公开(公告)号：US06420121B1

公开(公告)日：2002-07-16

申请号：US09387044

申请日：1999-08-31

申请人： Jay Nelson ,  Daniel Streblow ,  Cecilia Soderberg-Naucler ,  Patricia Smith ,  Fronziska Ruchti

发明人： Jay Nelson ,  Daniel Streblow ,  Cecilia Soderberg-Naucler ,  Patricia Smith ,  Fronziska Ruchti

IPC分类号： G01N3353

CPC分类号： G01N33/5061 ,  A61K31/00 ,  A61K31/395 ,  G01N33/5008 ,  G01N33/5029 ,  G01N33/566

摘要： There is disclosed an assay system for determining therapeutic activity for treating restenosis, atherosclerosis, chronic rejection syndrome and graft versus host disease (GVHD) by measuring inhibition of cell migration activity in smooth muscle cells expressing a US28 receptor from the CMV genome. Specifically, there is disclosed a method for measuring inhibition of cell migration in isolated cells transfected with US28 or infected with CMV and stimulated with a ligand. There is further disclosed a method for treating atherosclerosis, restenosis, chronic rejection syndrome and graft versus host disease (GVHD), comprising administering an effective amount of an agent that is a US28 receptor antagonist, wherein a US28 receptor antagonist comprises an inhibitor compound that prevents transduction of US28 receptor signal stimulated by a US28 receptor ligand, wherein a US28 receptor ligand is selected from the group consisting of RANTES, MIP-1&agr; and MCP. The invention further provides a method for treating restenosis, atherosclerosis, chronic rejection syndrome and GVHD by administering KHSV encoded vMIP-2, fractalkine or herbimycin.

摘要翻译： 公开了通过测量来自CMV基因组的表达US28受体的平滑肌细胞中细胞迁移活性的抑制来确定治疗再狭窄，动脉粥样硬化，慢性排斥综合征和移植物抗宿主病（GVHD）的治疗活性的测定系统。 具体地，公开了一种测量用US28转染或用CMV感染并用配体刺激的分离的细胞中细胞迁移抑制的方法。 还公开了一种治疗动脉粥样硬化，再狭窄，慢性排斥综合征和移植物抗宿主病（GVHD）的方法，包括给予有效量的US28受体拮抗剂的试剂，其中US28受体拮抗剂包含防止 US28受体信号被US28受体配体刺激的转导，其中US28受体配体选自RANTES，MIP-1α和MCP。 本发明还提供了一种通过施用编码的VHS-2，fractalkine或者草甘膦来治疗再狭窄，动脉粥样硬化，慢性排斥综合征和GVHD的方法。

公开(公告)号：US20170146519A1

公开(公告)日：2017-05-25

申请号：US15357685

申请日：2016-11-21

申请人： Victor DeFilippis ,  Tina Sali ,  Kara Pryke ,  Jinu Abraham ,  Andrew Liu ,  Iris Archer ,  Kayla Sheridan ,  Aaron Nilsen ,  Rebecca Broeckel ,  Jessica Smith ,  Lisi Amsler ,  Daniel Streblow ,  Andrew Placzek

发明人： Victor DeFilippis ,  Tina Sali ,  Kara Pryke ,  Jinu Abraham ,  Andrew Liu ,  Iris Archer ,  Kayla Sheridan ,  Aaron Nilsen ,  Rebecca Broeckel ,  Jessica Smith ,  Lisi Amsler ,  Daniel Streblow ,  Andrew Placzek

IPC分类号： G01N33/50 ,  C07D279/16 ,  C07D417/12

CPC分类号： G01N33/5041 ,  A61K31/5415 ,  C07D279/16 ,  C07D417/12 ,  G01N2333/705 ,  Y02A50/382

摘要： Disclosed are small molecules capable of activating the type I interferon (IFN) response by way of the transcription factor IFN regulatory factor 3 (IRF3) were identified. A high throughput in vitro screen yielded 4-(2-chloro-6-fluorobenzyl)-N-(furan-2-ylmethyl)-3-oxo-3,4-dihydro-2H-benzo[b][1,4]thiazine-6-carboxamide (referred to herein as G10), which was found to trigger IRF3/IFN-associated transcription in human fibroblasts. To define cellular proteins essential to elicitation of the antiviral activity by the compound a reverse genetics approach that utilized genome editing via CRISPR/Cas9 technology was employed. This allowed the identification of IRF3, the IRF3-activating adaptor molecule STING, and the IFN-associated transcription factor STAT1 as required for observed gene induction and antiviral effects.

公开(公告)号：US06225048B1

公开(公告)日：2001-05-01

申请号：US09164221

申请日：1998-09-30

申请人： Cecilia E. Soderberg-Naucler ,  Kenneth N. Fish ,  Ashlee Moses ,  Daniel Streblow ,  Jay Nelson

发明人： Cecilia E. Soderberg-Naucler ,  Kenneth N. Fish ,  Ashlee Moses ,  Daniel Streblow ,  Jay Nelson

IPC分类号： C12Q170

CPC分类号： C07K16/2833 ,  C07K2317/76 ,  C12N5/0645 ,  C12N7/00 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/24 ,  C12N2501/25 ,  C12N2502/11 ,  C12N2503/00 ,  C12N2710/16651

摘要： The present invention provides methods of latent virus reactivation in monocyte-derived macrophages through allogeneic stimulation of peripheral blood mononuclear cells (“PBMC”), methods of culturing virus, and cultures of virally permissive monocyte-derived macrophages.

摘要翻译： 本发明通过外周血单核细胞（“PBMC”）的同种异体刺激，培养病毒的方法和病毒性单核细胞衍生的巨噬细胞的培养物提供了在单核细胞衍生的巨噬细胞中潜伏病毒再活化的方法。