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公开(公告)号：US5534427A
公开(公告)日：1996-07-09
申请号：US923120
申请日：1992-07-31
CPC分类号： C12N9/20
摘要： Nucleic acid sequences, particularly DNA sequences, coding for all or part of a lipase of Pseudomonas fluorescens, wherein said lipase has the amino acid sequence encoded by the nucleotide sequence of the BamHI/HindIII insert of the plasmid pRJ-Ltac1 from Escherichia coli BL21/pRJ-Ltac1 deposited with the American Type Culture Collection, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods utilizing these materials. The invention also concerns polypeptide molecules comprising all or part of a lipase of Pseudomonas fluorescens, wherein said lipase has the amino acid sequence encoded by the nucleotide sequence of the plasmid pRJ-Ltac1 from Escherichia coli BL21/pRJ-Ltac1 BamHI/HindIII insert of deposited with the American Type Culture Collection, and methods for producing these polypeptides.
摘要翻译： 核酸序列，特别是DNA序列，其编码荧光假单胞菌的全部或部分脂肪酶，其中所述脂肪酶具有由来自大肠杆菌BL21 / P21的质粒pRJ-Ltac1的BamHI / HindIII插入片段的核苷酸序列编码的氨基酸序列， 保藏在美国典型培养物保藏中心的pRJ-Ltac1，含有DNA序列的表达载体，含有表达载体的宿主细胞，以及利用这些材料的方法。 本发明还涉及包含荧光假单胞菌的全部或部分脂肪酶的多肽分子，其中所述脂肪酶具有由大肠杆菌BL21 / pRJ-Ltac1 BamHI / HindIII插入片段的沉积的质粒pRJ-Ltac1的核苷酸序列编码的氨基酸序列 美国典型培养物保藏中心，以及生产这些多肽的方法。
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公开(公告)号：US07087418B2
公开(公告)日：2006-08-08
申请号：US10320300
申请日：2002-12-16
CPC分类号： C12N9/0004 , C12Q1/26 , G01N2333/04
摘要： This invention relates to a recombinant Pichia pastoris formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding P. pastoris FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant P. pastoris FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to P. pastoris FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated P. pastoris FDH nucleic acids, polypeptides, and antibodies, and methods of using P. pastoris FDH in various reactions for industrial or pharmaceutical applications.
摘要翻译： 本发明涉及催化甲酸氧化成二氧化碳并同时将烟酰胺腺嘌呤二核苷酸（NAD +）还原为还原形式（NADH）的重组巴斯德毕赤酵母甲酸脱氢酶（FDH）酶。 还涉及编码巴斯德毕赤酵母多肽的分离的核酸及其片段和变体，以及包含这些核酸的载体和宿主细胞。 进一步相关的是分离的重组巴斯德毕赤酵母FDH多肽及其片段和变体，以及特异性结合巴斯德毕赤酵母多肽，片段或变体的抗体。 本发明还涉及获得分离的巴斯德毕赤酵母FDH核酸，多肽和抗体的方法，以及在工业或药物应用的各种反应中使用巴斯德毕赤酵母FDH的方法。
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公开(公告)号：US06929935B2
公开(公告)日：2005-08-16
申请号：US10320104
申请日：2002-12-16
CPC分类号： C12N9/0004 , C12Q1/26 , C12Q1/32 , G01N2333/04
摘要： This invention relates to an novel Gluconobacter oxydans 2-ketoreductase useful for the synthesis of chiral alcohols. Also related are isolated nucleic acids encoding G. oxydans 2-ketoreductase enzymes, and enzyme fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated G. oxydans 2-ketoreductase polypeptides, and fragments and variants thereof, and antibodies that specifically bind to G. oxydans 2-ketoreductase polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated G. oxydans 2-ketoreductase nucleic acids, polypeptides, and antibodies, and methods of using G. oxydans 2-ketoreductase in various reactions for industrial or pharmaceutical applications.
摘要翻译： 本发明涉及可用于合成手性醇的新型氧化葡糖杆菌2-酮还原酶。 还涉及编码G.oxydans 2-酮还原酶的分离的核酸及其酶片段及其变体，以及包含这些核酸的载体和宿主细胞。 进一步相关的是分离的G.oxydans 2-酮还原酶多肽及其片段和变体，以及特异性结合G.oxydans 2-酮还原酶多肽，片段或变体的抗体。 本发明还涉及获得分离的G.oxydans 2-酮还原酶核酸，多肽和抗体的方法，以及在工业或药物应用的各种反应中使用G.oxydans 2-酮还原酶的方法。
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4.发明授权Enzymatic hydroxylation process for the preparation of HMG-CoA reductase inhibitors and intermediates thereof 失效用于制备HMG-CoA还原酶抑制剂的酶促羟基化方法及其中间体
公开(公告)号：US6043064A
公开(公告)日：2000-03-28
申请号：US141316
申请日：1993-10-22
申请人： Brian L. Davis , Paul M. Cino , Laszlo Szarka
发明人： Brian L. Davis , Paul M. Cino , Laszlo Szarka
IPC分类号： C12P7/40 , A61K31/215 , A61P3/06 , C12P7/02 , C12P7/62 , C12P17/06 , C12R1/01 , C12R1/465 , C12R1/645 , C12P7/00
CPC分类号： C12P7/62 , C12P17/06 , C12P7/02 , Y10S435/872 , Y10S435/886
摘要： An enzymatic hydroxylation process for the preparation of compounds useful as HMG-CoA reductase inhibitors and/or as intermediates in the preparation of HMG-CoA reductase inhibitors uses a microorganism or an enzyme derived from, or having the structure of an enzyme derived from, said microorganism, which is capable of catalyzing the hydroxylation process.
摘要翻译： 用于制备用作HMG-CoA还原酶抑制剂和/或作为制备HMG-CoA还原酶抑制剂的中间体的化合物的酶促羟化方法使用来源于或具有来源于所述 微生物，其能够催化羟基化过程。
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公开(公告)号：US5665576A
公开(公告)日：1997-09-09
申请号：US476286
申请日：1995-06-07
IPC分类号： A01H4/00 , C07D305/14 , C07D493/00 , C12N5/04 , C12P13/02 , C12P15/00 , C12P17/02 , C12P19/60 , C12R1/91 , C12P1/00 , C12N5/00 , C12P7/22
摘要： A method for the production of taxanes such as taxol. The method includes inducing formation of callus cells by contacting an explant tissue with a liquid medium without complete submergence of the tissue in the medium. The callus cells formed are employed in a liquid suspension cell culture to produce one or more taxanes.
摘要翻译： 一种生产紫杉烷类紫杉醇的方法。 该方法包括通过使外植体组织与液体培养基接触而诱导愈伤组织细胞的形成，而不会在培养基中完全淹没组织。 所形成的愈伤组织细胞用于液体悬浮细胞培养物中以产生一种或多种紫杉烷类。
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6.发明授权Enzymatic hydrolysis and esterification processes for the preparation of HMG-CoA reductase inhibitors and intermediates thereof 失效用于制备HMG-CoA还原酶抑制剂的酶水解和酯化方法及其中间体
公开(公告)号：US5620876A
公开(公告)日：1997-04-15
申请号：US875859
申请日：1992-04-29
申请人： Brian L. Davis , Paul M. Cino , Laszlo J. Szarka
发明人： Brian L. Davis , Paul M. Cino , Laszlo J. Szarka
IPC分类号： C12P7/42 , C12P17/06 , C12P7/40 , A61K31/425
摘要： Enzymatic hydrolysis and esterification processes for the preparation of compounds useful as HMG-CoA reductase inhibitors and/or as intermediates in the preparation of HMG-CoA reductase inhibitors.
摘要翻译： 用于制备用作HMG-CoA还原酶抑制剂的化合物和/或作为制备HMG-CoA还原酶抑制剂的中间体的酶水解和酯化方法。
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7.发明授权Callus cell induction from partially submerged explant tissue in liquid medium for preparation of taxanes 失效从液体培养基中部分浸没的外植体组织诱导愈伤组织，用于制备紫杉烷
公开(公告)号：US5527702A
公开(公告)日：1996-06-18
申请号：US202718
申请日：1994-02-23
IPC分类号： A01H4/00 , C07D305/14 , C07D493/00 , C12N5/04 , C12P13/02 , C12P15/00 , C12P17/02 , C12P19/60 , C12R1/91 , C12N5/00 , C12P17/04
摘要： A method for the induction of callus cells capable of producing taxanes from explant tissue by partially submerging the explant tissue in a liquid culture medium on a membrane raft, resulting in callus formation. The induction of callus cells by this method provides direct transfer of the cells formed to a liquid medium for a suspension cell culture preparation of taxanes. Thus, there is no need of a separate growth or proliferation step in the method of this invention. Furthermore, callus cells can be produced by the method; and also disclosed is a method for the use of the callus cells so produced in a suspension cell culture preparation for taxanes.
摘要翻译： 一种诱导能够从外植体组织制备紫杉烷的愈伤组织细胞的方法，其通过将液体培养基中的外植体组织部分浸没在膜筏上，导致愈伤组织形成。 通过该方法诱导愈伤组织细胞提供形成的细胞直接转移到用于紫杉烷类的悬浮细胞培养制剂的液体培养基中。 因此，在本发明的方法中不需要单独的生长或增殖步骤。 此外，可以通过该方法产生愈伤组织细胞; 并且还公开了在紫杉烷的悬浮细胞培养制备中使用如此产生的愈伤组织细胞的方法。
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