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公开(公告)号：US20240360284A1
公开(公告)日：2024-10-31
申请号：US18606320
申请日：2024-03-15
发明人： Jonas Gasparavicius , Vaidotas Kiseliovas , Karolis Leonavicius , Linas Mazutis , Juozas Nainys , Andrius Sinkunas , Giedrius Zolubas , Rapolas Zilionis , Vaida Kurmauskaite , Greta Ruksnaityte , Ignas Ragaisis , Duygucan Gul
IPC分类号： C08J3/12 , B01J13/16 , C08J3/075 , C12Q1/6806 , C12Q1/6816
CPC分类号： C08J3/126 , B01J13/16 , C08J3/075 , C08J2301/02 , C08J2305/02 , C12Q1/6806 , C12Q1/6816
摘要： Provided herein are core-shell microcapsules useful for compartmentalizing biological molecules in solution. Also provided are processes for manufacturing core-shell microcapsules and methods for using core-shell microcapsules to compartmentalize and optionally process biological entities and molecules.
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公开(公告)号：US11958947B2
公开(公告)日：2024-04-16
申请号：US18159317
申请日：2023-01-25
发明人： Jonas Gasparavicius , Vaidotas Kiseliovas , Karolis Leonavicius , Linas Mazutis , Juozas Nainys , Andrius Sinkunas , Giedrius Zolubas , Rapolas Zilionis , Vaida Kurmauskaite , Greta Ruksnaityte , Ignas Ragaisis , Duygucan Gul
IPC分类号： C08J3/075 , B01J13/16 , C08J3/12 , C12Q1/6806 , C12Q1/6816
CPC分类号： C08J3/126 , B01J13/16 , C08J3/075 , C08J2301/02 , C08J2305/02 , C12Q1/6806 , C12Q1/6816
摘要： Provided herein are core-shell microcapsules useful for compartmentalizing biological molecules in solution. Also provided are processes for manufacturing core-shell microcapsules and methods for using core-shell microcapsules to compartmentalize and optionally process biological entities and molecules.
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公开(公告)号：US20240085287A1
公开(公告)日：2024-03-14
申请号：US18514235
申请日：2023-11-20
IPC分类号： G01N1/40 , B01J13/04 , B01J13/20 , C12Q1/6806
CPC分类号： G01N1/4077 , B01J13/046 , B01J13/206 , C12Q1/6806
摘要： This invention relates to methods and systems for isolation of species in semi-permeable capsules and processing of encapsulated species through series of steps and/or reactions. To produce capsules, first aqueous two-phase system (ATPS) droplets are generated using microfluidics system. Then the hydrogel shell layer is hardened by inducing polymerization. As exemplified in this invention to achieve concentric ATPS droplet formation density-matched PEGDA and Dextran polymer solutions can be used. Once a capsule is formed, its composition can be changed by adding new reagents or replacing out old ones (e.g. by resuspending capsules in desired aqueous solution). The hydrogel shell of semi-permeable capsules can be dissolved at selected step during multi-step procedures to release the encapsulated species. This invention exemplifies isolation of individual cells within capsules and using the encapsulated cells for genotypic and phenotypic analysis. This invention also exemplifies use of capsules in multi-step procedures to perform complex biological reactions.
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公开(公告)号：US20240068928A1
公开(公告)日：2024-02-29
申请号：US18250601
申请日：2022-10-22
发明人： Karolis LEONAVICIUS , Dalius KUCIAUSKAS , Juozas NAINYS , Rytis KISIELIUS , Dangiras LUKOSIUS , Jonas GASPARAVICIUS
CPC分类号： G01N15/1459 , G01N15/0205 , G01N15/1463 , G01N21/6456
摘要： The instrument for processing microscopic particles may include a combination of modules integrated into a functional system. The system may be composed of synchronized subsystems including pneumatic modules, fluorometers, multi-wavelength lasers, dual view microscopy, high-voltage generators and other subsystems designed to analyze and control microfluidic processes occurring on a chip. The system performs the combined functions of microfluidic particle generation, analysis, and selection, making it possible for the user to create custom integrated workflows for molecular and cell biology processing. Creating the integrated platform requires an innovative and multi-disciplinary approach for module mechanical integration, electronic synchronization and user interface design for controlling diverse microfluidic processes at high speed.
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公开(公告)号：US20230340210A1
公开(公告)日：2023-10-26
申请号：US18159317
申请日：2023-01-25
发明人： Jonas GASPARAVICIUS , Vaidotas KISELIOVAS , Karolis LEONAVICIUS , Linas MAZUTIS , Juozas NAINYS , Andrius SINKUNAS , Giedrius ZOLUBAS , Rapolas ZILIONIS , Vaida KURMAUSKAITE , Greta RUKSNAITYTE , Ignas RAGAISIS , Duygucan GUL
CPC分类号： C08J3/126 , C08J3/075 , B01J13/16 , C08J2305/02 , C08J2301/02 , C12Q1/6806
摘要： Provided herein are core-shell microcapsules useful for compartmentalizing biological molecules in solution. Also provided are processes for manufacturing core-shell microcapsules and methods for using core-shell microcapsules to compartmentalize and optionally process biological entities and molecules.
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公开(公告)号：US11860076B2
公开(公告)日：2024-01-02
申请号：US16934045
申请日：2020-07-21
IPC分类号： G01N1/40 , C12Q1/68 , B01J13/04 , B01J13/20 , C12Q1/6806
CPC分类号： G01N1/4077 , B01J13/046 , B01J13/206 , C12Q1/6806
摘要： The present invention relates to methods and systems for isolation of species in semi-permeable capsules and processing of encapsulated species through series of steps and/or reactions. To produce capsules, first aqueous two-phase system (ATPS) droplets are generated using microfluidics system and then the hydrogel shell layer is hardened by inducing polymerization. As exemplified in this invention to achieve concentric ATPS droplet formation density-matched PEGDA and Dextran polymer solutions can be used. Once a capsule is formed, its composition can be changed by adding new reagents or replacing out old ones (e.g. by resuspending capsules in desired aqueous solution). The hydrogel shell of semi-permeable capsules can be dissolved at selected step during multi-step procedures in order to release the encapsulated species. The present invention exemplifies the isolation of individual cells within capsules and using the encapsulated cells for genotypic and phenotypic analysis. Finally, the present invention also exemplifies the use of capsules in multi-step procedures to perform complex biological reactions.
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