公开(公告)号：US20050161329A1

公开(公告)日：2005-07-28

申请号：US10511282

申请日：2003-04-14

申请人： Katariina Hutterer ,  Nels Olson ,  Stevan Jovanovich ,  Thomas Armstrong ,  Dean Burgi ,  Vladislav Dolnik

发明人： Katariina Hutterer ,  Nels Olson ,  Stevan Jovanovich ,  Thomas Armstrong ,  Dean Burgi ,  Vladislav Dolnik

IPC分类号： G01N21/64 ,  G01N27/447 ,  G01N27/453 ,  G01N30/46 ,  G02B21/00

CPC分类号： G01N27/44782 ,  G01N21/6486 ,  G01N27/44721 ,  G01N27/44743 ,  G01N27/44773 ,  G01N30/463 ,  G01N2021/6439 ,  G02B21/0028 ,  G02B21/004 ,  G02B21/0076

摘要： Multi-capillary systems for high-throughput electrophoretic separation and detection of biomolecules are disclosed. One embodiment of the invention uses galactomannans as a size-sieving matrix for multi-channel electrophoretic separations of biomolecules. Multi-color detection for the simultaneous analysis of controls and standards in the same channels as the samples, and endogenous fluorescence detection are also disclosed. Another embodiment of the invention is a two dimensional system for separation of complex samples, using multiplexed capillary electrophoresis system as the second dimension, with a fraction collection step connecting the two separation steps. The systems allow for separations to be accomplished with a highly parallel manner, or in a two-dimensional format.

摘要翻译： 公开了用于高通量电泳分离和生物分子检测的多毛细管系统。 本发明的一个实施方案使用半乳甘露聚糖作为尺寸筛选基质用于生物分子的多通道电泳分离。 还公开了用于同时分析与样品相同通道中的对照和标准物以及内源性荧光检测的多色检测。 本发明的另一个实施方案是使用多重毛细管电泳系统作为第二维度分离复杂样品的二维系统，连接两个分离步骤的级分收集步骤。 这些系统允许以高度平行的方式或以二维格式来实现分离。

公开(公告)号：US20070158193A1

公开(公告)日：2007-07-12

申请号：US11462990

申请日：2006-08-07

申请人： Dean Burgi ,  Stephen Williams

发明人： Dean Burgi ,  Stephen Williams

IPC分类号： B01D57/02 ,  G01N27/00

CPC分类号： G01N27/4473 ,  G01N27/44704 ,  G01N27/44717 ,  G01N27/44773

摘要： The present invention is directed to methods for identifying one or more analytes in a sample using electrophoresis. In one embodiment, the method comprises performing an electrophoretic separation by applying a potential across the separation path and thus generating a current and power therein and producing an electropherogram, integrating the current or the power to determine the cumulative current or power as a function of the separation time, transforming the electropherogram to a second electropherogram representing the signal as a function of the cumulative current or power, and identifying in the second electropherogram peaks that are correlated with the analytes in the sample. The invention also provides systems for performing the analysis and identification methods, as well as computer-readable products for performing the steps associated with the above methods.

摘要翻译： 本发明涉及使用电泳鉴定样品中一种或多种分析物的方法。 在一个实施例中，该方法包括通过在分离路径上施加电位并因此在其中产生电流和功率来产生电泳图来进行电泳分离，从而产生电泳图，积分电流或功率以确定累积电流或功率作为 分离时间，将电泳图转换成表示作为累积电流或功率的函数的信号的第二电泳图，并且在第二电泳图中鉴定与样品中的分析物相关的峰。 本发明还提供用于执行分析和识别方法的系统，以及用于执行与上述方法相关的步骤的计算机可读产品。

公开(公告)号：US20050115837A1

公开(公告)日：2005-06-02

申请号：US10726321

申请日：2003-12-01

申请人： Dean Burgi ,  Stephen Williams

发明人： Dean Burgi ,  Stephen Williams

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/4473 ,  G01N27/44704 ,  G01N27/44717 ,  G01N27/44773

摘要： The present invention is directed to methods for identifying one or more analytes in a sample using electrophoresis. In one embodiment, the method comprises performing an electrophoretic separation by applying a potential across the separation path and thus generating a current and power therein and producing an electropherogram, integrating the current or the power to determine the cumulative current or power as a function of the separation time, transforming the electropherogram to a second electropherogram representing the signal as a function of the cumulative current or power, and identifying in the second electropherogram peaks that are correlated with the analytes in the sample. The invention also provides systems for performing the analysis and identification methods, as well as computer-readable products for performing the steps associated with the above methods.

摘要翻译： 本发明涉及使用电泳鉴定样品中一种或多种分析物的方法。 在一个实施例中，该方法包括通过在分离路径上施加电位并因此在其中产生电流和功率来产生电泳图来进行电泳分离，从而产生电泳图，积分电流或功率以确定累积电流或功率作为 分离时间，将电泳图转换成表示作为累积电流或功率的函数的信号的第二电泳图，并且在第二电泳图中鉴定与样品中的分析物相关的峰。 本发明还提供用于执行分析和识别方法的系统，以及用于执行与上述方法相关的步骤的计算机可读产品。