公开(公告)号：US6162341A

公开(公告)日：2000-12-19

申请号：US151928

申请日：1998-09-11

申请人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

发明人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

IPC分类号： G01N27/447 ,  G01N35/00 ,  G01N27/26

CPC分类号： G01N27/44782 ,  G01N2035/00237

摘要： A multi-channel capillary electrophoresis apparatus is disclosed. The apparatus includes a capillary array assembly comprising a plurality of capillaries, each capillary having a capillary outlet, and an outlet support for supporting the capillary outlets. The apparatus further includes a cuvette defining a receiving slot, a gap region, and a detection zone, where the receiving slot is adapted to removably receive the outlet support, and wherein when the outlet support is inserted into the receiving slot, the capillary outlets are positioned in the gap region in proximity to the detection zone, and a flow channel is formed by the outlet support and the receiving slot such that the flow channel is in fluid communication with the gap region. In addition, the apparatus includes a front plumbing block in fluid communication with the flow channel for supplying a fluid flow through the gap region sufficient to transport material downstream from the capillary outlets to the detection zone.

摘要翻译： 公开了一种多通道毛细管电泳装置。 该装置包括毛细管阵列组件，其包括多个毛细管，每个毛细管具有毛细管出口，以及用于支撑毛细管出口的出口支撑件。 所述设备还包括限定接收槽，间隙区和检测区的比色皿，其中所述接收槽适于可移除地接收所述出口支撑，并且其中当所述出口支撑件插入所述接收槽时，所述毛细管出口是 定位在接近检测区域的间隙区域中，并且流动通道由出口支撑件和接收槽形成，使得流动通道与间隙区域流体连通。 此外，该装置包括与流动通道流体连通的前管道块，用于提供足以将材料从毛细管出口下游输送到检测区域的间隙区域的流体流。

公开(公告)号：US5372695A

公开(公告)日：1994-12-13

申请号：US59946

申请日：1993-05-07

申请人： David M. Demorest

发明人： David M. Demorest

IPC分类号： G01N27/447 ,  B01D61/42

CPC分类号： G01N27/44704

摘要： A capillary electrophoresis system incorporates an application specific cassette having run buffers and capillary column selected for the specific separation procedure, with buffer reservoirs sealed for storage and shipment. A serving apparatus is provided to accept the cassette and perform the specific electrophoresis procedure until buffer is depleted or the performance of the separation column deteriorates. The serving apparatus comprises liquid transfer apparatus, removable covers for the reservoirs of the cassette, electrodes for establishing voltage potential, and a control system.

摘要翻译： 毛细管电泳系统包含具有运行缓冲液和针对特定分离程序选择的毛细管柱的专用盒，其中缓冲液储存器被密封用于储存和运输。 提供服务装置以接受盒并执行特定电泳过程，直到缓冲液耗尽或分离柱的性能劣化。 服务设备包括液体传送装置，用于盒的储存器的可移除盖，用于建立电压电位的电极和控制系统。

公开(公告)号：US06297009B1

公开(公告)日：2001-10-02

申请号：US09005075

申请日：1998-01-09

申请人： David M. Demorest ,  Stephen E. Moring ,  Claudia Chiesa

发明人： David M. Demorest ,  Stephen E. Moring ,  Claudia Chiesa

IPC分类号： C12Q168

CPC分类号： G01N27/44752 ,  G01N30/6052 ,  G01N2030/285

摘要： Disclosed is a method for increasing the electroosmotic flow rate available for a silica surface. In the method, there is provided an electrophoretic channel which is defined by one or more silica surfaces. The surface(s) are contacted with an alkaline aqueous solution containing a solubilized silicate-monovalent metal complex in an amount effective to increase the acidity of the silica surface(s), as evidenced by a reduction in the average bulk pKa of the surface(s). The achieved increase in acidity is greater than would be obtained using an otherwise identical solution lacking said silicate. In one preferred embodiment, the monovalent metal used in the solution is Li+, Na+, or K+. Also disclosed is a method for increasing the acidity of a silica surface, by contacting the surface with an alkaline aqueous solution of the type noted above.

摘要翻译： 公开了一种增加可用于二氧化硅表面的电渗流速的方法。 在该方法中，提供了由一个或多个二氧化硅表面限定的电泳通道。 表面与含有增溶的硅酸盐 - 一价金属络合物的碱性水溶液以有效提高二氧化硅表面酸度的量接触，这通过表面平均体积pKa的降低来证明（ s）。 实现的酸度增加大于使用其它相同的不含所述硅酸盐的溶液所获得的增加。 在一个优选的实施方案中，溶液中使用的一价金属是Li +，Na +或K +。 还公开了通过使表面与上述类型的碱性水溶液接触来提高二氧化硅表面的酸度的方法。

公开(公告)号：US5578179A

公开(公告)日：1996-11-26

申请号：US501674

申请日：1995-07-12

申请人： David M. Demorest ,  Stephen E. Moring ,  Claudia Chiesa

发明人： David M. Demorest ,  Stephen E. Moring ,  Claudia Chiesa

IPC分类号： G01N27/447 ,  G01N30/28 ,  G01N30/60 ,  G01N27/26

CPC分类号： G01N27/44752 ,  G01N2030/285 ,  G01N30/6052

摘要： Disclosed is a method for increasing the electro-osmotic flow rate available for a silica surface. In the method, there is provided an electrophoretic channel which is defined by one or more silica surfaces. The surface(s) are contacted with an alkaline aqueous solution containing a solubilized silicate-monovalent metal complex in an amount effective to increase the acidity of the silica surface(s), as evidenced by a reduction in the average bulk pKa of the surface(s). The achieved increase in acidity is greater than would be obtained using an otherwise identical solution lacking said silicate. In one preferred embodiment, the monovalent metal used in the solution is Li.sup.+, Na.sup.+, or K.sup.+. Also disclosed is a method for increasing the acidity of a silica surface, by contacting the surface with an alkaline aqueous solution of the type noted above.

摘要翻译： 公开了一种增加可用于二氧化硅表面的电渗流速的方法。 在该方法中，提供了由一个或多个二氧化硅表面限定的电泳通道。 表面与含有增溶的硅酸盐 - 一价金属络合物的碱性水溶液以有效提高二氧化硅表面酸度的量接触，这通过表面平均体积pKa的降低来证明（ s）。 实现的酸度增加大于使用其它相同的不含所述硅酸盐的溶液所获得的增加。 在一个优选的实施方案中，溶液中使用的一价金属是Li +，Na +或K +。 还公开了通过使表面与上述类型的碱性水溶液接触来提高二氧化硅表面的酸度的方法。

公开(公告)号：US06387236B2

公开(公告)日：2002-05-14

申请号：US09805624

申请日：2001-03-13

申请人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

发明人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

IPC分类号： G01N2726

CPC分类号： G01N27/44782 ,  G01N2035/00237

摘要： A multi-channel capillary electrophoresis apparatus is disclosed. The apparatus includes a capillary array assembly comprising a plurality of capillaries, each capillary having a capillary outlet, and an outlet support for supporting the capillary outlets. The apparatus further includes a cuvette defining a receiving slot, a gap region, and a detection zone, where the receiving slot is adapted to removably receive the outlet support, and wherein when the outlet support is inserted into the receiving slot, the capillary outlets are positioned in the gap region in proximity to the detection zone, and a flow channel is formed by the outlet support and the receiving slot such that the flow channel is in fluid communication with the gap region. In addition, the apparatus includes a front plumbing block in fluid communication with the flow channel for supplying a fluid flow through the gap region sufficient to transport material downstream from the capillary outlets to the detection zone.

摘要翻译： 公开了一种多通道毛细管电泳装置。 该装置包括毛细管阵列组件，其包括多个毛细管，每个毛细管具有毛细管出口，以及用于支撑毛细管出口的出口支撑件。 所述设备还包括限定接收槽，间隙区和检测区的比色皿，其中所述接收槽适于可移除地接收所述出口支撑，并且其中当所述出口支撑件插入所述接收槽时，所述毛细管出口是 定位在接近检测区域的间隙区域中，并且流动通道由出口支撑件和接收槽形成，使得流动通道与间隙区域流体连通。 此外，该装置包括与流动通道流体连通的前管道块，用于提供足以将材料从毛细管出口下游输送到检测区域的间隙区域的流体流。

公开(公告)号：US06231739B1

公开(公告)日：2001-05-15

申请号：US09621071

申请日：2000-07-21

申请人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

发明人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

IPC分类号： G01N2726

CPC分类号： G01N27/44782 ,  G01N2035/00237

摘要： A multi-channel capillary electrophoresis apparatus is disclosed. The apparatus includes a capillary array assembly comprising a plurality of capillaries, each capillary having a capillary outlet, and an outlet support for supporting the capillary outlets. The apparatus further includes a cuvette defining a receiving slot, a gap region, and a detection zone, where the receiving slot is adapted to removably receive the outlet support, and wherein when the outlet support is inserted into the receiving slot, the capillary outlets are positioned in the gap region in proximity to the detection zone, and a flow channel is formed by the outlet support and the receiving slot such that the flow channel is in fluid communication with the gap region. In addition, the apparatus includes a front plumbing block in fluid communication with the flow channel for supplying a fluid flow through the gap region sufficient to transport material downstream from the capillary outlets to the detection zone.

摘要翻译： 公开了一种多通道毛细管电泳装置。 该装置包括毛细管阵列组件，其包括多个毛细管，每个毛细管具有毛细管出口，以及用于支撑毛细管出口的出口支撑件。 所述设备还包括限定接收槽，间隙区和检测区的比色皿，其中所述接收槽适于可移除地接收所述出口支撑，并且其中当所述出口支撑件插入所述接收槽时，所述毛细管出口是 定位在接近检测区域的间隙区域中，并且流动通道由出口支撑件和接收槽形成，使得流动通道与间隙区域流体连通。 此外，该装置包括与流动通道流体连通的前管道块，用于提供足以将材料从毛细管出口下游输送到检测区域的间隙区域的流体流。

公开(公告)号：US5264101A

公开(公告)日：1993-11-23

申请号：US877956

申请日：1992-05-01

申请人： David M. Demorest ,  William E. Werner ,  John E. Wiktorowicz

发明人： David M. Demorest ,  William E. Werner ,  John E. Wiktorowicz

IPC分类号： G01N33/68 ,  B01D57/02 ,  C07H21/04 ,  C07K1/26 ,  C08F20/52 ,  C08F26/00 ,  C08F220/56 ,  C08F226/02 ,  C08L33/24 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44752 ,  G01N27/44747

摘要： A capillary tube with a charged inner surface for use in capillary electrophoresis and methods. The tube is filled with an electrolyte solution containing 0.05-30% weight/weight of a hydrophilic polymer which is characterized by (A) a molecular weight of 20-5,000 kilodaltons, and (B) a charge between 0.01 and 1.0% as measured by the molar percent of charged monomer subunits to the total polymer subunits, where said charged monomer subunits have the charge opposite to the wall charge at a selected electrophoresis pH.

摘要翻译： 具有带电内表面的毛细管用于毛细管电泳和方法。 该管充满电解质溶液，该溶液含有0.05-30％重量的亲水性聚合物，其特征在于（A）分子量为20-5,000千道尔顿，和（B）0.01至1.0％的电荷，如 带电单体亚基与总聚合物亚基的摩尔百分数，其中所述带电单体亚单位在选定的电泳pH下具有与壁电荷相反的电荷。