公开(公告)号：US20140094376A1

公开(公告)日：2014-04-03

申请号：US14035686

申请日：2013-09-24

Applicant: JIAN HAN ,  CHUNLIN WANG

Inventor： JIAN HAN ,  CHUNLIN WANG

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6869 ,  C12Q2531/113 ,  C12Q2537/143 ,  C12Q2565/301

Abstract: The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non-canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.

Abstract translation: 本公开通常涉及用于分析样品的多重方法，其包括使用多聚核苷酸扩增产生扩增产物，其中一个或多个靶序列用非干扰的，非抵消的靶特异性多核苷酸鉴定标签进行标记，通过所述扩增产物 非取消靶特异性多核苷酸鉴定标签序列，以检测一个或多个特异性多核苷酸鉴定标签的存在。 特定多核苷酸识别标签的存在与特定靶序列的存在相关。

公开(公告)号：US08561546B2

公开(公告)日：2013-10-22

申请号：US13177800

申请日：2011-07-07

Applicant: Zhenming Liu ,  Chunlin Wang ,  Shihui Duan ,  Pingwei Yin ,  Hai Yao

Inventor： Zhenming Liu ,  Chunlin Wang ,  Shihui Duan ,  Pingwei Yin ,  Hai Yao

IPC: B61F3/00 ,  B61F5/00

CPC classification number: B61F5/12

Abstract: The invention provides a bogie which comprises a bolster, two side frames and four constant contact dampers, two sides of two ends of the bolster are respectively provided with a wedge pocket; the four constant contact dampers are respectively arranged in four wedge pockets, and each of the four constant contact dampers comprises a wedge and a damping spring; the damping spring is arranged in an inner cavity of the wedge; one end of the damping spring bears against a top inner surface of the inner cavity of the wedge, and the other end of the damping spring bears against a bottom plate of the wedge pocket, wherein the bogie further comprises four through holes and four ejectors which respectively correspond to the four constant contact dampers; the through holes are arranged on the bottom plate, and the position of each of the four through holes on the bottom plate corresponds to the position where the damping spring bears against the bottom plate and a load spring of the bogie; and one end of the ejector penetrates into the damping spring, and the other end of the ejector bears against the load spring through the through hole. The bogie of the present invention solves the technical problem that the deformation of the damping springs keeps constant whether a car is empty or loaded in the prior art, and thus improves the damping effect of the bogie.

Abstract translation: 本发明提供了一种转向架，其包括摇枕，两个侧框架和四个恒定接触阻尼器，摇枕两端的两侧分别设有楔形凹槽; 四个恒定接触阻尼器分别布置在四个楔形凹穴中，四个恒定接触阻尼器中的每一个包括楔形件和阻尼弹簧; 所述阻尼弹簧设置在所述楔的内腔中; 阻尼弹簧的一端靠在楔形物的内腔的顶部内表面上，阻尼弹簧的另一端抵靠在楔形凹槽的底板上，其中转向架还包括四个通孔和四个喷射器 分别对应于四个常数接触阻尼器; 通孔布置在底板上，底板上的四个通孔中的每一个的位置对应于阻尼弹簧抵靠底板和转向架的负载弹簧的位置; 并且喷射器的一端穿过阻尼弹簧，并且喷射器的另一端通过通孔抵靠负载弹簧。 本发明的转向架解决了现有技术中汽车空载或负载时阻尼弹簧的变形保持恒定的技术问题，从而提高了转向架的阻尼效果。

公开(公告)号：US06225528B1

公开(公告)日：2001-05-01

申请号：US09143567

申请日：1998-08-28

Applicant: Chee-Kok Chin ,  Chunlin Wang ,  Jinsong Xing

Inventor： Chee-Kok Chin ,  Chunlin Wang ,  Jinsong Xing

IPC: C12N1582

CPC classification number: A01N63/02 ,  C12N15/8247 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8283

Abstract: The present invention provides pathogen-resistant transgenic plants and methods of making the plants. The transgenic plants display enhanced resistance to a variety of fungal, bacterial and viral plant pathogens due to expression of a gene that increases the unsaturated fatty acid content of the plant's cells, as compared with an equivalent, but non-transformed plant. The preferred embodiment of the invention is a plant expressing a heterologous &Dgr;-9 desaturase gene from yeast, which particularly increases cytosolic quantities of 16:1, 16:2 and 18:1 fatty acids.

Abstract translation: 本发明提供病原体抗性转基因植物和制备植物的方法。 与等效但未转化的植物相比，转基因植物由于表达增加植物细胞的不饱和脂肪酸含量的基因而显示出对各种真菌，细菌和病毒植物病原体的增强的抗性。 本发明的优选实施方案是表达来自酵母的异源DELTA-9去饱和酶基因的植物，其特别增加了16：1,16：2和18：1脂肪酸的胞质量。

公开(公告)号：US08448580B2

公开(公告)日：2013-05-28

申请号：US13165724

申请日：2011-06-21

Applicant: Zhenming Liu ,  Chunlin Wang ,  Haibin Hu ,  Shuming Xing ,  Pingwei Yin ,  Hai Yao

Inventor： Zhenming Liu ,  Chunlin Wang ,  Haibin Hu ,  Shuming Xing ,  Pingwei Yin ,  Hai Yao

IPC: B61D1/00

CPC classification number: B61F5/52 ,  B61F3/02 ,  B61F5/02

Abstract: The present invention discloses a freight car bogie and a freight car. The freight car bogie comprises two wheel sets, two side frames arranged at outer side of said wheel sets, and a bolster fixedly connected to center position of each of said two side frames, respectively. The freight car bogie further comprises a K-shaped supporting device, wherein said K-shaped supporting device passes through preset holes on two sides of an abdominal region of said bolster; a first upper end and a second upper end, as well as a first lower end and a second lower end of said K-shaped supporting device are elastically connected with said two side frames respectively; and said first upper end and second upper end, and said first lower end and second lower end are respectively symmetrical with respect to a center line of said bolster. The K-shaped supporting device of the present application may not pass through the middle part of the bolster so that a reinforcing rib for bearing vertical load can be disposed on the middle part of the bolster, thereby increasing the anti-lozenge-distortion rigidity of the bogie as well as improving the strength of the bolster for bearing the vertical load.

Abstract translation: 本发明公开了一种货车转向架和货车。 货车转向架包括两个轮组，布置在所述轮组的外侧的两个侧框架和分别固定地连接到所述两个侧框架中的每一个的中心位置的撑杆。 货车转向架还包括K形支撑装置，其中所述K形支撑装置通过所述枕垫的腹部区域的两侧上的预定孔; 所述K形支撑装置的第一上端和第二上端以及第一下端和第二下端分别与所述两个侧框架弹性连接; 并且所述第一上端和第二上端，并且所述第一下端和第二下端分别相对于所述垫的中心线对称。 本申请的K形支撑装置可能不能通过垫片的中间部分，从而可以在支撑件的中间部分设置用于承载垂直载荷的加强肋，从而增加抗弯曲变形刚度 转向架以及提高承重垂直载荷的承重力。

公开(公告)号：US09938578B2

公开(公告)日：2018-04-10

申请号：US14035686

申请日：2013-09-24

Applicant: Jian Han ,  Chunlin Wang

Inventor： Jian Han ,  Chunlin Wang

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6869 ,  C12Q2531/113 ,  C12Q2537/143 ,  C12Q2565/301

Abstract: The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non-canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.

公开(公告)号：US20130008337A1

公开(公告)日：2013-01-10

申请号：US13177800

申请日：2011-07-07

Applicant: Zhenming Liu ,  Chunlin Wang ,  Shihui Duan ,  Pingwei Yin ,  Hai Yao

Inventor： Zhenming Liu ,  Chunlin Wang ,  Shihui Duan ,  Pingwei Yin ,  Hai Yao

IPC: B61F5/50

CPC classification number: B61F5/12

Abstract: The invention provides a bogie which comprises a bolster, two side frames and four constant contact dampers, two sides of two ends of the bolster are respectively provided with a wedge pocket; the four constant contact dampers are respectively arranged in four wedge pockets, and each of the four constant contact dampers comprises a wedge and a damping spring; the damping spring is arranged in an inner cavity of the wedge; one end of the damping spring bears against a top inner surface of the inner cavity of the wedge, and the other end of the damping spring bears against a bottom plate of the wedge pocket, wherein the bogie further comprises four through holes and four ejectors which respectively correspond to the four constant contact dampers; the through holes are arranged on the bottom plate, and the position of each of the four through holes on the bottom plate corresponds to the position where the damping spring bears against the bottom plate and a load spring of the bogie; and one end of the ejector penetrates into the damping spring, and the other end of the ejector bears against the load spring through the through hole. The bogie of the present invention solves the technical problem that the deformation of the damping springs keeps constant whether a car is empty or loaded in the prior art, and thus improves the damping effect of the bogie.

Abstract translation: 本发明提供了一种转向架，其包括摇枕，两个侧框架和四个恒定接触阻尼器，摇枕两端的两侧分别设有楔形凹槽; 四个恒定接触阻尼器分别布置在四个楔形凹穴中，四个恒定接触阻尼器中的每一个包括楔形件和阻尼弹簧; 所述阻尼弹簧设置在所述楔的内腔中; 阻尼弹簧的一端靠在楔形物的内腔的顶部内表面上，阻尼弹簧的另一端抵靠在楔形凹槽的底板上，其中转向架还包括四个通孔和四个喷射器 分别对应于四个常数接触阻尼器; 通孔布置在底板上，底板上的四个通孔中的每一个的位置对应于阻尼弹簧抵靠底板和转向架的负载弹簧的位置; 并且喷射器的一端穿过阻尼弹簧，并且喷射器的另一端通过通孔抵靠负载弹簧。 本发明的转向架解决了现有技术中汽车空载或负载时阻尼弹簧的变形保持恒定的技术问题，从而提高了转向架的阻尼效果。

公开(公告)号：US20110247520A1

公开(公告)日：2011-10-13

申请号：US13165724

申请日：2011-06-21

Applicant: ZHENMING LIU ,  Chunlin Wang ,  Haibin Hu ,  Shuming Xing ,  Pingwei Yin ,  Hai Yao

Inventor： ZHENMING LIU ,  Chunlin Wang ,  Haibin Hu ,  Shuming Xing ,  Pingwei Yin ,  Hai Yao

IPC: B61F5/00 ,  B61F5/04 ,  B61F3/00

CPC classification number: B61F5/52 ,  B61F3/02 ,  B61F5/02

Abstract: The present invention discloses a freight car bogie and a freight car. The freight car bogie comprises two wheel sets, two side frames arranged at outer side of said wheel sets, and a bolster fixedly connected to center position of each of said two side frames, respectively. The freight car bogie further comprises a K-shaped supporting device, wherein said K-shaped supporting device passes through preset holes on two sides of an abdominal region of said bolster; a first upper end and a second upper end, as well as a first lower end and a second lower end of said K-shaped supporting device are elastically connected with said two side frames respectively; and said first upper end and second upper end, and said first lower end and second lower end are respectively symmetrical with respect to a centre line of said bolster. The K-shaped supporting device of the present application may not pass through the middle part of the bolster so that a reinforcing rib for bearing vertical load can be disposed on the middle part of the bolster, thereby increasing the anti-lozenge-distortion rigidity of the bogie as well as improving the strength of the bolster for bearing the vertical load.

Abstract translation: 本发明公开了一种货车转向架和货车。 货车转向架包括两个轮组，布置在所述轮组的外侧的两个侧框架和分别固定地连接到所述两个侧框架中的每一个的中心位置的撑杆。 货车转向架还包括K形支撑装置，其中所述K形支撑装置通过所述枕垫的腹部区域的两侧上的预定孔; 所述K形支撑装置的第一上端和第二上端以及第一下端和第二下端分别与所述两个侧框架弹性连接; 并且所述第一上端和第二上端，并且所述第一下端和第二下端分别相对于所述垫的中心线对称。 本申请的K形支撑装置可能不能通过垫片的中间部分，从而可以在支撑件的中间部分设置用于承载垂直载荷的加强肋，从而增加抗弯曲变形刚度 转向架以及提高承重垂直载荷的承重力。