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公开(公告)号:US20070028322A1
公开(公告)日:2007-02-01
申请号:US11542046
申请日:2006-10-03
申请人: Mark Dizigan , Rebecca Kelly , Dale Voyles , Michael Luethy , Thomas Malvar , Kathleen Malloy
发明人: Mark Dizigan , Rebecca Kelly , Dale Voyles , Michael Luethy , Thomas Malvar , Kathleen Malloy
CPC分类号: C12N15/8254 , A01H5/10 , C12Q1/6895
摘要: Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.
摘要翻译: 本文公开了基于插入到玉米基因组中的外源DNA构建体的DNA序列和插入位点侧翼的基因组序列来检测赖氨酸增加的转基因事件的存在的测定。 还提供了具有表达二氢吡啶甲酸合酶的新型外源DNA构建体的转基因植物,其活性导致植物或植物产物中赖氨酸增加。
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公开(公告)号:US20050250938A1
公开(公告)日:2005-11-10
申请号:US10660097
申请日:2003-09-11
申请人: Alan Kriz , Michael Luethy , Dale Voyles
发明人: Alan Kriz , Michael Luethy , Dale Voyles
CPC分类号: C12N15/8222 , C07K14/415 , C12N15/8216
摘要: Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus Coix for transformation. Included in these transgene sequences are Coix promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.
摘要翻译: 公开了在单子叶植物(包括玉米)中表达转基因的方法和组合物。 在本发明中,通过使用来自Coix属植物的单子叶 - 同源序列来避免转基因沉默。 这些转基因序列中包括Coix启动子,增强子,编码序列和终止子。 基于同源性的基因沉默可以限制或有效地消除转基因表达,玉米衍生的转基因的合适替代物对于在玉米中表达是期望的。
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公开(公告)号:US20050132437A1
公开(公告)日:2005-06-16
申请号:US11004221
申请日:2004-12-02
申请人: Mark Dizigan , Rebecca Kelly , Dale Voyles , Michael Luethy , Thomas Malvar , Kathleen Malloy
发明人: Mark Dizigan , Rebecca Kelly , Dale Voyles , Michael Luethy , Thomas Malvar , Kathleen Malloy
CPC分类号: C12N15/8254 , A01H5/10 , C12Q1/6895
摘要: Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.
摘要翻译: 本文公开了基于插入到玉米基因组中的外源DNA构建体的DNA序列和插入位点侧翼的基因组序列来检测赖氨酸增加的转基因事件的存在的测定。 还提供了具有表达二氢吡啶甲酸合酶的新型外源DNA构建体的转基因植物,其活性导致植物或植物产物中赖氨酸增加。
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