公开(公告)号：US6132713A

公开(公告)日：2000-10-17

申请号：US271713

申请日：1999-03-18

申请人： David Ray Fiipula ,  Maoliang Wang

发明人： David Ray Fiipula ,  Maoliang Wang

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/46 ,  A61K38/50 ,  A61K47/48 ,  A61P35/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/78 ,  C12N11/08 ,  C12N11/10 ,  C12R1/35 ,  A61K38/54 ,  C12N9/14

CPC分类号： A61K38/50 ,  C12N11/08 ,  C12N11/10 ,  C12N9/78

摘要： A purified arginine deiminase (ADI) obtained from Mycoplasma arthritidis having the amino acid sequence of SEQ ID NO:2 as well as an isolated nucleic acid molecule containing a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:1 are disclosed. Other aspects of the invention include an expression vector, a cloned gene for expressing the Mycoplasma arthritidis derived ADI, (recombinant) host cells useful in expressing the ADI of the present invention and substantially non-antigenic polymer conjugates containing the ADI of the present invention as well as methods of treating arginine deiminase susceptible conditions in mammals. The arginine deiminase-polymer conjugates have high levels of retained enzyme activity and relatively long circulating lives.

摘要翻译： 公开了从具有SEQ ID NO：2的氨基酸序列的关节炎支原体获得的纯化精氨酸脱亚氨酶（ADI）以及含有编码SEQ ID NO：1所示的氨基酸序列的核苷酸序列的分离的核酸分子。 本发明的其它方面包括表达载体，用于表达来源于关节炎支原体的ADI的克隆基因，用于表达本发明的ADI的重组宿主细胞和含有本发明的ADI的基本上非抗原性的聚合物缀合物，作为 以及在哺乳动物中治疗精氨酸脱亚胺酶易感条件的方法。 精氨酸脱亚氨酶 - 聚合物共轭物具有高水平的保留酶活性和较长的循环寿命。