公开(公告)号：US20120100559A1

公开(公告)日：2012-04-26

申请号：US13266775

申请日：2010-04-26

申请人： Stefan Hell ,  Vladimir N. Belov ,  Kirill Kolmakov ,  Volker Westphal ,  Marcel Lauterbach ,  Stefan Jakobs ,  Christian Wurm ,  Christian Eggeling ,  Christian Ringemann

发明人： Stefan Hell ,  Vladimir N. Belov ,  Kirill Kolmakov ,  Volker Westphal ,  Marcel Lauterbach ,  Stefan Jakobs ,  Christian Wurm ,  Christian Eggeling ,  Christian Ringemann

IPC分类号： G01N33/567 ,  C07D491/22 ,  C07H21/00 ,  C12Q1/02 ,  C07H1/00

CPC分类号： C07D491/22 ,  C09B11/24 ,  G01N33/582

摘要： The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy. The partially deuterated analogues are useful as molecular mass distribution tags in mass spectroscopic applications. wherein R1=an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group; R2═H, an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group, or an unsubstituted or substituted aryl group or heteroaryl group, or any combination of such groups; X═CH2, C═O, C═NORa, C═NNRaNRb, CH(ORa), O, S, SO, SO2, or any other derivatives of these groups, with Ra and Rb independently being H or an organic residue, in particular an unsubstituted or substituted (cyclo) alkyl group or heterocycloalkyl group, an unsubstituted or substituted aryl group or heteroaryl group; Z=a negatively charged group with 1, 2, 3, 4 or 5 charges per anion.

摘要翻译： 本发明涉及通用结构式I或II的新型和改进的光稳定罗丹明染料及其作为荧光标记物的用途。 用于免疫染色和光谱和微观应用，特别是在常规和受激发射耗尽（STED）显微镜和荧光相关光谱学中。 部分氘代类似物可用作质谱分析应用中的分子量分布标签。 其中R1 =未取代或取代的烷基，包括环烷基或杂环烷基; R 2 = H，未取代或取代的烷基，包括环烷基或杂环烷基，或未取代或取代的芳基或杂芳基，或这些基团的任何组合; X = CH 2，C = O，C = NOR a，C = NR N R a R b，CH（OR a），O，S，SO，SO 2或这些基团的任何其它衍生物，其中Ra和Rb独立地为H或有机残基 特别是未取代或取代的（环）烷基或杂环烷基，未取代或取代的芳基或杂芳基; Z =带负电荷的基团，每个阴离子具有1,2,3,4或5个电荷。

公开(公告)号：US08580579B2

公开(公告)日：2013-11-12

申请号：US13266775

申请日：2010-04-26

申请人： Stefan Hell ,  Vladimir N. Belov ,  Kirill Kolmakov ,  Volker Westphal ,  Marcel Lauterbach ,  Stefan Jakobs ,  Christian Wurm ,  Christian Eggeling ,  Christian Ringemann

发明人： Stefan Hell ,  Vladimir N. Belov ,  Kirill Kolmakov ,  Volker Westphal ,  Marcel Lauterbach ,  Stefan Jakobs ,  Christian Wurm ,  Christian Eggeling ,  Christian Ringemann

IPC分类号： G01N33/533 ,  G01N33/532 ,  G01N21/76

CPC分类号： C07D491/22 ,  C09B11/24 ,  G01N33/582

摘要： The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy. The partially deuterated analogues are useful as molecular mass distribution tags in mass spectroscopic applications, wherein R1=an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group; R2=H, an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group, or an unsubstituted or substituted aryl group or heteroaryl group, or any combination of such groups; X=CH2, C═O, C═NORa, C═NNRaNRb, CH(ORa), O, S, SO, SO2, or any other derivatives of these groups, with Ra and Rb independently being H or an organic residue, in particular an unsubstituted or substituted (cyclo)alkyl group or heterocycloalkyl group, an unsubstituted or substituted aryl group or heteroaryl group; Z=a negatively charged group with 1, 2, 3, 4 or 5 charges per anion.

摘要翻译： 本发明涉及通用结构式I或II的新型和改进的光稳定罗丹明染料及其作为荧光标记物的用途。 用于免疫染色和光谱和微观应用，特别是在常规和受激发射耗尽（STED）显微镜和荧光相关光谱学中。 部分氘代类似物可用作质谱应用中的分子量分布标签，其中R1 =未取代或取代的烷基，包括环烷基或杂环烷基; R 2 = H，未取代或取代的烷基，包括环烷基或杂环烷基，或未取代或取代的芳基或杂芳基，或这些基团的任何组合; X = CH2，C = O，C = NORa，C = NNRaNRb，CH（ORa），O，S，SO，SO2或这些基团的任何其他衍生物，其中Ra和Rb独立地为H或有机残基， 特别是未取代或取代的（环）烷基或杂环烷基，未取代或取代的芳基或杂芳基; Z =带负电荷的基团，每个阴离子具有1,2,3,4或5个电荷。

公开(公告)号：US20080018891A1

公开(公告)日：2008-01-24

申请号：US11856887

申请日：2007-09-18

申请人： Stefan Hell ,  Volker Westphal ,  Norbert Quaas

发明人： Stefan Hell ,  Volker Westphal ,  Norbert Quaas

IPC分类号： G01J3/30

CPC分类号： G01N21/6458 ,  G01N21/6489 ,  G01N21/91 ,  G01N21/956 ,  G02B21/0076 ,  G03F7/105 ,  G03F7/70616 ,  G03F7/70675

摘要： A method of producing spatial fine structures comprises the steps of: selecting a luminophore from the group of luminophores displaying two different states, one of the two states being an active state in which luminescence light is obtainable from the luminophore, the other of the two states being an inactive state in which no luminescence light is obtainable from the luminophore, and the luminophore being reversibly, but essentially completely, transferable out the one state into the other state by means of an optical signal; adding the luminophore to a material; forming a spatial fine structure of the material; and fluorescence-microscopically examining whether the desired fine structure is present. The step of fluorescence-microscopically examining comprises the sub-steps of: outside measuring points of interest, transferring the luminophore into the other state with the optical signal, the luminophore being essentially completely transferred into the inactive state outside the measuring points, and measuring luminescence light only emitted by the luminophore in the active state, to even resolve lines of the fine structure at a distance of less than 100 nm.

摘要翻译： 一种产生空间精细结构的方法包括以下步骤：从显示两种不同状态的发光体组中选择发光体，两种状态中的一种状态是可从发光体获得发光的活性状态，两种状态中的另一种状态 是其中从发光体不能获得发光的无活性状态，并且发光体是可逆的，但基本上完全可通过光信号转移到一个状态到另一状态; 将发光体添加到材料中; 形成材料的空间精细结构; 并进行荧光显微镜检查是否存在所需的精细结构。 荧光显微镜检查的步骤包括以下子步骤：在感兴趣的测量点之外，用光信号将发光体转移到另一状态，发光体基本上完全转移到测量点外的无效状态，并测量发光 发光体仅在活性状态下发光，甚至在小于100nm的距离处解决精细结构的线。

公开(公告)号：US07538893B2

公开(公告)日：2009-05-26

申请号：US11861405

申请日：2007-09-26

申请人： Stefan Hell ,  Volker Westphal ,  Norbert Quaas

发明人： Stefan Hell ,  Volker Westphal ,  Norbert Quaas

IPC分类号： G01B11/24

CPC分类号： G01N21/643 ,  G01N21/6458

摘要： A method of microscopically examining a spatial fine structure comprises the steps of selecting a luminophore from the group of luminophores which have two physical states, the two states differing from each other with regard to the luminescence properties displayed by the luminophore, and which are reversibly, but essentially completely transferable out of one into the other state of their two states by means of an optical signal; overlaying a surface of the spatial fine structure with the luminophore; and determining the profile of the surface overlaid with the luminophore. The step of determining the profile of the surface comprises the sub-steps of transferring the luminophore by means of the optical signal out of the one into the other of its two states outside a presently observed measurement point, measuring luminescence light emitted by the luminophore, and repeating the sub-steps of transferring and measuring for further measurement points distributed over the surface.

摘要翻译： 显微镜检查空间精细结构的方法包括以下步骤：从具有两个物理状态的发光体组中选择发光体，两个状态相对于由发光体显示的发光特性彼此不同，并且是可逆的， 但是通过光信号基本上完全可以从一个传输到它们的两个状态的另一状态; 用发光体覆盖空间精细结构的表面; 并确定覆盖有发光体的表面的轮廓。 确定表面轮廓的步骤包括将发光体通过一个光信号转移到目前观察到的测量点之外的两个状态中的另一个状态的子步骤，测量发光体发出的发光， 并重复传送和测量分布在表面上的其他测量点的子步骤。

公开(公告)号：US07764369B2

公开(公告)日：2010-07-27

申请号：US11856887

申请日：2007-09-18

申请人： Stefan Hell ,  Volker Westphal ,  Norbert Quaas

发明人： Stefan Hell ,  Volker Westphal ,  Norbert Quaas

IPC分类号： G01N21/00 ,  G01J3/30

CPC分类号： G01N21/6458 ,  G01N21/6489 ,  G01N21/91 ,  G01N21/956 ,  G02B21/0076 ,  G03F7/105 ,  G03F7/70616 ,  G03F7/70675

摘要： A method of producing spatial fine structures comprises the steps of: selecting a luminophore from the group of luminophores displaying two different states, one of the two states being an active state in which luminescence light is obtainable from the luminophore, the other of the two states being an inactive state in which no luminescence light is obtainable from the luminophore, and the luminophore being reversibly, but essentially completely, transferable out the one state into the other state by means of an optical signal; adding the luminophore to a material; forming a spatial fine structure of the material; and fluorescence-microscopically examining whether the desired fine structure is present. The step of fluorescence-microscopically examining comprises the sub-steps of: outside measuring points of interest, transferring the luminophore into the other state with the optical signal, the luminophore being essentially completely transferred into the inactive state outside the measuring points, and measuring luminescence light only emitted by the luminophore in the active state, to even resolve lines of the fine structure at a distance of less than 100 nm.

摘要翻译： 一种产生空间精细结构的方法包括以下步骤：从显示两种不同状态的发光体组中选择发光体，两种状态中的一种状态是可从发光体获得发光的活性状态，两种状态中的另一种状态 是其中从发光体不能获得发光的无活性状态，并且发光体是可逆的，但基本上完全可通过光信号转移到一个状态到另一状态; 将发光体添加到材料中; 形成材料的空间精细结构; 并进行荧光显微镜检查是否存在所需的精细结构。 荧光显微镜检查的步骤包括以下子步骤：在感兴趣的测量点之外，用光信号将发光体转移到另一状态，发光体基本上完全转移到测量点外的无效状态，并测量发光 发光体仅在活性状态下发光，甚至在小于100nm的距离处解决精细结构的线。

公开(公告)号：US08755116B2

公开(公告)日：2014-06-17

申请号：US12628408

申请日：2009-12-01

申请人： Lars Kastrup ,  Volker Westphal

发明人： Lars Kastrup ,  Volker Westphal

IPC分类号： G02B5/30 ,  G02B27/00 ,  G02B27/28

CPC分类号： G02B21/0076 ,  G01N21/6458

摘要： In an optical assembly having a light source which provides two optically different light components with essentially planar wavefronts on an optical axis, wherein the light components differ at least in their wavelength; in the case of an objective lens which projects the two optically different light components into a projection space; and in the case of an optical component which is arranged on the optical axis and has an plane through which the wavefronts of the two light components pass and in which at least two different areas of the optical component with different dispersion behaviors n(λ) abut against one another in the lateral direction with respect to the optical axis; the optical component causes phase shifts of the wavefronts of the two light components, wherein the phase shift of the wavefronts of the one light component differs by at least one quarter of the wavelength of that light component between the two different areas, and wherein the phase shift of the wavefronts of the other light component does not differ between the two different areas, such that an intensity distribution of the one light component in the projection space by interference with itself differs from an intensity distribution of the other light component in the projection space.

摘要翻译： 在具有光源的光学组件中，所述光源在光轴上提供具有基本上平面的波前的两个光学不同的光分量，其中所述光分量至少在其波长上不同; 在将两个光学不同的光分量投影到投影空间中的物镜的情况下; 并且在配置在光轴上并具有两个光分量的波前通过的平面的光学部件的情况下，并且其中具有不同分散行为n（λ）的光学部件的至少两个不同区域邻接 在相对于光轴的横向上彼此相对; 光学部件引起两个光分量的波前的相移，其中一个光分量的波前的相移相差两个不同区域之间的该光分量的波长的至少四分之一，并且其中相位 另外的光分量的波前的偏移在两个不同的区域之间没有差别，使得投影空间中的一个光分量的强度分布与其自身的干涉不同于投射空间中的另一个光分量的强度分布 。

公开(公告)号：US07061622B2

公开(公告)日：2006-06-13

申请号：US10213326

申请日：2002-08-05

申请人： Andrew M. Rollins ,  Joseph A. Izatt ,  Volker Westphal ,  Siavash Yazdanfar

发明人： Andrew M. Rollins ,  Joseph A. Izatt ,  Volker Westphal ,  Siavash Yazdanfar

IPC分类号： G01B9/02

CPC分类号： G01N21/4795 ,  A61B5/0066 ,  G01B11/2441

摘要： An optical coherence tomography (OCT) system including an interferometer provides illuminating light along a first optical path to a sample and an optical delay line and collects light from the sample along a second optical path remitted at several scattering angles to a detector. In one embodiment, illuminating light is directed along a number of incident light paths through a focusing lens to a sample. The light paths and focusing lens are related to the sample and to both the incident light source and the detector. In another embodiment, a focusing system directs light to a location in the sample. A transmission grating or acousto-optic modulator directs light from the sample at an angle representative of the wavelength of the incident light on the transmission grating or acousto-optic modulator.

公开(公告)号：US07072047B2

公开(公告)日：2006-07-04

申请号：US10619574

申请日：2003-07-14

申请人： Volker Westphal ,  Andrew M. Rollins ,  Joseph A. Izatt

发明人： Volker Westphal ,  Andrew M. Rollins ,  Joseph A. Izatt

IPC分类号： G01B9/02

CPC分类号： G01B9/02091 ,  A61B3/102 ,  G01B9/02083

摘要： A method of correcting optical coherence tomography (OCT) image data obtained from a layered media sample includes identifying at least one interface from the obtained OCT data and correcting the OCT data for distortion at the at least one interface. The OCT image data can be corrected for extrinsic distortions, such as those caused by scan geometry, as well as, intrinsic distortions, such as those caused by refraction at each interface.

摘要翻译： 校正从分层介质样本获得的光学相干断层摄影（OCT）图像数据的方法包括从所获得的OCT数据识别至少一个接口，并且在所述至少一个界面处校正OCT数据以进行失真。 OCT图像数据可以被校正为外部失真，例如由扫描几何引起的失真，以及固有失真，例如由每个界面处的折射造成的。

公开(公告)号：US07006232B2

公开(公告)日：2006-02-28

申请号：US10408745

申请日：2003-04-07

申请人： Andrew M. Rollins ,  Joseph A. Izatt ,  Volker Westphal ,  Cameron J. Pedersen ,  Siavash Yazdanfar

发明人： Andrew M. Rollins ,  Joseph A. Izatt ,  Volker Westphal ,  Cameron J. Pedersen ,  Siavash Yazdanfar

IPC分类号： G01B9/02

CPC分类号： G01B9/02045 ,  G01B9/02007 ,  G01B9/02076 ,  G01B9/02083 ,  G01B9/02091 ,  G01B2290/45

摘要： A phase-referenced Doppler optical coherence tomography (OCT) system includes a low-coherence optical radiation source and a reference source co-propagated to a sample arm and a reference arm. The low-coherence and reference optical radiation reflected from the reference and arms is detected by a pair of detectors, yielding OCT and reference interferometric data output signals. The reference interferometric data output signal can be used to correct the OCT interferometric to yield velocity-indicating images that are free from defects due to sample motion and/or interferometer jitter.