公开(公告)号：US20090220430A1

公开(公告)日：2009-09-03

申请号：US12355777

申请日：2009-01-17

Applicant: Milind Rajopadhye ,  Kevin Groves ,  Dorin V. Preda

Inventor： Milind Rajopadhye ,  Kevin Groves ,  Dorin V. Preda

IPC: A61K49/00 ,  C07K5/06 ,  C07K5/08 ,  C07K5/10 ,  C07K5/12 ,  C07K7/06 ,  C07K7/08 ,  G01N33/53

CPC classification number: A61K49/0032 ,  A61K47/65 ,  A61K49/0056 ,  C07K5/1019 ,  C07K7/06 ,  C07K7/64 ,  C09B23/0008 ,  C09B23/083 ,  C09B23/086

Abstract: Provided is a family of intramolecularly quenched imaging agents for use in both in vivo and in vitro imaging that contain at least one enzymatically cleavable oligopeptide and two fluorophores or a fluorophore and a quencher. When subjected to proteolytic cleavage, at least one fluorophore is unquenched and becomes capable of producing a fluorescent signal upon excitation with light of an appropriate wavelength. Also provided are in vivo and in vitro imaging methods using such imaging agents.

Abstract translation: 提供了用于体内和体外成像的分子内骤冷成像剂家族，其包含至少一种可酶促切割的寡肽和两个荧光团或荧光团和猝灭剂。 当进行蛋白水解切割时，至少一个荧光团是不淬灭的，并且在用适当波长的光激发时变得能够产生荧光信号。 还提供了使用这种成像剂的体内和体外成像方法。

公开(公告)号：US08815214B2

公开(公告)日：2014-08-26

申请号：US12355777

申请日：2009-01-17

Applicant: Milind Rajopadhye ,  Kevin Groves ,  Dorin V. Preda

Inventor： Milind Rajopadhye ,  Kevin Groves ,  Dorin V. Preda

IPC: A61B5/00 ,  A61B8/00 ,  A61B10/00 ,  A61K38/12 ,  C07K5/00 ,  C07K7/00 ,  C07K16/00 ,  C07K17/00 ,  A61K38/00 ,  A61K38/04

CPC classification number: A61K49/0032 ,  A61K47/65 ,  A61K49/0056 ,  C07K5/1019 ,  C07K7/06 ,  C07K7/64 ,  C09B23/0008 ,  C09B23/083 ,  C09B23/086

Abstract: Provided is a family of intramolecularly quenched imaging agents for use in both in vivo and in vitro imaging that contain at least one enzymatically cleavable oligopeptide and two fluorophores or a fluorophore and a quencher. When subjected to proteolytic cleavage, at least one fluorophore is unquenched and becomes capable of producing a fluorescent signal upon excitation with light of an appropriate wavelength. Also provided are in vivo and in vitro imaging methods using such imaging agents.

Abstract translation: 提供了用于体内和体外成像的分子内骤冷成像剂家族，其包含至少一种可酶促切割的寡肽和两个荧光团或荧光团和猝灭剂。 当进行蛋白水解切割时，至少一个荧光团是不淬灭的，并且在用适当波长的光激发时变得能够产生荧光信号。 还提供了使用这种成像剂的体内和体外成像方法。