公开(公告)号：US10087423B2

公开(公告)日：2018-10-02

申请号：US13811093

申请日：2011-07-15

申请人： Engelbert Wehnes ,  Uwe Werner ,  Christian Leschke

发明人： Engelbert Wehnes ,  Uwe Werner ,  Christian Leschke

IPC分类号： C07H21/04 ,  C12N7/00 ,  A61K39/395 ,  A61K48/00 ,  C12N5/00 ,  C12N7/02 ,  C12M1/00

摘要： The present invention provides methods and systems for recovering an essentially cell-associated expression product from a host cell comprising (a) culturing said host cell under conditions that allow expression of said expression product; (b) collecting said host cell in/on a filter unit; (c) disrupting said host cell in/on the filter unit; and (d) separating said expression product from said disrupted host cell. The present invention also provides an expression product obtainable by the methods or systems, wherein the expression product is a poxvirus.

公开(公告)号：US20130183742A1

公开(公告)日：2013-07-18

申请号：US13811093

申请日：2011-07-15

申请人： Engelbert Wehnes ,  Uwe Werner ,  Christian Leschke

发明人： Engelbert Wehnes ,  Uwe Werner ,  Christian Leschke

IPC分类号： C12N7/02 ,  C12M1/00

CPC分类号： C12N7/02 ,  C12M47/02 ,  C12N7/00 ,  C12N2710/24151

摘要： The present invention provides a method for recovering an essentially cell-associated expression product from a host cell comprising (a) culturing said host cell under conditions that allow expression of said expression product; (b) collecting said host cell in/on a filter unit; (c) disrupting said host cell in/on the filter unit; and (d) separating said expression product from said disrupted host cell. Said host cell is preferably a vertebrate cell, more preferably an avian cell, which is preferably cultured in suspension. Furthermore, the present invention provides for the use of a filter unit characterized in that said filter unit is (i) suitable to retain a host cell which expresses an expression product; and (ii) suitable for elution of said expression product from the filter unit after cell disruption in/on said filter unit for recovering said expression product from said host cell as well as for a system for recovering an expression product from a host cell comprising said filter unit. The present invention also provides an expression product obtainable by said method, said expression product being preferably a virus, specifically a poxvirus, in particular selected from the group consisting of fowlpoxvirus, vaccinia virus and, more preferably, modified vaccinia virus Ankara, MVA.

摘要翻译： 本发明提供了从宿主细胞中回收基本上与细胞相关的表达产物的方法，包括（a）在允许所述表达产物表达的条件下培养所述宿主细胞; （b）在过滤器单元中/之上收集所述宿主细胞; （c）在所述过滤器单元中/之上破坏所述宿主细胞; 和（d）从所述破坏的宿主细胞中分离所述表达产物。 所述宿主细胞优选是脊椎动物细胞，更优选禽类细胞，其优选以悬浮培养。 此外，本发明提供了一种过滤器单元的使用，其特征在于所述过滤器单元是（i）适于保留表达表达产物的宿主细胞; 和（ii）适合于在所述过滤器单元中/之后的细胞破碎之后从所述过滤器单元洗脱所述表达产物，用于从所述宿主细胞回收所述表达产物，以及用于从包含所述宿主细胞的宿主细胞中回收表达产物的系统 过滤器单元 本发明还提供可通过所述方法获得的表达产物，所述表达产物优选病毒，特别是痘病毒，特别是选自禽痘病毒，痘苗病毒，更优选修饰的痘苗病毒安卡拉，MVA。