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公开(公告)号：US20130183742A1
公开(公告)日：2013-07-18
申请号：US13811093
申请日：2011-07-15
申请人： Engelbert Wehnes , Uwe Werner , Christian Leschke
发明人： Engelbert Wehnes , Uwe Werner , Christian Leschke
CPC分类号： C12N7/02 , C12M47/02 , C12N7/00 , C12N2710/24151
摘要： The present invention provides a method for recovering an essentially cell-associated expression product from a host cell comprising (a) culturing said host cell under conditions that allow expression of said expression product; (b) collecting said host cell in/on a filter unit; (c) disrupting said host cell in/on the filter unit; and (d) separating said expression product from said disrupted host cell. Said host cell is preferably a vertebrate cell, more preferably an avian cell, which is preferably cultured in suspension. Furthermore, the present invention provides for the use of a filter unit characterized in that said filter unit is (i) suitable to retain a host cell which expresses an expression product; and (ii) suitable for elution of said expression product from the filter unit after cell disruption in/on said filter unit for recovering said expression product from said host cell as well as for a system for recovering an expression product from a host cell comprising said filter unit. The present invention also provides an expression product obtainable by said method, said expression product being preferably a virus, specifically a poxvirus, in particular selected from the group consisting of fowlpoxvirus, vaccinia virus and, more preferably, modified vaccinia virus Ankara, MVA.
摘要翻译： 本发明提供了从宿主细胞中回收基本上与细胞相关的表达产物的方法，包括（a）在允许所述表达产物表达的条件下培养所述宿主细胞; （b）在过滤器单元中/之上收集所述宿主细胞; （c）在所述过滤器单元中/之上破坏所述宿主细胞; 和（d）从所述破坏的宿主细胞中分离所述表达产物。 所述宿主细胞优选是脊椎动物细胞，更优选禽类细胞，其优选以悬浮培养。 此外，本发明提供了一种过滤器单元的使用，其特征在于所述过滤器单元是（i）适于保留表达表达产物的宿主细胞; 和（ii）适合于在所述过滤器单元中/之后的细胞破碎之后从所述过滤器单元洗脱所述表达产物，用于从所述宿主细胞回收所述表达产物，以及用于从包含所述宿主细胞的宿主细胞中回收表达产物的系统 过滤器单元 本发明还提供可通过所述方法获得的表达产物，所述表达产物优选病毒，特别是痘病毒，特别是选自禽痘病毒，痘苗病毒，更优选修饰的痘苗病毒安卡拉，MVA。
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公开(公告)号：US10087423B2
公开(公告)日：2018-10-02
申请号：US13811093
申请日：2011-07-15
申请人： Engelbert Wehnes , Uwe Werner , Christian Leschke
发明人： Engelbert Wehnes , Uwe Werner , Christian Leschke
摘要： The present invention provides methods and systems for recovering an essentially cell-associated expression product from a host cell comprising (a) culturing said host cell under conditions that allow expression of said expression product; (b) collecting said host cell in/on a filter unit; (c) disrupting said host cell in/on the filter unit; and (d) separating said expression product from said disrupted host cell. The present invention also provides an expression product obtainable by the methods or systems, wherein the expression product is a poxvirus.
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公开(公告)号：US20140051035A1
公开(公告)日：2014-02-20
申请号：US14004329
申请日：2012-02-02
申请人： Marco Stoffel , Uwe Werner
发明人： Marco Stoffel , Uwe Werner
摘要： A description is given of a sonotrode (18) for connection to an ultrasonic vibration transducer (2, 3) comprising a connecting portion (4), which extends along a first axis (16) and is attached by a first end (42) to the vibration transducer (2, 3) or is connected thereto, wherein the connecting portion (4) is made to vibrate almost exclusively along the first axis (16) by the ultrasonic vibration transducer (2, 3). Said sonotrode is characterized in that, at the free end (43) of the connecting portion (4), opposite from the first end (42), both a substantially cylindrical, first sleeve (5), which extends along a second axis (8), and a substantially cylindrical, second sleeve (6), which extends along a third axis (17), are arranged in a common crossing region (36), wherein the first axis (16) forms a first angle (γ) in the range of 100-140° with the second axis (8), and wherein the first axis (16) forms a second angle (β) in the range of 100-140° with the third axis (17), wherein the three axes (8, 16, 17) are arranged substantially in one sonotrode plane (41). Moreover, corresponding sonotrode tools and uses of such sonotrode tools are described.
摘要翻译： 给出了用于连接到超声波振动换能器（2,3）的超声波发生器（18）的描述，该超声波振荡器包括连接部分（4），该连接部分沿着第一轴线（16）延伸并且通过第一端部（42）附接到 所述振动传感器（2,3）或与其连接，其中所述连接部分（4）通过所述超声波振动传感器（2,3）沿所述第一轴线（16）几乎完全振动。 所述超声波发生器的特征在于，在与第一端（42）相对的连接部分（4）的自由端（43）处，大致圆柱形的第一套筒（5）沿着第二轴线（8 ）和沿着第三轴线（17）延伸的大致圆柱形的第二套筒（6）布置在公共交叉区域（36）中，其中第一轴线（16）在第二轴线（16）中形成第一角度（γ） 与第二轴线（8）的范围为100-140°，并且其中第一轴线（16）与第三轴线（17）形成在100-140°范围内的第二角度（β），其中三个轴线 8,16,17）基本上布置在一个超声波平面（41）中。 此外，描述了相应的超声波工具和这种超声波工具的用途。
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公开(公告)号：US20130128560A1
公开(公告)日：2013-05-23
申请号：US13699808
申请日：2011-04-05
申请人： Uwe Werner , Georgios Margaritis
发明人： Uwe Werner , Georgios Margaritis
IPC分类号： F21V33/00
CPC分类号： B25H3/02 , B25H3/023 , B65D85/00 , F21V33/00 , F21V33/0084
摘要： A handheld tool carrying case having a carrying case base and a carrying case cover and at least one electronic system. The carrying case base or the carrying case cover has at least one battery interface and one lighting device, which are at least partly fixedly connected to the carrying case base or fixedly connected to the carrying case cover.
摘要翻译： 一种手持式工具手提箱，具有便携箱基座和手提箱盖以及至少一个电子系统。 手提箱基座或手提箱盖具有至少一个电池接口和一个照明装置，其至少部分地固定地连接到便携式基座或固定地连接到手提箱盖。
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5.发明授权Control device and control method for fixing an information output order for several information sources, especially audio sources 失效用于固定多个信息源，特别是音频源的信息输出顺序的控制装置和控制方法公开(公告)号：US07558635B1
公开(公告)日：2009-07-07
申请号：US10088968
申请日：2000-09-05
申请人： Steffen Thiel , Ralf Hahn , Ute Gappa , Uwe Werner , Peter Lutz
发明人： Steffen Thiel , Ralf Hahn , Ute Gappa , Uwe Werner , Peter Lutz
CPC分类号： G08G1/096716 , G01C21/3629 , G08G1/09675 , G08G1/096775
摘要： A control device and a corresponding control method are provided for establishing an information-output ranking of a plurality of information sources, in particular audio sources. In order to output the information of the information sources to a common information-output device, the information-output ranking is established in pairs for the audio sources which is in the form of an audio-output matrix having a nonlinear order with respect to the matrix elements, and wherein each matrix element of the information-output matrix is used to determine the priority of a corresponding information source with respect to another information source. A conflict among competing information outputs is solved in this manner.
摘要翻译： 提供了一种控制装置和相应的控制方法，用于建立多个信息源，特别是音频源的信息输出排序。 为了将信息源的信息输出到公共信息输出装置，信息输出排列成立为音频源，其音频源的形式为具有相对于 矩阵元素，并且其中信息输出矩阵的每个矩阵元素用于确定相对于另一信息源的对应信息源的优先级。 以这种方式解决竞争信息输出之间的冲突。
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6.发明授权Machine for and method of chamfering of edges of plate-shaped workpieces, particularly glass disks 失效板状工件，特别是玻璃盘的边缘倒角的机器和倒角方法
公开(公告)号：US4594814A
公开(公告)日：1986-06-17
申请号：US763422
申请日：1985-08-07
申请人： Egon Olszewski , Klaus-Dieter Schaper , Uwe Werner
发明人： Egon Olszewski , Klaus-Dieter Schaper , Uwe Werner
CPC分类号： B24B9/10
摘要： Chamfering of one or two edges of a glass disk is performed by one or two peripheral grinding wheels which move relative to the glass disk and rotate about axes of rotation which are parallel to the edges to be chamfered.
摘要翻译： 玻璃盘的一个或两个边缘的倒角由相对于玻璃盘移动的一个或两个周边砂轮执行，并且围绕平行于待倒角边缘的旋转轴旋转。
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公开(公告)号：US10539317B2
公开(公告)日：2020-01-21
申请号：US13699795
申请日：2011-04-05
申请人： Uwe Werner , Georgios Margaritis
发明人： Uwe Werner , Georgios Margaritis
摘要： A handheld tool carrying case having a carrying case base and a carrying case cover and at least one electronic system. The carrying case base is connected to the carrying case cover in a detachable fashion.
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公开(公告)号：US08490943B2
公开(公告)日：2013-07-23
申请号：US12929739
申请日：2011-02-11
申请人： Daniel Bühler , Jörg Evertz , Martin Sprecher , Uwe Werner
发明人： Daniel Bühler , Jörg Evertz , Martin Sprecher , Uwe Werner
IPC分类号： F16K31/44
CPC分类号： F16K31/055 , F16K31/048
摘要： A valve of a heating, ventilation and/or air conditioning system includes an actuator with a housing and a shaft which is arranged at least partially in the housing and which can be coupled directly or indirectly to the valve such that the valve can be adjusted during a rotation of the shaft. A hand-operated element can be coupled to the shaft via an overload clutch arranged between the hand-operated element and the shaft which limits a torque which is transmitted onto the shaft by the hand-operated element to a predetermined maximum value. The overload clutch has a coupling element and a replaceable spring element which presses the coupling element against the shaft such that the maximum value for the transmittable torque is dependent on the replaceable spring element.
摘要翻译： 加热，通风和/或空调系统的阀包括具有壳体和轴的致动器，该致动器至少部分地布置在壳体中并且可以直接或间接地连接到阀，使得阀可以在 轴的旋转。 手动元件可以通过设置在手动操作元件和轴之间的过载离合器联接到轴上，该过载离合器将由手动元件传递到轴上的转矩限制到预定的最大值。 过载离合器具有联接元件和可更换的弹簧元件，其将联接元件压靠在轴上，使得可传递扭矩的最大值取决于可更换的弹簧元件。
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公开(公告)号：USD664659S1
公开(公告)日：2012-07-31
申请号：US29369062
申请日：2010-09-01
申请人： Matthias Günthart , Daniel Irányi , Falko Schlottig , Nico Spinelli , Uwe Werner
设计人： Matthias Günthart , Daniel Irányi , Falko Schlottig , Nico Spinelli , Uwe Werner
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10.发明授权公开(公告)号：US08086406B2
公开(公告)日：2011-12-27
申请号：US10590612
申请日：2005-01-05
申请人： Frank Ewerhart , Uwe Werner , Heiko Freienstein , Jan Tilp , Joachim Selinger , Soenke Carstens-Behrens , Markus Hagemann
发明人： Frank Ewerhart , Uwe Werner , Heiko Freienstein , Jan Tilp , Joachim Selinger , Soenke Carstens-Behrens , Markus Hagemann
IPC分类号： G08G1/16
CPC分类号： B62D15/0265 , B60T7/22 , B60T2201/02 , B60T2201/022 , B60W30/02 , B60W30/09 , B60W40/09 , B60W50/08 , B60W50/12 , B62D6/001 , G08G1/161
摘要： In order to enhance a safety system, in particular an accident avoidance system for a means of transportation, in particular a motor vehicle, having at least one steering system and at least one brake system in such a way that an avoidance maneuver initiated by the operator of the means of transportation when approaching an obstacle is supported with respect to both the device and the method, thus preventing an accident through collision, the evaluation unit determines at least one driving variation, in particular at least one avoidance trajectory and/or at least one automatic emergency braking action from the data and information and when or after the operator of the means of transportation initiates a driving maneuver, in particular an avoidance maneuver or an emergency braking maneuver, the safety system, in particular the evaluation unit specifies, supports and/or suggests this driving maneuver in an optimized form, in particular in the form of an optimal avoidance trajectory or in the form of an automatic emergency braking.
摘要翻译： 为了增强安全系统，特别是用于运输工具，特别是机动车辆的事故避免系统，其具有至少一个转向系统和至少一个制动系统，使得由操作者启动的回避机动 在接近障碍物时的运输工具相对于装置和方法被支撑，从而防止碰撞中的事故，评估单元确定至少一个行驶变化，特别是至少一个回避轨迹和/或至少 数据和信息的一个自动紧急制动动作，当运输工具的操作人员或之后启动驾驶操作，特别是回避机动或紧急制动操作时，安全系统，特别是评估单元指定，支持和 /或者以优化的形式提出这种驾驶操纵，特别是以最佳回避轨迹的形式 自动紧急制动的形式。
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