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    Multi-channel capillary electrophoresis device including sheath-flow cuvette and replacable capillary array
    1.
    发明授权
    Multi-channel capillary electrophoresis device including sheath-flow cuvette and replacable capillary array 失效
    多通道毛细管电泳装置，包括鞘流比色杯和可更换的毛细管阵列

    公开(公告)号：US6162341A

    公开(公告)日：2000-12-19

    申请号：US151928

    申请日：1998-09-11

    申请人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

    发明人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

    IPC分类号： G01N27/447 ,  G01N35/00 ,  G01N27/26

    CPC分类号： G01N27/44782 ,  G01N2035/00237

    摘要： A multi-channel capillary electrophoresis apparatus is disclosed. The apparatus includes a capillary array assembly comprising a plurality of capillaries, each capillary having a capillary outlet, and an outlet support for supporting the capillary outlets. The apparatus further includes a cuvette defining a receiving slot, a gap region, and a detection zone, where the receiving slot is adapted to removably receive the outlet support, and wherein when the outlet support is inserted into the receiving slot, the capillary outlets are positioned in the gap region in proximity to the detection zone, and a flow channel is formed by the outlet support and the receiving slot such that the flow channel is in fluid communication with the gap region. In addition, the apparatus includes a front plumbing block in fluid communication with the flow channel for supplying a fluid flow through the gap region sufficient to transport material downstream from the capillary outlets to the detection zone.

    摘要翻译： 公开了一种多通道毛细管电泳装置。 该装置包括毛细管阵列组件，其包括多个毛细管，每个毛细管具有毛细管出口，以及用于支撑毛细管出口的出口支撑件。 所述设备还包括限定接收槽，间隙区和检测区的比色皿，其中所述接收槽适于可移除地接收所述出口支撑，并且其中当所述出口支撑件插入所述接收槽时，所述毛细管出口是 定位在接近检测区域的间隙区域中，并且流动通道由出口支撑件和接收槽形成，使得流动通道与间隙区域流体连通。 此外，该装置包括与流动通道流体连通的前管道块，用于提供足以将材料从毛细管出口下游输送到检测区域的间隙区域的流体流。

    Multi-channel capillary electrophoresis device including sheath-flow cuvette and replaceable capillary array
    2.
    发明授权
    Multi-channel capillary electrophoresis device including sheath-flow cuvette and replaceable capillary array 失效
    多通道毛细管电泳装置，包括鞘流比色皿和可更换的毛细管阵列

    公开(公告)号：US06387236B2

    公开(公告)日：2002-05-14

    申请号：US09805624

    申请日：2001-03-13

    申请人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

    发明人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

    IPC分类号： G01N2726

    CPC分类号： G01N27/44782 ,  G01N2035/00237

    摘要： A multi-channel capillary electrophoresis apparatus is disclosed. The apparatus includes a capillary array assembly comprising a plurality of capillaries, each capillary having a capillary outlet, and an outlet support for supporting the capillary outlets. The apparatus further includes a cuvette defining a receiving slot, a gap region, and a detection zone, where the receiving slot is adapted to removably receive the outlet support, and wherein when the outlet support is inserted into the receiving slot, the capillary outlets are positioned in the gap region in proximity to the detection zone, and a flow channel is formed by the outlet support and the receiving slot such that the flow channel is in fluid communication with the gap region. In addition, the apparatus includes a front plumbing block in fluid communication with the flow channel for supplying a fluid flow through the gap region sufficient to transport material downstream from the capillary outlets to the detection zone.

    摘要翻译： 公开了一种多通道毛细管电泳装置。 该装置包括毛细管阵列组件，其包括多个毛细管，每个毛细管具有毛细管出口，以及用于支撑毛细管出口的出口支撑件。 所述设备还包括限定接收槽，间隙区和检测区的比色皿，其中所述接收槽适于可移除地接收所述出口支撑，并且其中当所述出口支撑件插入所述接收槽时，所述毛细管出口是 定位在接近检测区域的间隙区域中，并且流动通道由出口支撑件和接收槽形成，使得流动通道与间隙区域流体连通。 此外，该装置包括与流动通道流体连通的前管道块，用于提供足以将材料从毛细管出口下游输送到检测区域的间隙区域的流体流。

    Multi-channel capillary electrophoresis device including sheath-flow cuvette and replacable capillary array
    3.
    发明授权
    Multi-channel capillary electrophoresis device including sheath-flow cuvette and replacable capillary array 有权
    多通道毛细管电泳装置，包括鞘流比色杯和可更换的毛细管阵列

    公开(公告)号：US06231739B1

    公开(公告)日：2001-05-15

    申请号：US09621071

    申请日：2000-07-21

    申请人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

    发明人： Eric S. Nordman ,  John Shigeura ,  Albert L. Carrillo ,  David M. Demorest ,  Philip J. Wunderle

    IPC分类号： G01N2726

    CPC分类号： G01N27/44782 ,  G01N2035/00237

    摘要： A multi-channel capillary electrophoresis apparatus is disclosed. The apparatus includes a capillary array assembly comprising a plurality of capillaries, each capillary having a capillary outlet, and an outlet support for supporting the capillary outlets. The apparatus further includes a cuvette defining a receiving slot, a gap region, and a detection zone, where the receiving slot is adapted to removably receive the outlet support, and wherein when the outlet support is inserted into the receiving slot, the capillary outlets are positioned in the gap region in proximity to the detection zone, and a flow channel is formed by the outlet support and the receiving slot such that the flow channel is in fluid communication with the gap region. In addition, the apparatus includes a front plumbing block in fluid communication with the flow channel for supplying a fluid flow through the gap region sufficient to transport material downstream from the capillary outlets to the detection zone.

    摘要翻译： 公开了一种多通道毛细管电泳装置。 该装置包括毛细管阵列组件，其包括多个毛细管，每个毛细管具有毛细管出口，以及用于支撑毛细管出口的出口支撑件。 所述设备还包括限定接收槽，间隙区和检测区的比色皿，其中所述接收槽适于可移除地接收所述出口支撑，并且其中当所述出口支撑件插入所述接收槽时，所述毛细管出口是 定位在接近检测区域的间隙区域中，并且流动通道由出口支撑件和接收槽形成，使得流动通道与间隙区域流体连通。 此外，该装置包括与流动通道流体连通的前管道块，用于提供足以将材料从毛细管出口下游输送到检测区域的间隙区域的流体流。

    Sample handling system for a multi-channel capillary electrophoresis device
    4.
    发明授权
    Sample handling system for a multi-channel capillary electrophoresis device 有权
    多通道毛细管电泳装置的样品处理系统

    公开(公告)号：US07169279B2

    公开(公告)日：2007-01-30

    申请号：US10288405

    申请日：2002-11-04

    申请人： Howard Gregg King ,  John Shigeura ,  Eric S. Nordman ,  Sean Matthew Desmond

    发明人： Howard Gregg King ,  John Shigeura ,  Eric S. Nordman ,  Sean Matthew Desmond

    IPC分类号： G01N27/447

    CPC分类号： G01N27/44743

    摘要： A sample handling system in a multi-channel capillary electrophoresis apparatus is disclosed. The sample handling system includes a work surface for supporting a plurality of samples located at a plurality of work surface coordinates and a sample loading assembly comprising a plurality of loading wells. At least one of the loading wells includes a capillary fixedly positioned therein. The system further includes a programmable sample transfer device for automatically transferring a sample from a work surface coordinate to a loading well. The invention further includes methods for using the sample handling system.

    摘要翻译： 公开了一种多通道毛细管电泳装置中的样品处理系统。 样品处理系统包括用于支撑位于多个工作表面坐标处的多个样品的工作表面和包括多个加载孔的样品加载组件。 至少一个装载井包括固定地定位在其中的毛细管。 该系统还包括用于将样品从工作面坐标自动转移到装载井的可编程样品转移装置。 本发明还包括使用样品处理系统的方法。

    Time-delay integration in electrophoretic detection systems
    6.
    发明授权
    Time-delay integration in electrophoretic detection systems 有权

    公开(公告)号：US06856390B2

    公开(公告)日：2005-02-15

    申请号：US10205028

    申请日：2002-07-25

    申请人： Eric S. Nordman ,  Richard T. Reel ,  John Shigeura

    发明人： Eric S. Nordman ,  Richard T. Reel ,  John Shigeura

    IPC分类号： G01N21/64 ,  G01N27/447 ,  G01N21/00

    CPC分类号： G01N27/44721

    摘要： An apparatus for detecting analytes in a sample is provided. The apparatus includes: one or more channels having a detection zone; one or more irradiation sources disposed for irradiating the detection zone with non-coherent radiation; a detector array disposed for collecting light signals emitted from markers in the detection zone excited by the radiation, the detector array having an output; and a system coupled to the detector array for effecting time delay integration of the charges on the detector array corresponding to the light signals by accumulating the charges before reading the charges at the output of the detector array. Other apparatus and methods for detecting analytes in a sample are also provided.

    Fluid Manipulation Assembly
    7.
    发明申请
    Fluid Manipulation Assembly 审中-公开
    流体操纵装置

    公开(公告)号：US20090258415A1

    公开(公告)日：2009-10-15

    申请号：US12490219

    申请日：2009-06-23

    申请人： Zbigniew T. Bryning ,  John Shigeura

    发明人： Zbigniew T. Bryning ,  John Shigeura

    IPC分类号： C12M1/34

    CPC分类号： B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683

    摘要： Microfluidic assemblies, systems, and methods are provided for manipulating fluid samples. Assemblies include an elastically deformable cover layer and a less elastically deformable substrate. The methods include deforming the substrate through the cover layer so that when the cover layer rebounds a new communication results in the assembly between the cover layer and the substrate and/or so that a new barrier wall is formed. Systems for carrying out the methods are also provided.

    摘要翻译： 提供微流体组件，系统和方法来操作流体样品。 组件包括可弹性变形的覆盖层和较少可弹性变形的衬底。 所述方法包括通过覆盖层使基底变形，使得当覆盖层反弹新的通信导致覆盖层和基底之间的组装和/或使得形成新的阻挡壁时。 还提供了执行方法的系统。

    System for real time detection of nucleic acid amplification products
    9.
    发明授权
    System for real time detection of nucleic acid amplification products 失效
    核酸扩增产物实时检测系统

    公开(公告)号：US5928907A

    公开(公告)日：1999-07-27

    申请号：US752973

    申请日：1996-12-02

    申请人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

    发明人： Timothy M. Woudenberg ,  Kevin S. Bodner ,  Charles R. Connell ,  Alan M. Ganz ,  Lincoln J. McBride ,  Paul G. Saviano ,  John Shigeura ,  David H. Tracy ,  Eugene F. Young ,  Linda G. Lee

    IPC分类号： C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/03 ,  G01N21/64 ,  G01N21/75 ,  G01N21/78 ,  G01N33/50 ,  G01N33/58

    CPC分类号： G01N21/6428 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/0303 ,  G01N2021/6484

    摘要： A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicator capable of generating a first fluorescent signal whose intensity is proportional to the amount of an amplification product in the volume of the reaction mixture illuminated by the excitation beam and (ii) a second fluorescent indicator homogeneously distributed throughout the reaction mixture capable of generating a second fluorescent signal proportional to the volume of reaction mixture illuminated by the excitation beam. Preferably, the excitation beam is focused into the reaction mixture by a lens through a portion of a wall of a closed reaction chamber containing the reaction mixture. The same lens is used to collect the first and second fluorescent signals generated by the first and second fluorescent indicators, respectively, in response to the excitation beam. The ratio of the fluorescent intensities of the first and second fluorescent signals provides a stable quantitative indicator of the amount of amplification product synthesized in the course of the amplification reaction.

    摘要翻译： 提供了一种用于进行核酸扩增产物的实时基于荧光的测量的系统。 在本发明的优选实施方案中，激发光束通过表面聚焦到反应混合物中，反应混合物含有（i）第一荧光指示剂，其能够产生第一荧光信号，其强度与扩增产物的量成比例 在由激发束照射的反应混合物的体积中和（ii）均匀分布在整个反应混合物中的第二荧光指示器，其能够产生与由激发束照射的反应混合物的体积成比例的第二荧光信号。 优选地，激发光束通过透镜通过包含反应混合物的封闭反应室的壁的一部分聚焦到反应混合物中。 相同的透镜被用于响应于激发光束分别收集由第一和第二荧光指示器产生的第一和第二荧光信号。 第一和第二荧光信号的荧光强度比提供了在扩增反应过程中合成的扩增产物的量的稳定的定量指标。