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    Recombinant host for producing L-asparaginase II
    1.
    发明授权
    Recombinant host for producing L-asparaginase II 有权
    用于生产L-天冬酰胺酶II的重组宿主

    公开(公告)号：US07807436B2

    公开(公告)日：2010-10-05

    申请号：US11759988

    申请日：2007-06-08

    申请人： David Ray Filpula ,  Maoliang Wang

    发明人： David Ray Filpula ,  Maoliang Wang

    IPC分类号： C12P21/00 ,  C12N15/00 ,  C12N1/21 ,  C07H21/00 ,  C12N9/82 ,  C12Q1/34 ,  C07K14/00

    CPC分类号： C12N9/82 ,  A61K38/00 ,  C12N9/96 ,  Y02A50/473

    摘要： The invention provides a recombinant Escherichia coli host cell for producing an Escherichia coli-asparaginase II enzyme. The host cell includes an Escherichia coli chromosome and at least one copy of a recombinant extrachromosomal vector, wherein the recombinant extrachromosomal vector encodes the L-asparaginase II enzyme, wherein the host cell chromosome also encodes the same L-asparaginase II enzyme, and wherein the host chromosome does not encode any other isoform of L-asparaginase II.

    摘要翻译： 本发明提供了用于产生大肠杆菌 - 天冬酰胺酶II酶的重组大肠杆菌宿主细胞。 宿主细胞包括大肠杆菌染色体和重组染色体外载体的至少一个拷贝，其中重组染色体外载体编码L-天冬酰胺酶II酶，其中宿主细胞染色体还编码相同的L-天冬酰胺酶II酶，其中 宿主染色体不编码L-天冬酰胺酶II的任何其他同种型。

    RECOMBINANT HOST FOR PRODUCING L-ASPARAGINASE II
    2.
    发明申请
    RECOMBINANT HOST FOR PRODUCING L-ASPARAGINASE II 有权
    用于生产L-阿糖胞苷酶II的重组宿主

    公开(公告)号：US20080063632A1

    公开(公告)日：2008-03-13

    申请号：US11759988

    申请日：2007-06-08

    申请人： David Filpula ,  Maoliang Wang

    发明人： David Filpula ,  Maoliang Wang

    IPC分类号： A61K38/50 ,  A61P35/00 ,  C07H21/04 ,  C12N1/21 ,  C12N15/63 ,  C12N9/82 ,  C12P1/04

    CPC分类号： C12N9/82 ,  A61K38/00 ,  C12N9/96 ,  Y02A50/473

    摘要： The invention provides a recombinant Escherichia coli host cell for producing an Escherichia coli-asparaginase II enzyme. The host cell includes an Escherichia coli chromosome and at least one copy of a recombinant extrachromosomal vector, wherein the recombinant extrachromosomal vector encodes the L-asparaginase II enzyme, wherein the host cell chromosome also encodes the same L-asparaginase II enzyme, and wherein the host chromosome does not encode any other isoform of L-asparaginase II.

    摘要翻译： 本发明提供了用于产生大肠杆菌 - 天冬酰胺酶II酶的重组大肠杆菌宿主细胞。 宿主细胞包括大肠杆菌染色体和重组染色体外载体的至少一个拷贝，其中重组染色体外载体编码L-天冬酰胺酶II酶，其中宿主细胞染色体还编码相同的L-天冬酰胺酶II酶，其中 宿主染色体不编码L-天冬酰胺酶II的任何其他同种型。

    Single-chain antigen-binding proteins capable of glycosylation, production and uses thereof
    3.
    发明授权
    Single-chain antigen-binding proteins capable of glycosylation, production and uses thereof 失效
    能够进行糖基化，生产和使用的单链抗原结合蛋白

    公开(公告)号：US06323322B1

    公开(公告)日：2001-11-27

    申请号：US09069821

    申请日：1998-04-30

    申请人： David Filpula ,  Maoliang Wang ,  Robert Shorr ,  Marc Whitlow ,  Lihsyng S. Lee

    发明人： David Filpula ,  Maoliang Wang ,  Robert Shorr ,  Marc Whitlow ,  Lihsyng S. Lee

    IPC分类号： C07K1600

    CPC分类号： G01N33/6857 ,  A61K47/60 ,  A61K2039/505 ,  C07K16/30 ,  C07K19/00 ,  C07K2317/41 ,  C07K2317/622 ,  C07K2319/00

    摘要： The present invention relates to single-chain antigen-binding molecules capable of glycosylation. Compositions of, genetic constructions coding for, and methods for producing monovalent and multivalent single-chain antigen-binding molecules capable of glycosylation are described and claimed. Composition of, genetic constructions coding for, and methods for producing glycosylated monovalent and multivalent single-chain antigen-binding molecules capable of polyalkylene oxide conjugation are described and claimed. The invention also relates to methods for producing a polypeptide having increased glycosylation and the polypeptide produced by the described method. Uses resulting from the multifunctionality of a glycosylated/polyalkylene oxide conjugated antigen-binding protein are also described and claimed.

    摘要翻译： 本发明涉及能够进行糖基化的单链抗原结合分子。 描述和要求保护编码的组成，以及产生能够糖基化的单价和多价单链抗原结合分子的方法。 描述和要求保护编码的遗传结构的组成，以及能够产生聚环氧烷共轭的糖基化单价和多价单链抗原结合分子的方法。 本发明还涉及生产具有增加的糖基化的多肽的方法和通过所述方法产生的多肽。 还描述和要求保护由糖基化/聚环氧烷结合的抗原结合蛋白的多功能性产生的用途。

    RECOMBINANT HOST FOR PRODUCING L-ASPARAGINASE II
    4.
    发明申请
    RECOMBINANT HOST FOR PRODUCING L-ASPARAGINASE II 审中-公开
    用于生产L-阿糖胞苷酶II的重组宿主

    公开(公告)号：US20100273236A1

    公开(公告)日：2010-10-28

    申请号：US12773320

    申请日：2010-05-04

    申请人： David Ray FILPULA ,  Maoliang WANG

    发明人： David Ray FILPULA ,  Maoliang WANG

    IPC分类号： C12N9/82 ,  C12N1/21

    CPC分类号： C12N9/82 ,  A61K38/00 ,  C12N9/96 ,  Y02A50/473

    摘要： The invention provides a recombinant Escherichia coli host cell for producing an Escherichia coli L-asparaginase II enzyme. The host cell includes an Escherichia coli chromosome and at least one copy of a recombinant extrachromosomal vector, wherein the recombinant extrachromosomal vector encodes the L-asparaginase II enzyme, wherein the host cell chromosome also encodes the same L-asparaginase II enzyme, and wherein the host chromosome does not encode any other isoform of L-asparaginase II.

    摘要翻译： 本发明提供了用于产生大肠杆菌L-天冬酰胺酶II酶的重组大肠杆菌宿主细胞。 宿主细胞包括大肠杆菌染色体和重组染色体外载体的至少一个拷贝，其中重组染色体外载体编码L-天冬酰胺酶II酶，其中宿主细胞染色体还编码相同的L-天冬酰胺酶II酶，其中 宿主染色体不编码L-天冬酰胺酶II的任何其他同种型。

    Single-chain antigen-binding proteins capable of glycosylation, production and uses thereof
    6.
    发明授权
    Single-chain antigen-binding proteins capable of glycosylation, production and uses thereof 有权
    能够进行糖基化，生产和使用的单链抗原结合蛋白

    公开(公告)号：US06743908B2

    公开(公告)日：2004-06-01

    申请号：US09956087

    申请日：2001-09-20

    申请人： David Filpula ,  Maoliang Wang ,  Robert Shorr ,  Marc Whitlow ,  Lihsyng S. Lee

    发明人： David Filpula ,  Maoliang Wang ,  Robert Shorr ,  Marc Whitlow ,  Lihsyng S. Lee

    IPC分类号： C07H2104

    CPC分类号： G01N33/6857 ,  A61K47/60 ,  A61K2039/505 ,  C07K16/30 ,  C07K19/00 ,  C07K2317/41 ,  C07K2317/622 ,  C07K2319/00

    摘要： The present invention relates to single-chain antigen-binding molecules capable of glycosylation. Compositions of, genetic constructions coding for, and methods for producing monovalent and multivalent single-chain antigen-binding molecules capable of glycosylation are described and claimed. Composition of, genetic constructions coding for, and methods for producing glycosylated monovalent and multivalent single-chain antigen-binding molecules capable of polyalkylene oxide conjugation are described and claimed. The invention also relates to methods for producing a polypeptide having increased glycosylation and the polypeptide produced by the described method. Uses resulting from the multifunctionality of a glycosylated/polyalkylene oxide conjugated antigen-binding protein are also described and claimed.

    摘要翻译： 本发明涉及能够进行糖基化的单链抗原结合分子。 描述和要求保护编码的组成，以及产生能够糖基化的单价和多价单链抗原结合分子的方法。 描述和要求保护编码的遗传结构的组成，以及能够产生聚环氧烷共轭的糖基化单价和多价单链抗原结合分子的方法。 本发明还涉及生产具有增加的糖基化的多肽的方法和通过所述方法产生的多肽。 还描述和要求保护由糖基化/聚环氧烷结合的抗原结合蛋白的多功能性产生的用途。

    Method for targeted delivery of nucleic acids
    7.
    发明授权
    Method for targeted delivery of nucleic acids 有权
    靶向递送核酸的方法

    公开(公告)号：US06333396B1

    公开(公告)日：2001-12-25

    申请号：US09420592

    申请日：1999-10-19

    申请人： David R. Filpula ,  Maoliang Wang ,  Marc D. Whitlow

    发明人： David R. Filpula ,  Maoliang Wang ,  Marc D. Whitlow

    IPC分类号： C12P2108

    CPC分类号： C07K16/3046 ,  C07K2317/31 ,  C07K2317/622 ,  C07K2319/00 ,  C12N15/87

    摘要： The present invention is directed to a method of in vivo and ex vivo gene delivery, for a variety of cells. More specifically, it relates to a novel carrier system and method for targeted delivery of nucleic acids to mammalian cells. More specifically, the present invention relates to carrier system comprising single-chain polypeptide binding molecules having an a region rich in basic amino acid and having the three dimensional folding and, thus, the binding ability and specificity, of the variable region of an antibody. The basic amino acid rich region can comprise oligo-lysine, oligo-arginine or combinations thereof. Such preparations of modified single chain polypeptide binding molecules also have ability to bind nucleic acids at the region rich in basic amino acid residues. These properties of the modified single chain polypeptide binding molecules make them very useful in a variety of therapeutic applications including gene therapy. The invention also relates to multivalent antigen-binding molecules having regions rich in basic amino acids. Compositions of, genetic constructions for, methods of use, and methods for producing basic amino acid tailed antigen-binding proteins are disclosed.

    摘要翻译： 本发明涉及各种细胞的体内和离体基因递送方法。 更具体地说，本发明涉及用于将核酸靶向递送至哺乳动物细胞的新的载体系统和方法。 更具体地，本发明涉及包含单链多肽结合分子的载体体系，其具有富含碱性氨基酸的区域，并且具有抗体可变区的三维折叠以及因此的结合能力和特异性。 碱性氨基酸富集区可以包含寡赖氨酸，寡精氨酸或其组合。 修饰的单链多肽结合分子的这种制剂还具有在富含碱性氨基酸残基的区域上结合核酸的能力。 修饰的单链多肽结合分子的这些性质使得它们在包括基因治疗在内的各种治疗应用中非常有用。 本发明还涉及具有富含碱性氨基酸的区域的多价抗原结合分子。 公开了用于制备碱性氨基酸尾抗原结合蛋白的遗传构建，使用方法和方法的组合物。

    Arginine deiminase derived from mycoplasma arthritidis and polymer conjugates containing the same
    8.
    发明授权
    Arginine deiminase derived from mycoplasma arthritidis and polymer conjugates containing the same 有权
    衍生自关节炎支原体的精氨酸脱亚胺酶和含有它的聚合物共轭物

    公开(公告)号：US6132713A

    公开(公告)日：2000-10-17

    申请号：US271713

    申请日：1999-03-18

    申请人： David Ray Fiipula ,  Maoliang Wang

    发明人： David Ray Fiipula ,  Maoliang Wang

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/46 ,  A61K38/50 ,  A61K47/48 ,  A61P35/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/78 ,  C12N11/08 ,  C12N11/10 ,  C12R1/35 ,  A61K38/54 ,  C12N9/14

    CPC分类号： A61K38/50 ,  C12N11/08 ,  C12N11/10 ,  C12N9/78

    摘要： A purified arginine deiminase (ADI) obtained from Mycoplasma arthritidis having the amino acid sequence of SEQ ID NO:2 as well as an isolated nucleic acid molecule containing a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:1 are disclosed. Other aspects of the invention include an expression vector, a cloned gene for expressing the Mycoplasma arthritidis derived ADI, (recombinant) host cells useful in expressing the ADI of the present invention and substantially non-antigenic polymer conjugates containing the ADI of the present invention as well as methods of treating arginine deiminase susceptible conditions in mammals. The arginine deiminase-polymer conjugates have high levels of retained enzyme activity and relatively long circulating lives.

    摘要翻译： 公开了从具有SEQ ID NO：2的氨基酸序列的关节炎支原体获得的纯化精氨酸脱亚氨酶（ADI）以及含有编码SEQ ID NO：1所示的氨基酸序列的核苷酸序列的分离的核酸分子。 本发明的其它方面包括表达载体，用于表达来源于关节炎支原体的ADI的克隆基因，用于表达本发明的ADI的重组宿主细胞和含有本发明的ADI的基本上非抗原性的聚合物缀合物，作为 以及在哺乳动物中治疗精氨酸脱亚胺酶易感条件的方法。 精氨酸脱亚氨酶 - 聚合物共轭物具有高水平的保留酶活性和较长的循环寿命。

    Arginine deiminase derived from Mycoplasma arthritidis and polymer conjugates containing the same
    9.
    发明授权
    Arginine deiminase derived from Mycoplasma arthritidis and polymer conjugates containing the same 失效
    衍生自关节炎支原体的精氨酸脱亚胺酶和含有它的聚合物共轭物

    公开(公告)号：US5916793A

    公开(公告)日：1999-06-29

    申请号：US105908

    申请日：1998-06-26

    申请人： David Ray Filpula ,  Maoliang Wang

    发明人： David Ray Filpula ,  Maoliang Wang

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/46 ,  A61K38/50 ,  A61K47/48 ,  A61P35/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/78 ,  C12N11/08 ,  C12N11/10 ,  C12R1/35 ,  C12N9/14 ,  C07H21/04

    CPC分类号： A61K38/50 ,  C12N11/08 ,  C12N11/10 ,  C12N9/78

    摘要： A purified arginine deiminase (ADI) obtained from Mycoplasma arthritidis having the amino acid sequence of SEQ ID NO:2 as well as an isolated nucleic acid molecule containing a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:1 are disclosed. Other aspects of the invention include an expression vector, a cloned gene for expressing the Mycoplasma arthritidis derived ADI, (recombinant) host cells useful in expressing the ADI of the present invention and substantially non-antigenic polymer conjugates containing the ADI of the present invention as well as methods of treating arginine deiminase susceptible conditions in mammals. The arginine deiminase-polymer conjugates have high levels of retained enzyme activity and relatively long circulating lives.

    摘要翻译： 公开了从具有SEQ ID NO：2的氨基酸序列的关节炎支原体获得的纯化精氨酸脱亚氨酶（ADI）以及含有编码SEQ ID NO：1所示的氨基酸序列的核苷酸序列的分离的核酸分子。 本发明的其它方面包括表达载体，用于表达来源于关节炎支原体的ADI的克隆基因，用于表达本发明的ADI的重组宿主细胞和含有本发明的ADI的基本上非抗原性的聚合物缀合物，作为 以及在哺乳动物中治疗精氨酸脱亚胺酶易感条件的方法。 精氨酸脱亚氨酶 - 聚合物共轭物具有高水平的保留酶活性和较长的循环寿命。