公开(公告)号：US4666831A

公开(公告)日：1987-05-19

申请号：US702555

申请日：1985-02-19

申请人： Andrew S. Janoff ,  Joyce Rauch ,  Colin P. S. Tilcock

发明人： Andrew S. Janoff ,  Joyce Rauch ,  Colin P. S. Tilcock

IPC分类号： G01N33/86 ,  G01N33/92 ,  G01N33/53 ,  G01N33/571

CPC分类号： G01N33/92 ,  G01N33/86 ,  G01N2405/04 ,  Y10S436/811 ,  Y10S436/825 ,  Y10T436/25125

摘要： Improved lipid-dependent diagnostic assays are provided wherein the test sample to be assayed is pre-incubated with one or more phospholipids having a hexagonal (H.sub.II) organization or with lipidic particles. The pre-incubation results in reduced false positives due to anti-phospholipid antibodies, such as, lupus anticoagulants, which may be present in the test sample, without changing the overall character, including the normal baseline, of the assay. Suprisingly, in accordance with the invention, it has been found that only hexagonal phospholipids and lipidic particles can be used, and, in particular, lamellar (bilayer) phospholipids cannot be used. An assay for diagnosing systemic lupus erythematosus (SLE) is also provided.

摘要翻译： 提供改进的脂质依赖性诊断测定法，其中待测试的测试样品与一种或多种具有六方（HII）组织或脂质颗粒的磷脂预孵育。 预孵育导致由于抗磷脂抗体（例如可能存在于测试样品中的狼疮抗凝剂）而导致的假阳性减少，而不改变测定的总体特征，包括正常基线。 令人惊奇的是，根据本发明，已经发现只能使用六方磷脂和脂质颗粒，特别是不能使用层状（双层）磷脂。 还提供了用于诊断系统性红斑狼疮（SLE）的测定法。

公开(公告)号：US4923854A

公开(公告)日：1990-05-08

申请号：US821366

申请日：1986-01-22

申请人： Colin P. S. Tilcock ,  Pieter R. Cullis ,  Michael J. Hope ,  Andrew S. Janoff

发明人： Colin P. S. Tilcock ,  Pieter R. Cullis ,  Michael J. Hope ,  Andrew S. Janoff

IPC分类号： A61K9/00 ,  A61K9/08 ,  A61K9/10 ,  A61K9/127 ,  A61K31/57 ,  A61K31/685 ,  A61K38/00 ,  A61K38/22 ,  A61K45/00 ,  A61K47/00 ,  A61K47/24

CPC分类号： A61K9/1277 ,  A61K47/24 ,  A61K9/0019

摘要： A method and composition are described for the solubilization of hydrophobic materials using a lysophospholipid. The method includes drying a composition comprising a hydrophobic material-solubilizing effective amount of phospholipid from organic solvent and hydrating the resulting film with an aqueous medium at either a pH of between about 8.5 and about 14.0, or at pH 7.0 followed by reduction of the temperature to less then 0.degree. C.

摘要翻译： 描述了使用溶血磷脂增溶疏水性材料的方法和组合物。 该方法包括从有机溶剂中干燥含有疏水物质增溶有效量的磷脂的组合物，并用pH介于约8.5至约14.0之间或pH 7.0的水性介质水合所得膜，随后降低温度 小于0℃

公开(公告)号：US4698299A

公开(公告)日：1987-10-06

申请号：US831255

申请日：1986-02-19

申请人： Andrew S. Janoff ,  Joyce Rauch ,  Colin P. S. Tilcock

发明人： Andrew S. Janoff ,  Joyce Rauch ,  Colin P. S. Tilcock

IPC分类号： G01N33/86 ,  G01N33/92 ,  G01N33/564 ,  G01N33/571 ,  G01N33/96

CPC分类号： G01N33/86 ,  G01N33/92 ,  G01N2405/04 ,  Y10S435/962 ,  Y10S436/825 ,  Y10T436/25125

摘要： Lipid-dependent diagnostic assays are provided wherein the test sample to be assayed is pre-incubated with one or more phospholipids having a hexagonal (H.sub.II) organization, with lipidic particles, or with bilayer-forming lysophospholipids such as monooleoylphosphatidylethanolamine (MOPE). The pre-incubation results in reduced false positives due to antiphospholipid antibodies, such as, lupus anticoagulants, which may be present in the test sample, without changing the overall character, including the normal baseline, of the assay. Surprisingly, in accordance with the invention, it has been found that only hexagonal phospholipids, lipidic particles, and bilayer-forming lysophospholipids can be used, and, in particular, lamellar (bilayer) phospholipids (other than such lysophospholipids) cannot be used. An assay for diagnosing systemic lupus erythematosus (SLE) is also provided.

摘要翻译： 提供了脂质依赖性诊断测定法，其中将要测定的测试样品与一种或多种具有六方晶（HII）组织的磷脂，脂质颗粒或双层形成溶血磷脂如单油酰磷脂酰乙醇胺（MOPE）预温育。 预孵育导致由于抗磷脂抗体（例如可能存在于测试样品中的狼疮抗凝剂）而导致的假阳性减少，而不改变测定的总体特征，包括正常基线。 令人惊奇的是，根据本发明，已经发现只能使用六方磷脂，脂质颗粒和双层形成溶血磷脂，特别是不能使用层状（双层）磷脂（除这种溶血磷脂之外）。 还提供了用于诊断系统性红斑狼疮（SLE）的测定法。