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    Method Of Excising A Nucleic Acid Sequence From A Plant Genome
    3.
    发明申请
    Method Of Excising A Nucleic Acid Sequence From A Plant Genome 审中-公开
    从植物基因组中切除核酸序列的方法

    公开(公告)号：US20100162428A1

    公开(公告)日：2010-06-24

    申请号：US12600902

    申请日：2008-05-30

    申请人： Jeffrey A. Brown ,  Fang-Ming Lai ,  Christina E. Roche ,  Hee-Sook Song

    发明人： Jeffrey A. Brown ,  Fang-Ming Lai ,  Christina E. Roche ,  Hee-Sook Song

    IPC分类号： A01H1/00 ,  A01H5/00

    CPC分类号： C12N15/8201 ,  C12N9/22

    摘要： The present invention relates to a method for excising a nucleic acid sequence from the genome of a plant or a plant cell. This method is based on the steps of transforming a plant cell with a construct encoding a DNA double strand break inducing enzyme (DSBI), generating a transgenic plant line, performing a transient assay to analyze the functionality of the transgenic enzyme, crossing the plant line with a line containing a nucleic acid sequence to be excised and performing an immature embryo conversion or a tissue culture regeneration through callus formation. The method can also be reversed, which means that a plant cell is transformed with a construct encoding a nucleic acid sequence to be excised, and the crossing is performed with a plant line containing a DSBI. As an alternative to the crossing step, a re-transformation of a transgenic plant line with a second construct can also be performed. The invention is also directed to a plant obtained by this method, or progeny, propagation material, part, tissue, cell or cell culture, derived from such a plant. Finally, the invention relates to the use of a plant or progeny, propagation material, part, tissue, cell or cell culture, derived from this method, as aliment, fodder or seeds or for the production of pharmaceuticals or chemicals.

    摘要翻译： 本发明涉及从植物或植物细胞的基因组中切除核酸序列的方法。 该方法基于用编码DNA双链断裂诱导酶（DSBI）的构建体转化植物细胞的步骤，产生转基因植物系，进行瞬时测定以分析转基因酶的功能，穿过植物系 含有要切除的核酸序列的线，并通过愈伤组织形成进行未成熟胚转化或组织培养再生。 该方法也可以相反，这意味着用编码待切除的核酸序列的构建体转化植物细胞，并且用含有DSBI的植物系进行杂交。 作为交叉步骤的替代方案，也可以进行具有第二构建体的转基因植物系的再转化。 本发明还涉及通过该方法获得的植物，或从这种植物衍生的后代，繁殖材料，部分，组织，细胞或细胞培养物。 最后，本发明涉及来源于该方法的植物或后代，繁殖材料，部分，组织，细胞或细胞培养物作为营养物，饲料或种子或用于生产药物或化学品的用途。