to 32° C., more preferably 26° C. or higher and lower than 30° C.
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6.发明授权Extract of E. coli cells having mutation in ribosomal protein S12, and method for producing protein in cell-free system using the extract 失效在核糖体蛋白S12中具有突变的大肠杆菌细胞的提取物，以及使用提取物在无细胞系统中产生蛋白质的方法公开(公告)号：US08623626B2
公开(公告)日：2014-01-07
申请号：US12912724
申请日：2010-10-26
申请人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
发明人： Namtip Chumpolkulwong , Chie Hori , Shigeyuki Yokoyama , Mikako Shirouzu , Takanori Kigawa , Kozo Ochi , Takeshi Hosaka
CPC分类号： C12P21/02
摘要： It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
摘要翻译： 旨在提供在无细胞系统中合成蛋白质的提取物，试剂盒和方法。 提取物的特征在于由在核糖体蛋白S12基因中具有突变的突变型大肠杆菌细胞制备。 在一个优选的实施方案中，突变是这样的突变，其赋予对大肠杆菌对链霉素的抗性或依赖性，从而可以提高核糖体上的mRNA密码子的阅读效率，从而提高蛋白质生产力。
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7.发明申请PROCESS FOR PRODUCING EXTRACT FOR CELL-FREE PROTEIN SYNTHESIS AND CELL EXTRACT PRODUCED THEREBY 审中-公开生产无细胞蛋白质合成提取物的方法和生产的细胞提取物公开(公告)号：US20100168389A1
公开(公告)日：2010-07-01
申请号：US12565638
申请日：2009-09-23
申请人： Natsuko Matsuda , Takanori Kigawa , Namtip Chumpolkulwong , Chie Takemoto , Mikako Shirouzu , Akiko Tanaka , Shigeyuki Yokoyama
发明人： Natsuko Matsuda , Takanori Kigawa , Namtip Chumpolkulwong , Chie Takemoto , Mikako Shirouzu , Akiko Tanaka , Shigeyuki Yokoyama
IPC分类号： C07K14/00
摘要： It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The above-described cells are preferably bacterial cells, in particular, Escherichia coli cells. In the suppressed growth conditions as described above, the culture temperature is preferably from 20 to 32° C., more preferably 26° C. or higher and lower than 30° C.
摘要翻译： 旨在提供一种用于生产无细胞蛋白质合成提取物的方法，由此可以提高蛋白质的生产率和生产效率。 细胞在抑制生长条件下培养。 在培养物的固定相中，收集细胞，然后破碎。 上述细胞优选为细菌细胞，特别是大肠杆菌细胞。 在上述抑制生长条件下，培养温度优选为20〜32℃，更优选为26℃以上且低于30℃。
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