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公开(公告)号：US5665576A
公开(公告)日：1997-09-09
申请号：US476286
申请日：1995-06-07
IPC分类号： A01H4/00 , C07D305/14 , C07D493/00 , C12N5/04 , C12P13/02 , C12P15/00 , C12P17/02 , C12P19/60 , C12R1/91 , C12P1/00 , C12N5/00 , C12P7/22
摘要： A method for the production of taxanes such as taxol. The method includes inducing formation of callus cells by contacting an explant tissue with a liquid medium without complete submergence of the tissue in the medium. The callus cells formed are employed in a liquid suspension cell culture to produce one or more taxanes.
摘要翻译： 一种生产紫杉烷类紫杉醇的方法。 该方法包括通过使外植体组织与液体培养基接触而诱导愈伤组织细胞的形成，而不会在培养基中完全淹没组织。 所形成的愈伤组织细胞用于液体悬浮细胞培养物中以产生一种或多种紫杉烷类。
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2.发明授权Callus cell induction from partially submerged explant tissue in liquid medium for preparation of taxanes 失效从液体培养基中部分浸没的外植体组织诱导愈伤组织，用于制备紫杉烷
公开(公告)号：US5527702A
公开(公告)日：1996-06-18
申请号：US202718
申请日：1994-02-23
IPC分类号： A01H4/00 , C07D305/14 , C07D493/00 , C12N5/04 , C12P13/02 , C12P15/00 , C12P17/02 , C12P19/60 , C12R1/91 , C12N5/00 , C12P17/04
摘要： A method for the induction of callus cells capable of producing taxanes from explant tissue by partially submerging the explant tissue in a liquid culture medium on a membrane raft, resulting in callus formation. The induction of callus cells by this method provides direct transfer of the cells formed to a liquid medium for a suspension cell culture preparation of taxanes. Thus, there is no need of a separate growth or proliferation step in the method of this invention. Furthermore, callus cells can be produced by the method; and also disclosed is a method for the use of the callus cells so produced in a suspension cell culture preparation for taxanes.
摘要翻译： 一种诱导能够从外植体组织制备紫杉烷的愈伤组织细胞的方法，其通过将液体培养基中的外植体组织部分浸没在膜筏上，导致愈伤组织形成。 通过该方法诱导愈伤组织细胞提供形成的细胞直接转移到用于紫杉烷类的悬浮细胞培养制剂的液体培养基中。 因此，在本发明的方法中不需要单独的生长或增殖步骤。 此外，可以通过该方法产生愈伤组织细胞; 并且还公开了在紫杉烷的悬浮细胞培养制备中使用如此产生的愈伤组织细胞的方法。
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