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1.发明授权Identification and characterization of a novel alpha-amylase from maize endosperm 失效来自玉米胚乳的新型α-淀粉酶的鉴定和表征公开(公告)号：US07270988B2
公开(公告)日：2007-09-18
申请号：US10952551
申请日：2004-09-27
CPC分类号： C12N9/2422 , C12N15/8245 , C12P19/14
摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.
摘要翻译： SHE，在玉米胚乳（Zea mays）中活性的淀粉水解酶，以及编码SHE的cDNA序列。 天然纯化的SHE的特异性通常与以前已知的α-淀粉酶相似。 然而，SHE对支链淀粉的活性导致与其它α-淀粉酶明显不同的水解产物。 SHE及其在其他植物如水稻，拟南芥，苹果和马铃薯中的同源等同物可用于淀粉加工中，用于产生不同的，例如较大尺寸的工业用α限制糊精，与之前已知的 α-淀粉酶或其他淀粉水解酶。 此外，在转基因玉米植物或其他转基因生物（包括细菌，酵母和其他植物物种）中修饰该酶的表达可用于产生新的淀粉形式或改变的淀粉代谢。
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2.发明申请Identification and characterization of a novel alpha-amylase from maize endosperm 审中-公开来自玉米胚乳的新型α-淀粉酶的鉴定和表征公开(公告)号：US20090197323A1
公开(公告)日：2009-08-06
申请号：US12317838
申请日：2008-12-30
IPC分类号： C08B30/00
CPC分类号： C12N9/2422 , C12N15/8245 , C12P19/14
摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.
摘要翻译： SHE，在玉米胚乳（Zea mays）中活性的淀粉水解酶，以及编码SHE的cDNA序列。 天然纯化的SHE的特异性通常与以前已知的α-淀粉酶相似。 然而，SHE对支链淀粉的活性导致与其它α-淀粉酶明显不同的水解产物。 SHE及其在其他植物如水稻，拟南芥，苹果和马铃薯中的同源等同物可用于淀粉加工中，用于产生不同的，例如较大尺寸的工业用α限制糊精，与之前已知的 α-淀粉酶或其他淀粉水解酶。 此外，在转基因玉米植物或其他转基因生物（包括细菌，酵母和其他植物物种）中修饰该酶的表达可用于产生新的淀粉形式或改变的淀粉代谢。
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公开(公告)号：US07495152B2
公开(公告)日：2009-02-24
申请号：US11891589
申请日：2007-08-10
CPC分类号： C12N9/2422 , C12N15/8245 , C12P19/14
摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.
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4.发明申请Identification and characterization of a novel alpha-amylase from maize endosperm 失效来自玉米胚乳的新型α-淀粉酶的鉴定和表征公开(公告)号：US20080134363A1
公开(公告)日：2008-06-05
申请号：US11891589
申请日：2007-08-10
CPC分类号： C12N9/2422 , C12N15/8245 , C12P19/14
摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.
摘要翻译： SHE，在玉米胚乳（Zea mays）中活性的淀粉水解酶，以及编码SHE的cDNA序列。 天然纯化的SHE的特异性通常与以前已知的α-淀粉酶相似。 然而，SHE对支链淀粉的活性导致与其它α-淀粉酶明显不同的水解产物。 SHE及其在其他植物如水稻，拟南芥，苹果和马铃薯中的同源等同物可用于淀粉加工中，用于产生不同的，例如较大尺寸的工业用α限制糊精，与之前已知的 α-淀粉酶或其他淀粉水解酶。 此外，在转基因玉米植物或其他转基因生物（包括细菌，酵母和其他植物物种）中修饰该酶的表达可用于产生新的淀粉形式或改变的淀粉代谢。
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公开(公告)号：US5981728A
公开(公告)日：1999-11-09
申请号：US968542
申请日：1997-11-12
申请人： Alan M. Myers , Martha G. James
发明人： Alan M. Myers , Martha G. James
IPC分类号： A01H5/00 , B01J4/02 , C07H21/04 , C12N5/10 , C12N9/00 , C12N9/10 , C12N15/09 , C12N15/29 , C12N15/82 , C12N15/84 , C12P19/04 , C12N5/04
CPC分类号： C12N9/1051 , B01J4/02 , C07H21/04 , C12N15/8245 , C12N15/8261 , C12Y204/01011 , C07K2319/00
摘要： The maize gene dull1 (du1) of the present invention is a determinant of the structure of endosperm starch. Mutations of du1 affect the activity of at least two enzymes involved in starch biosynthesis, namely the starch synthase, SSII, and the starch branching enzyme, SBEIIa. Du1 codes for a predicted 1674 residue protein, and is expressed with a unique temporal pattern in endosperm but is undetectable in leaf or root. The size of the Du1 product and its expression pattern match precisely the known characteristics of maize SSII. The Du1 product contains two different repeated regions in its unique amino terminus, one of which is identical to a conserved segment of the starch debranching enzymes. The cDNA provided for in the present invention encodes SSII, and mutations within this gene affect multiple aspects of starch biogenesis by disrupting an enzyme complex containing starch synthase(s), starch branching enzyme(s), and possibly starch debranching enzyme(s).
摘要翻译： 本发明的玉米基因dull1（du1）是胚乳淀粉结构的决定因素。 du1的突变影响淀粉生物合成中至少两种酶的活性，即淀粉合成酶，SSII和淀粉分支酶SBEIIa。 Du1编码预测的1674残基蛋白，并且在胚乳中以独特的时间模式表达，但在叶或根中是不可检测的。 Du1产品的大小及其表达模式与玉米SSII的已知特征精确匹配。 Du1产品在其独特的氨基末端含有两个不同的重复区域，其中之一与淀粉脱支酶的保守区段相同。 本发明中提供的cDNA编码SSII，并且该基因内的突变通过破坏含有淀粉合成酶，淀粉分支酶和可能的淀粉脱支酶的酶复合物影响淀粉生物发生的多个方面。
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