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1.发明申请METAL BINDING COMPOUNDS AND THEIR USE IN CELL CULTURE MEDIUM COMPOSITIONS 审中-公开金属结合化合物及其在细胞培养基组合物中的应用公开(公告)号：US20110183375A1
公开(公告)日：2011-07-28
申请号：US13013629
申请日：2011-01-25
CPC分类号： C12N5/0037 , A61K38/00 , A61K39/00 , C12N2500/24 , C12N2500/34
摘要： The present invention is directed generally to metal binding compounds which may be added to cell culture media to replace factors required for cultivation of the cells (e.g. transferrin) which are of animal or human origin. More specifically, the invention is directed to metal binding compounds or complexes thereof comprising one or more transition element cations (such as ferrous or ferric ions), which are added to cell and tissue culture medium compositions. The metal binding compounds may be added to the media alone or may be first complexed with a transition metal ion. The invention is also directed to methods of use of such compositions, including, for example, methods for the cultivation of eukaryotic cells, particularly animal cells, in vitro. The invention also relates to compositions comprising such culture media and one or more cells, and to kits comprising one or more of the above-described compositions. The compositions of the present invention obviate the need for naturally derived metal-binding proteins, such as transferrin and ceruloplasmin, which may contain blood-borne pathogens.
摘要翻译： 本发明一般涉及金属结合化合物，其可加入到细胞培养基中以替代用于培养动物或人来源的细胞（例如转铁蛋白）所需的因子。 更具体地，本发明涉及包含一种或多种过渡元素阳离子（例如亚铁或三价铁离子）的金属结合化合物或其复合物，其被添加到细胞和组织培养基组合物中。 金属结合化合物可以单独加入到介质中，或者可以首先与过渡金属离子络合。 本发明还涉及这种组合物的使用方法，包括例如在体外培养真核细胞，特别是动物细胞的方法。 本发明还涉及包含这种培养基和一种或多种细胞的组合物，以及包含一种或多种上述组合物的试剂盒。 本发明的组合物不需要可能含有血源性病原体的天然衍生的金属结合蛋白，例如转铁蛋白和血浆铜蓝蛋白。
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公开(公告)号：US10533929B2
公开(公告)日：2020-01-14
申请号：US15527054
申请日：2016-01-05
申请人： Compagnie Generale des Etablissements Michelin , Michelin Recherche et Technique S.A. , Bradley D. Schober , Frank E. Gramling , David A. Judd
摘要： A method of using a sensor device for tire inspection is provided. Signals are received from multiples rows of sensors separated by a predetermined distance. The sensors are positioned next to the inner surface of the tire for inspection. Signals from the different rows of sensors are used to identify e.g., breaks in the reinforcements of the tire and also used to identify undesirable signals generated from vibration or jarring of the sensor device.
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公开(公告)号：US20160349149A1
公开(公告)日：2016-12-01
申请号：US15112718
申请日：2014-03-13
CPC分类号： G01M17/02 , B60C25/005 , B60C25/0548 , G01M5/0033 , G01M5/0091 , G01N27/82 , G01R33/072
摘要： A sensor device is provided for use in tire inspection along a bead portion of the tire. The sensor device includes a magnet array configured to provide the desired fields of magnet flux for a sensor array, which is used to detect damage to reinforcements of a body ply of the tire near the bead portion. The fields of magnetic flux are sufficient to provide for damage detection in the bead portion without overly saturating the sensor array. The sensor device also allows for positioning the sensor array proximate to the inner surface of the tire for improved detection.
摘要翻译： 提供传感器装置，用于沿着轮胎的胎圈部分进行轮胎检查。 传感器装置包括磁体阵列，该磁体阵列被配置为提供用于传感器阵列的所需磁场磁场，该传感器阵列用于检测胎圈部分附近的胎体的增强件的损伤。 磁通量的场足以在珠部分中提供损伤检测，而不会使传感器阵列过度饱和。 传感器装置还允许将传感器阵列靠近轮胎的内表面定位，以便改进检测。
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公开(公告)号：US10006835B2
公开(公告)日：2018-06-26
申请号：US15112718
申请日：2014-03-13
CPC分类号： G01M17/02 , B60C25/005 , B60C25/0548 , G01M5/0033 , G01M5/0091 , G01N27/82 , G01R33/072
摘要： A sensor device is provided for use in tire inspection along a bead portion of the tire. The sensor device includes a magnet array configured to provide the desired fields of magnet flux for a sensor array, which is used to detect damage to reinforcements of a body ply of the tire near the bead portion. The fields of magnetic flux are sufficient to provide for damage detection in the bead portion without overly saturating the sensor array. The sensor device also allows for positioning the sensor array proximate to the inner surface of the tire for improved detection.
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公开(公告)号：US20160377507A1
公开(公告)日：2016-12-29
申请号：US15112731
申请日：2014-03-13
申请人： Bradley D. SCHOBER , Frank E. GRAMLING , David A. JUDD , COMPAGNIE GENERALE DES ETABLISSEMENTS MICHELIN , MICHELIN RECHERCHE ET TECHNIQUE, S.A.
IPC分类号： G01M17/02
CPC分类号： G01M17/02 , B60C25/005 , B60C25/0548
摘要： A sensor device is provided for use in tire inspection. The sensor device is configured for removable placement along the inner surface of a tire. The sensor device has a profile that allows placement of one or more sensors in close proximity to the inner surface of the tire. The profile allows the sensor device to be used with a variety of tire sizes and shapes.
摘要翻译： 提供用于轮胎检查的传感器装置。 传感器装置构造成沿着轮胎的内表面可移动放置。 传感器装置具有允许将一个或多个传感器放置在紧邻轮胎内表面的轮廓。 轮廓允许传感器装置使用各种轮胎尺寸和形状。
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6.发明授权公开(公告)号：US06767741B1
公开(公告)日：2004-07-27
申请号：US09650339
申请日：2000-08-28
IPC分类号： C12N500
CPC分类号： C12N5/0037 , A61K38/00 , A61K39/00 , C12N2500/24 , C12N2500/34
摘要： The present invention is directed generally to metal binding compounds which may be added to cell culture media to replace factors required for cultivation of the cells (e.g. transferrin) which are of animal or human origin. More specifically, the invention is directed to metal binding compounds or complexes thereof comprising one or more transition element cations (such as ferrous or ferric ions), which are added to cell and tissue culture medium compositions. The metal binding compounds may be added to the media alone or may be first complexed with a transition metal ion. The invention is also directed to methods of use of such compositions, including, for example, methods for the cultivation of eukaryotic cells, particularly animal cells, in vitro. The invention also relates to compositions comprising such culture media and one or more cells, and to kits comprising one or more of the above-describcd compositions. The compositions of the present invention obviate the need for naturally derived metal-binding proteins, such as transferrin and ceruloplasmin, which may contain blood-borne pathogens.
摘要翻译： 本发明一般涉及金属结合化合物，其可加入到细胞培养基中以替代用于培养动物或人来源的细胞（例如转铁蛋白）所需的因子。 更具体地，本发明涉及包含一种或多种过渡元素阳离子（例如亚铁或三价铁离子）的金属结合化合物或其复合物，其被添加到细胞和组织培养基组合物中。 金属结合化合物可以单独加入到介质中，或者可以首先与过渡金属离子络合。 本发明还涉及这种组合物的使用方法，包括例如在体外培养真核细胞，特别是动物细胞的方法。 本发明还涉及包含这种培养基和一种或多种细胞的组合物，以及包含上述一种或多种上述组合物的试剂盒。 本发明的组合物不需要可能含有血源性病原体的天然衍生的金属结合蛋白，例如转铁蛋白和血浆铜蓝蛋白。
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公开(公告)号：US10712402B2
公开(公告)日：2020-07-14
申请号：US15527051
申请日：2016-01-05
摘要： A sensor device is provided for use in tire inspection. The sensor device is configured for removable placement along the inner surface of the tire. The sensor device includes multiple rows of sensors, which are used to provide signals that can compensate for the effect of vibrations or mechanical agitation as the sensors are passed over the inner surface of the tire. The sensor device may have a profile that allows for placement of the multiple rows of sensors in close proximity to the inner surface of the tire.
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8.发明申请METAL BINDING COMPOUNDS AND THEIR USE IN CELL CULTURE MEDIUM COMPOSITIONS 审中-公开金属结合化合物及其在细胞培养基组合物中的应用公开(公告)号：US20080286868A1
公开(公告)日：2008-11-20
申请号：US12135926
申请日：2008-06-09
IPC分类号： C12N5/02
CPC分类号： C12N5/0037 , A61K38/00 , A61K39/00 , C12N2500/24 , C12N2500/34
摘要： The present invention is directed generally to metal binding compounds which may be added to cell culture media to replace factors required for cultivation of the cells (e.g. transferrin) which are of animal or human origin. More specifically, the invention is directed to metal binding compounds or complexes thereof comprising one or more transition element cations (such as ferrous or ferric ions), which are added to cell and tissue culture medium compositions. The metal binding compounds may be added to the media alone or may be first complexed with a transition metal ion. The invention is also directed to methods of use of such compositions, including, for example, methods for the cultivation of eukaryotic cells, particularly animal cells, in vitro. The invention also relates to compositions comprising such culture media and one or more cells, and to kits comprising one or more of the above-described compositions. The compositions of the present invention obviate the need for naturally derived metal-binding proteins, such as transferrin and ceruloplasmin, which may contain blood-borne pathogens.
摘要翻译： 本发明一般涉及金属结合化合物，其可加入到细胞培养基中以替代用于培养动物或人来源的细胞（例如转铁蛋白）所需的因子。 更具体地，本发明涉及包含一种或多种过渡元素阳离子（例如亚铁或三价铁离子）的金属结合化合物或其复合物，其被添加到细胞和组织培养基组合物中。 金属结合化合物可以单独加入到介质中，或者可以首先与过渡金属离子络合。 本发明还涉及这种组合物的使用方法，包括例如在体外培养真核细胞，特别是动物细胞的方法。 本发明还涉及包含这种培养基和一种或多种细胞的组合物，以及包含一种或多种上述组合物的试剂盒。 本发明的组合物不需要可能含有血源性病原体的天然衍生的金属结合蛋白，例如转铁蛋白和血浆铜蓝蛋白。
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公开(公告)号：US06692961B1
公开(公告)日：2004-02-17
申请号：US09695926
申请日：2000-10-26
申请人： David A. Judd , Paul J. Battista
发明人： David A. Judd , Paul J. Battista
IPC分类号： C12N500
CPC分类号： C12N5/0629 , C12N2500/30 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/42 , C12N2500/90 , C12N2501/01 , C12N2501/113
摘要： The present invention provides cell culture media formulations which support the in vitro cultivation of animal epithelial cells. The media comprise at least one fibroblast growth factor (FGF) and at least one agent that induces increased intracellular cAMP levels, and optionally comprise ascorbic acid. The present invention also provides methods of cultivating animal epithelial cells in vitro using these cell culture media formulations, kits comprising the media, cell culture compositions comprising the culture media and an animal epithelial cell, and compositions that may be used as replacements for organ or gland extracts in animal cell culture media.
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