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1.发明授权Identification and characterization of a novel alpha-amylase from maize endosperm 失效来自玉米胚乳的新型α-淀粉酶的鉴定和表征公开(公告)号：US07270988B2
公开(公告)日：2007-09-18
申请号：US10952551
申请日：2004-09-27
CPC分类号： C12N9/2422 , C12N15/8245 , C12P19/14
摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.
摘要翻译： SHE，在玉米胚乳（Zea mays）中活性的淀粉水解酶，以及编码SHE的cDNA序列。 天然纯化的SHE的特异性通常与以前已知的α-淀粉酶相似。 然而，SHE对支链淀粉的活性导致与其它α-淀粉酶明显不同的水解产物。 SHE及其在其他植物如水稻，拟南芥，苹果和马铃薯中的同源等同物可用于淀粉加工中，用于产生不同的，例如较大尺寸的工业用α限制糊精，与之前已知的 α-淀粉酶或其他淀粉水解酶。 此外，在转基因玉米植物或其他转基因生物（包括细菌，酵母和其他植物物种）中修饰该酶的表达可用于产生新的淀粉形式或改变的淀粉代谢。
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2.发明申请Identification and characterization of a novel alpha-amylase from maize endosperm 审中-公开来自玉米胚乳的新型α-淀粉酶的鉴定和表征公开(公告)号：US20090197323A1
公开(公告)日：2009-08-06
申请号：US12317838
申请日：2008-12-30
IPC分类号： C08B30/00
CPC分类号： C12N9/2422 , C12N15/8245 , C12P19/14
摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.
摘要翻译： SHE，在玉米胚乳（Zea mays）中活性的淀粉水解酶，以及编码SHE的cDNA序列。 天然纯化的SHE的特异性通常与以前已知的α-淀粉酶相似。 然而，SHE对支链淀粉的活性导致与其它α-淀粉酶明显不同的水解产物。 SHE及其在其他植物如水稻，拟南芥，苹果和马铃薯中的同源等同物可用于淀粉加工中，用于产生不同的，例如较大尺寸的工业用α限制糊精，与之前已知的 α-淀粉酶或其他淀粉水解酶。 此外，在转基因玉米植物或其他转基因生物（包括细菌，酵母和其他植物物种）中修饰该酶的表达可用于产生新的淀粉形式或改变的淀粉代谢。
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公开(公告)号：US07495152B2
公开(公告)日：2009-02-24
申请号：US11891589
申请日：2007-08-10
CPC分类号： C12N9/2422 , C12N15/8245 , C12P19/14
摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.
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4.发明申请Identification and characterization of a novel alpha-amylase from maize endosperm 失效来自玉米胚乳的新型α-淀粉酶的鉴定和表征公开(公告)号：US20080134363A1
公开(公告)日：2008-06-05
申请号：US11891589
申请日：2007-08-10
CPC分类号： C12N9/2422 , C12N15/8245 , C12P19/14
摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.
摘要翻译： SHE，在玉米胚乳（Zea mays）中活性的淀粉水解酶，以及编码SHE的cDNA序列。 天然纯化的SHE的特异性通常与以前已知的α-淀粉酶相似。 然而，SHE对支链淀粉的活性导致与其它α-淀粉酶明显不同的水解产物。 SHE及其在其他植物如水稻，拟南芥，苹果和马铃薯中的同源等同物可用于淀粉加工中，用于产生不同的，例如较大尺寸的工业用α限制糊精，与之前已知的 α-淀粉酶或其他淀粉水解酶。 此外，在转基因玉米植物或其他转基因生物（包括细菌，酵母和其他植物物种）中修饰该酶的表达可用于产生新的淀粉形式或改变的淀粉代谢。
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5.发明申请Identification and characterization of a novel alpha-amylase from maize endosperm 失效来自玉米胚乳的新型α-淀粉酶的鉴定和表征公开(公告)号：US20050138688A1
公开(公告)日：2005-06-23
申请号：US10952551
申请日：2004-09-27
申请人： Martha James , Alan Myers , Christophe Colleoni , Kevin Stokes
发明人： Martha James , Alan Myers , Christophe Colleoni , Kevin Stokes
CPC分类号： C12N9/2422 , C12N15/8245 , C12P19/14
摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.
摘要翻译： SHE，在玉米胚乳（Zea mays）中活性的淀粉水解酶，以及编码SHE的cDNA序列。 天然纯化的SHE的特异性通常与以前已知的α-淀粉酶相似。 然而，SHE对支链淀粉的活性导致与其它α-淀粉酶明显不同的水解产物。 SHE及其在其他植物如水稻，拟南芥，苹果和马铃薯中的同源等同物可用于淀粉加工中，用于产生不同的，例如较大尺寸的工业用α限制糊精，与之前已知的 α-淀粉酶或其他淀粉水解酶。 此外，在转基因玉米植物或其他转基因生物（包括细菌，酵母和其他植物物种）中修饰该酶的表达可用于产生新的淀粉形式或改变的淀粉代谢。
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