公开(公告)号：US20120100559A1

公开(公告)日：2012-04-26

申请号：US13266775

申请日：2010-04-26

申请人： Stefan Hell ,  Vladimir N. Belov ,  Kirill Kolmakov ,  Volker Westphal ,  Marcel Lauterbach ,  Stefan Jakobs ,  Christian Wurm ,  Christian Eggeling ,  Christian Ringemann

发明人： Stefan Hell ,  Vladimir N. Belov ,  Kirill Kolmakov ,  Volker Westphal ,  Marcel Lauterbach ,  Stefan Jakobs ,  Christian Wurm ,  Christian Eggeling ,  Christian Ringemann

IPC分类号： G01N33/567 ,  C07D491/22 ,  C07H21/00 ,  C12Q1/02 ,  C07H1/00

CPC分类号： C07D491/22 ,  C09B11/24 ,  G01N33/582

摘要： The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy. The partially deuterated analogues are useful as molecular mass distribution tags in mass spectroscopic applications. wherein R1=an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group; R2═H, an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group, or an unsubstituted or substituted aryl group or heteroaryl group, or any combination of such groups; X═CH2, C═O, C═NORa, C═NNRaNRb, CH(ORa), O, S, SO, SO2, or any other derivatives of these groups, with Ra and Rb independently being H or an organic residue, in particular an unsubstituted or substituted (cyclo) alkyl group or heterocycloalkyl group, an unsubstituted or substituted aryl group or heteroaryl group; Z=a negatively charged group with 1, 2, 3, 4 or 5 charges per anion.

摘要翻译： 本发明涉及通用结构式I或II的新型和改进的光稳定罗丹明染料及其作为荧光标记物的用途。 用于免疫染色和光谱和微观应用，特别是在常规和受激发射耗尽（STED）显微镜和荧光相关光谱学中。 部分氘代类似物可用作质谱分析应用中的分子量分布标签。 其中R1 =未取代或取代的烷基，包括环烷基或杂环烷基; R 2 = H，未取代或取代的烷基，包括环烷基或杂环烷基，或未取代或取代的芳基或杂芳基，或这些基团的任何组合; X = CH 2，C = O，C = NOR a，C = NR N R a R b，CH（OR a），O，S，SO，SO 2或这些基团的任何其它衍生物，其中Ra和Rb独立地为H或有机残基 特别是未取代或取代的（环）烷基或杂环烷基，未取代或取代的芳基或杂芳基; Z =带负电荷的基团，每个阴离子具有1,2,3,4或5个电荷。

公开(公告)号：US08580579B2

公开(公告)日：2013-11-12

申请号：US13266775

申请日：2010-04-26

申请人： Stefan Hell ,  Vladimir N. Belov ,  Kirill Kolmakov ,  Volker Westphal ,  Marcel Lauterbach ,  Stefan Jakobs ,  Christian Wurm ,  Christian Eggeling ,  Christian Ringemann

发明人： Stefan Hell ,  Vladimir N. Belov ,  Kirill Kolmakov ,  Volker Westphal ,  Marcel Lauterbach ,  Stefan Jakobs ,  Christian Wurm ,  Christian Eggeling ,  Christian Ringemann

IPC分类号： G01N33/533 ,  G01N33/532 ,  G01N21/76

CPC分类号： C07D491/22 ,  C09B11/24 ,  G01N33/582

摘要： The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy. The partially deuterated analogues are useful as molecular mass distribution tags in mass spectroscopic applications, wherein R1=an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group; R2=H, an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group, or an unsubstituted or substituted aryl group or heteroaryl group, or any combination of such groups; X=CH2, C═O, C═NORa, C═NNRaNRb, CH(ORa), O, S, SO, SO2, or any other derivatives of these groups, with Ra and Rb independently being H or an organic residue, in particular an unsubstituted or substituted (cyclo)alkyl group or heterocycloalkyl group, an unsubstituted or substituted aryl group or heteroaryl group; Z=a negatively charged group with 1, 2, 3, 4 or 5 charges per anion.

摘要翻译： 本发明涉及通用结构式I或II的新型和改进的光稳定罗丹明染料及其作为荧光标记物的用途。 用于免疫染色和光谱和微观应用，特别是在常规和受激发射耗尽（STED）显微镜和荧光相关光谱学中。 部分氘代类似物可用作质谱应用中的分子量分布标签，其中R1 =未取代或取代的烷基，包括环烷基或杂环烷基; R 2 = H，未取代或取代的烷基，包括环烷基或杂环烷基，或未取代或取代的芳基或杂芳基，或这些基团的任何组合; X = CH2，C = O，C = NORa，C = NNRaNRb，CH（ORa），O，S，SO，SO2或这些基团的任何其他衍生物，其中Ra和Rb独立地为H或有机残基， 特别是未取代或取代的（环）烷基或杂环烷基，未取代或取代的芳基或杂芳基; Z =带负电荷的基团，每个阴离子具有1,2,3,4或5个电荷。