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公开(公告)号:US07887028B2
公开(公告)日:2011-02-15
申请号:US10489324
申请日:2002-09-18
申请人: Paul Clifford Reid
发明人: Paul Clifford Reid
IPC分类号: A01K3/00
CPC分类号: A01K3/005
摘要: An electric fence to span a given region between two substantially opposing supports, the fence including one or more insulators coupled to each opposing support; an electrical power source electrically connected to a first conductive strand and configured to apply an electrical current thereto, at least one further electrically distinct conductive strand coupled to the insulators and at a different electric potential than the first strand, characterised in that at least one insulator includes a substantially non-conductive element having one or more confined pathways, the element rotatable about a central axis orientated to maintain symmetrical revolution and wherein the conductive strands extend continuously and repeatedly between each support, each conductive strand in contact with at least one of the insulators.
摘要翻译: 电栅栏,跨越两个基本上相对的支撑件之间的给定区域,所述围栏包括联接到每个相对支撑件的一个或多个绝缘体; 电连接到第一导电线并且被配置为向其施加电流的电源,至少一个与绝缘体耦合并且处于与第一线不同的电位的另外不同的导电线,其特征在于,至少一个绝缘体 包括具有一个或多个限制通路的基本上不导电的元件,所述元件可围绕中心轴线旋转,所述中心轴线定向成保持对称的旋转,并且其中所述导电股线在每个支撑件之间连续且重复地延伸,每个导电股线与至少一个 绝缘子
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公开(公告)号:US20090318304A1
公开(公告)日:2009-12-24
申请号:US12325922
申请日:2008-12-01
申请人: Radoje Drmanac , Clifford Reid
发明人: Radoje Drmanac , Clifford Reid
IPC分类号: C40B30/04
CPC分类号: C12Q1/6869 , C12Q1/6874 , C12Q2549/119 , C12Q2537/149 , C12Q2565/137
摘要: Methods are provided for efficient shotgun sequencing to allow efficient selection and sequencing of nucleic acids of interest contained in a library. The nucleic acids of interest can be defined any time before or after preparation of the library. One example of nucleic acids of interest is missing or low confidence genome sequences resulting from an initial sequencing procedure. Other nucleic acids of interest include subsets of genomic DNA, RNA or cDNAs (exons, genes, gene sets, transciptomes). By designing an efficient (simple to implement, speedy, high specificity, low cost) selection procedure, a more complete sequence is achieved with less effort than by using highly redundant shotgun sequencing in an initial sequencing procedure
摘要翻译: 提供了有效的霰弹枪测序的方法,以允许文库中包含的感兴趣的核酸的有效选择和测序。 感兴趣的核酸可以在文库制备之前或之后的任何时间进行定义。 感兴趣的核酸的一个实例是由初始测序程序产生的缺失或低置信基因组序列。 感兴趣的其他核酸包括基因组DNA,RNA或cDNAs(外显子,基因,基因组,转录本)的子集。 通过设计一种高效(简单实施,快速,高特异性,低成本)选择程序,通过在初始测序程序中使用高度冗余的霰弹枪测序,以更少的努力实现更完整的顺序
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公开(公告)号:US20080308299A1
公开(公告)日:2008-12-18
申请号:US11914243
申请日:2006-05-12
IPC分类号: H01B17/00
摘要: A connection apparatus (1) is provided. The apparatus has: a body (7); at least two connectors (6a, 6b); and an interconnection element (16). The wire connectors (6a, 6b) connect wires to the apparatus (1) and provide a conductive connection of the wires to an interconnection element (16).
摘要翻译: 提供一种连接装置(1)。 该装置具有:主体(7); 至少两个连接器(6a,6b); 和互连元件(16)。 电线连接器(6a,6b)将电线连接到设备(1),并且将导线连接到互连元件(16)。
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公开(公告)号:US20090176234A1
公开(公告)日:2009-07-09
申请号:US12335188
申请日:2008-12-15
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6874 , C12Q1/6855 , C12Q2531/125 , C12Q2521/313 , C12Q2521/125 , C12Q2537/163 , C12Q2525/191 , C12Q2521/531 , C12Q2521/501 , C12Q2525/307 , C12Q2525/151
摘要: The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译: 本发明涉及用于核酸鉴定和检测的组合物和方法。 本发明的组合物和方法包括从样品中提取和分离靶核酸,使用片段化的靶核酸产生靶核酸模板,并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。 本发明还包括使用各种测序应用检测和鉴定序列的方法,包括通过连接方法测序。
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公开(公告)号:US06962327B2
公开(公告)日:2005-11-08
申请号:US10044721
申请日:2002-01-14
申请人: Paul Clifford Reid
发明人: Paul Clifford Reid
摘要: A fence device and method for constructing a fence support which comprises a sleeve for over a supporting member such as a rod extending vertically from a concrete base. The sleeve can be constructed of extruded insulating material and includes deformable internal projections to secure the sleeve by friction to the supporting member. The sleeve comprises at least one web or flange extending longitudinally and normally therefrom which has slots or incisions therein for a supporting lengths of material such as electrified wires. In one embodiment a pair of slots are disposed at opposing angles to facilitate securing lengths of wire.
摘要翻译: 一种用于构造围栏支撑件的围栏装置和方法,其包括用于在诸如从混凝土基座垂直延伸的杆的支撑构件上方的套筒。 套筒可以由挤压绝缘材料构成,并且包括可变形的内部突起,以通过摩擦将支撑件固定到支撑构件上。 该套筒包括至少一个从其纵向和正常延伸的腹板或凸缘,其中具有槽或切口用于支撑长度的材料,例如带电的导线。 在一个实施例中,一对狭槽以相对的角度设置,以有助于确保线的长度。
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公开(公告)号:US20100105052A1
公开(公告)日:2010-04-29
申请号:US12573697
申请日:2009-10-05
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6874 , C12Q2533/107 , C12Q2525/185 , C12Q2525/151
摘要: The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译: 本发明涉及用于核酸鉴定和检测的组合物和方法。 本发明的组合物和方法包括从样品中提取和分离靶核酸,使用片段化的靶核酸产生靶核酸模板,并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。 本发明还包括使用各种测序应用检测和鉴定序列的方法,包括通过连接方法测序。
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公开(公告)号:US20120100534A1
公开(公告)日:2012-04-26
申请号:US12329365
申请日:2008-12-05
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6874 , C12Q1/6855 , C12Q2531/125 , C12Q2521/313 , C12Q2521/125 , C12Q2537/163 , C12Q2525/191 , C12Q2521/531 , C12Q2521/501 , C12Q2525/307 , C12Q2525/151
摘要: The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译: 本发明涉及用于核酸鉴定和检测的组合物和方法。 本发明的组合物和方法包括从样品中提取和分离靶核酸,使用片段化的靶核酸产生靶核酸模板,并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。 本发明还包括使用各种测序应用检测和鉴定序列的方法,包括通过连接方法测序。
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公开(公告)号:US08298768B2
公开(公告)日:2012-10-30
申请号:US12325922
申请日:2008-12-01
申请人: Radoje Drmanac , Clifford Reid
发明人: Radoje Drmanac , Clifford Reid
CPC分类号: C12Q1/6869 , C12Q1/6874 , C12Q2549/119 , C12Q2537/149 , C12Q2565/137
摘要: Methods are provided for efficient shotgun sequencing to allow efficient selection and sequencing of nucleic acids of interest contained in a library. The nucleic acids of interest can be defined any time before or after preparation of the library. One example of nucleic acids of interest is missing or low confidence genome sequences resulting from an initial sequencing procedure. Other nucleic acids of interest include subsets of genomic DNA, RNA or cDNAs (exons, genes, gene sets, transciptomes). By designing an efficient (simple to implement, speedy, high specificity, low cost) selection procedure, a more complete sequence is achieved with less effort than by using highly redundant shotgun sequencing in an initial sequencing procedure.
摘要翻译: 提供了有效的霰弹枪测序的方法,以允许文库中包含的感兴趣的核酸的有效选择和测序。 感兴趣的核酸可以在文库制备之前或之后的任何时间进行定义。 感兴趣的核酸的一个实例是由初始测序程序产生的缺失或低置信基因组序列。 感兴趣的其他核酸包括基因组DNA,RNA或cDNAs(外显子,基因,基因组,转录本)的子集。 通过设计一种高效(简单易于实施,快速,高特异性,低成本)选择程序,通过在初始测序程序中使用高度冗余的霰弹枪测序,以更少的努力实现更完整的顺序。
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公开(公告)号:US20110193040A1
公开(公告)日:2011-08-11
申请号:US13054920
申请日:2009-07-06
申请人: Paul Clifford Reid
发明人: Paul Clifford Reid
CPC分类号: E04H17/266 , A01K3/005 , E04H17/06
摘要: A tensioner for a fencing wire reel, which includes a tensioning member, and a housing for the tensioning member, wherein the housing and the tensioning member are configured such that when fencing wire passes through the housing in one direction, the tensioning member applies a first biasing force against the wire, and when fencing wire passes through the housing in the opposite direction, the tensioning member applies a second biasing force against the wire, the second biasing force being less than the first biasing force. A portable fence system for constructing a fence, which includes at least one fencing wire reel, and a reel housing for the at least one fencing wire reel, and includes the tensioner as described is also claimed.
摘要翻译: 一种用于围栏线卷轴的张紧器,其包括张紧构件和用于张紧构件的壳体,其中,所述壳体和所述张紧构件被构造成使得当围栏线沿一个方向穿过所述壳体时,所述张紧构件施加第一 当线圈沿相反方向穿过壳体时,张紧构件对线施加第二偏压力,第二偏压力小于第一偏压力。 一种用于构造围栏的便携式围栏系统,其包括至少一个围栏线卷轴和用于至少一个击剑线卷轴的卷轴壳体,并且还包括如上所述的张紧器。
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