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公开(公告)号：US08916503B2
公开(公告)日：2014-12-23
申请号：US10589052
申请日：2005-02-17
CPC分类号： C12Q1/6841 , C12Q2525/207 , C12Q2525/301 , C12Q2563/137 , C12Q2563/155 , C12Q2565/1015 , C12Q2565/102
摘要： Methods of isolating cells or generating cell lines comprising the step of exposing the cells to signaling probes that produce a signal upon hybridization to a target sequence, as well as methods of quantifying the level of expression of an RNA of interest, methods for identifying genetic recombinational events in living cells and methods of generating a transgenic animal using the isolated cells. Methods for isolating a plurality of cells encoding a plurality of different RNAs associated with a same nucleic acid tag sequence, comprising the step of exposing the cells to a same signaling probe that produces a detectable signal upon hybridization to the same nucleic acid tag sequence, are also provided. Signaling probes and protease probes that form stem-loop structures, three-arm junction structures, and dumbbell structures may be used in the above methods.
摘要翻译： 分离细胞或产生细胞系的方法包括将细胞暴露于与靶序列杂交时产生信号的信号传导探针的步骤，以及定量目的RNA表达水平的方法，用于鉴定遗传重组的方法 活细胞中的事件和使用分离的细胞产生转基因动物的方法。 用于分离编码与相同核酸标签序列相关联的多个不同RNA的多个细胞的方法包括将细胞暴露于与同一核酸标签序列杂交时产生可检测信号的相同信号探针的步骤是 也提供。 在上述方法中可以使用形成茎环结构，三臂连接结构和哑铃结构的信号探针和蛋白酶探针。
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2.发明申请METHODS AND COMPOSITIONS FOR IDENTIFYING AND VALIDATING MODULATORS OF CELL FATE 审中-公开鉴定和鉴定细胞因子的调节剂的方法和组合物公开(公告)号：US20120276572A1
公开(公告)日：2012-11-01
申请号：US13387962
申请日：2010-07-30
CPC分类号： C12Q1/6897 , C12N15/85 , C12Q1/6888 , C12Q2600/158 , G01N33/5061 , G01N33/5073 , C12Q2525/207
摘要： The invention provides for compositions and methods for identifying and validating modulators of cell fate, such as such as maintenance, cell specification, cell determination, induction of stem cell fate, cell differentiation, cell dedifferentiation, and cell trans-differentiation. The invention relates to reporter nucleic acid constructs, host cells comprising such constructs, and methods using such cells and constructs. The invention relates to methods for making cells comprising one or more reporter nucleic acid constructs using fluorogenic oligonucleotides. The methods relate to high throughput screens.
摘要翻译： 本发明提供用于鉴定和验证细胞命运调节剂的组合物和方法，例如维持，细胞规格，细胞测定，干细胞命运的诱导，细胞分化，细胞去分化和细胞转分化。 本发明涉及报道核酸构建体，包含这种构建体的宿主细胞，以及使用这种细胞和构建体的方法。 本发明涉及使用荧光寡核苷酸制备包含一种或多种报道核酸构建体的细胞的方法。 这些方法涉及高通量筛选。
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公开(公告)号：US20120058918A1
公开(公告)日：2012-03-08
申请号：US13147327
申请日：2010-02-01
CPC分类号： C12N9/14 , C12Y306/03049 , G01N33/502 , G01N33/5038 , G01N2333/914 , G01N2500/00 , G01N2800/382
摘要： Disclosed herein are cells and cell lines that stably express CFTR and methods for using those cells and cell lines. The invention also includes techniques for creating these cells and cell lines. The cells and cell lines of this invention are physiologically relevant. They are highly sensitive and provide consistent and reliable results in cell-based assays.
摘要翻译： 本文公开了稳定表达CFTR的细胞和细胞系以及使用那些细胞和细胞系的方法。 本发明还包括用于产生这些细胞和细胞系的技术。 本发明的细胞和细胞系在生理学上是相关的。 它们是高度敏感的，并在基于细胞的测定中提供一致和可靠的结果。
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4.发明授权Methods and compositions for identifying and validating modulators of cell fate 有权用于鉴定和验证细胞命运调节剂的方法和组合物公开(公告)号：US08945848B2
公开(公告)日：2015-02-03
申请号：US13387962
申请日：2010-07-30
CPC分类号： C12Q1/6897 , C12N15/85 , C12Q1/6888 , C12Q2600/158 , G01N33/5061 , G01N33/5073 , C12Q2525/207
摘要： The invention provides for compositions and methods for identifying and validating modulators of cell fate, such as such as maintenance, cell specification, cell determination, induction of stem cell fate, cell differentiation, cell dedifferentiation, and cell trans-differentiation. The invention relates to reporter nucleic acid constructs, host cells comprising such constructs, and methods using such cells and constructs. The invention relates to methods for making cells comprising one or more reporter nucleic acid constructs using fluorogenic oligonucleotides. The methods relate to high throughput screens.
摘要翻译： 本发明提供用于鉴定和验证细胞命运调节剂的组合物和方法，例如维持，细胞规格，细胞测定，干细胞命运的诱导，细胞分化，细胞去分化和细胞转分化。 本发明涉及报道核酸构建体，包含这种构建体的宿主细胞，以及使用这种细胞和构建体的方法。 本发明涉及使用荧光寡核苷酸制备包含一种或多种报道核酸构建体的细胞的方法。 这些方法涉及高通量筛选。
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公开(公告)号：US20090106853A1
公开(公告)日：2009-04-23
申请号：US10589052
申请日：2005-02-17
CPC分类号： C12Q1/6841 , C12Q2525/207 , C12Q2525/301 , C12Q2563/137 , C12Q2563/155 , C12Q2565/1015 , C12Q2565/102
摘要： The present invention relates to methods of isolating cells or generating cell lines using signaling probes that produce a signal upon hybridization to a target sequence. Other methods that utilize the signaling probe include methods of quantifying the level of RNA expression, methods for identifying genetic recombinational events in living cells and methods of generating a transgenic animal using the isolated cells. The invention also provides protease probes. Signaling probes and protease probes that form stem-loop structures, three-arm junction structures, and dumbbell structures are provided.
摘要翻译： 本发明涉及使用在与靶序列杂交时产生信号的信号探针来分离细胞或产生细胞系的方法。 使用信号探针的其它方法包括定量RNA表达水平的方法，用于鉴定活细胞中遗传重组事件的方法和使用分离的细胞产生转基因动物的方法。 本发明还提供蛋白酶探针。 提供形成茎环结构，三臂连接结构和哑铃结构的信号探针和蛋白酶探针。
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