公开(公告)号：US20120315624A1

公开(公告)日：2012-12-13

申请号：US13489689

申请日：2012-06-06

申请人： Eddie L. Chang ,  Kelly L. Robertson

发明人： Eddie L. Chang ,  Kelly L. Robertson

IPC分类号： G01N21/64

CPC分类号： C12Q1/70 ,  C12Q2565/626

摘要： As described herein, locked nucleic acids are used with flow cytometric-fluorescence in situ hybridization (LNA flow-FISH) detection of viral RNA in infected cells. This technique represents a straightforward way to monitor viral infection in cells and can be used to measure efficacy of potential antiviral compounds.

摘要翻译： 如本文所述，使用锁定的核酸与感染细胞中的病毒RNA的流式细胞术 - 荧光原位杂交（LNA flow-FISH）检测。 这种技术代表了一种直观的监测细胞病毒感染的方法，可用于测量潜在的抗病毒化合物的功效。

公开(公告)号：US07655474B2

公开(公告)日：2010-02-02

申请号：US11278502

申请日：2006-04-03

申请人： Sulay Jhaveri ,  Mario Ancona ,  Edward E Foos ,  Eddie L Chang

发明人： Sulay Jhaveri ,  Mario Ancona ,  Edward E Foos ,  Eddie L Chang

IPC分类号： G01N21/75

CPC分类号： B22F1/0018 ,  A61K47/6923 ,  A61K47/6929 ,  B01J2219/0063 ,  B01J2219/00637 ,  B22F1/0062 ,  B82Y15/00 ,  B82Y30/00

摘要： A method of making a nanoclusters functionalized with a single DNA strand comprising the steps of providing nanoclusters, combining said nanoclusters with thiolated DNA, incubating said nanoclusters and thiolated DNA mixture, combining said mixture with a solution comprising ethanol and dichloromethane; separating said mixture into an aqueous phase and an organic phase, mixing said aqueous phase with a solution comprising dicholormethane and NaCl, and separating the mixture into an aqueous phase and an organic phase; wherein said organic phase comprises said nanoclusters functionalized with a single DNA strand. Further, provided is a nanocluster functionalized with a single DNA strand comprising a nanocluster, said nanocluster being functionalized with a single DNA strand, said DNA strand having a length of about 10 to about 50 bases.

摘要翻译： 制备用单个DNA链功能化的纳米团簇的方法，包括以下步骤：提供纳米团簇，将所述纳米团簇与硫醇化DNA组合，孵育所述纳米团簇和硫醇化DNA混合物，将所述混合物与包含乙醇和二氯甲烷的溶液混合; 将所述混合物分离成水相和有机相，将所述水相与包含二氯甲烷和NaCl的溶液混合，并将混合物分离成水相和有机相; 其中所述有机相包含用单个DNA链官能化的所述纳米团簇。 此外，提供了用包含纳米簇的单个DNA链功能化的纳米团簇，所述纳米簇用单个DNA链进行官能化，所述DNA链具有约10至约50个碱基的长度。

公开(公告)号：US06297191B1

公开(公告)日：2001-10-02

申请号：US09532860

申请日：2000-03-22

申请人： Dhananjay Puranik ,  Robert E. Morris ,  Eddie L. Chang

发明人： Dhananjay Puranik ,  Robert E. Morris ,  Eddie L. Chang

IPC分类号： B01S2022

CPC分类号： C10G29/20

摘要： A composition including a solid substrate, a metal chelant, and a saturated hydrocarbon linker attached between the substrate and the chelant, wherein the chelant is an organic wherein the chelant is an organic macrocycle or polyol.

摘要翻译： 包含固体底物，金属螯合剂和连接在底物和螯合剂之间的饱和烃连接体的组合物，其中所述螯合剂是其中所述螯合剂是有机大环化合物或多元醇的有机物。

公开(公告)号：US06248842B1

公开(公告)日：2001-06-19

申请号：US09267920

申请日：1999-02-25

申请人： Alok Singh ,  Eddie L. Chang

发明人： Alok Singh ,  Eddie L. Chang

IPC分类号： C08F832

CPC分类号： C08F8/30

摘要： Selective, crosslinked chelating polymers are produced by substituting an acyclic chelating agent with a polymerizable functional group. The resulting substituted acyclic chelating agent is then complexed with the target metal ion. A crosslinkable monomer is then added and the complexed material is crosslinked. The complexed metal is then removed, providing a crosslinked polymeric chelating agent that has been templated for the target metal ion.

摘要翻译： 通过用可聚合官能团取代无环螯合剂制备选择性交联的螯合聚合物。 然后将所得的取代的无环螯合剂与目标金属离子络合。 然后加入可交联的单体，并将络合的材料交联。 然后去除络合的金属，提供已经被模板化为目标金属离子的交联聚合物螯合剂。

公开(公告)号：US20110027388A1

公开(公告)日：2011-02-03

申请号：US12847342

申请日：2010-07-30

申请人： Eddie L. Chang ,  Lisa Hensley ,  Dzung C. Thach ,  Andy Knight ,  Gene Olinger

发明人： Eddie L. Chang ,  Lisa Hensley ,  Dzung C. Thach ,  Andy Knight ,  Gene Olinger

IPC分类号： A61K33/24 ,  A61P31/18 ,  A61P31/14 ,  A61P31/12 ,  A61J1/05 ,  A61M5/00

CPC分类号： A61K31/185 ,  A61K9/0019 ,  A61K31/28 ,  A61K33/02 ,  A61K33/24 ,  A61K2300/00

摘要： Hexaamminecobalt(III) chloride, also called Cohex, reduces the extent of viral infection, including difficult to treat infections caused by Ebola virus and HIV. Disclosed are methods for treating a viral infection, comprising administering to a patient a cobalt(III) hexammine compound in an amount effective to reduce an extent of a viral infection. Also disclosed are kits for delivery of a cobalt(III) hexammine compound by injection.

摘要翻译： 也称为Cohex的六氰胺钴（III）（III）也减少了病毒感染的程度，包括难以治疗埃博拉病毒和艾滋病毒引起的感染。 公开了用于治疗病毒感染的方法，包括向患者施用有效减少病毒感染程度的钴（III）六胺化合物。 还公开了用于通过注射递送钴（III）六胺化合物的试剂盒。

公开(公告)号：US20100004187A1

公开(公告)日：2010-01-07

申请号：US12494750

申请日：2009-06-30

申请人： Eddie L. Chang ,  Daniel Zabetakis ,  Andy Knight ,  Monique L. van Hoek

发明人： Eddie L. Chang ,  Daniel Zabetakis ,  Andy Knight ,  Monique L. van Hoek

IPC分类号： A61K31/295 ,  A61K31/65 ,  A61K31/7048 ,  A61P31/04

CPC分类号： A61K31/295 ,  A61K31/65 ,  A61K31/7048 ,  Y02A50/473

摘要： Disclosed herein is a method of: administering to a subject suspected of being infected with a bacterium, a composition of a cobalt (III) compound having the formula CoR1R2R3R4R5R6 or a salt thereof and an antibiotic compound. Each of R1, R2, R3, R4, and R5 is the same or different and includes an N-based ligand donor atom selected from the group consisting of ammonia, primary amine, or secondary amine, or salt thereof. R6 is a ligand. Also disclosed herein is a composition of the above cobalt (III) compound and an antibiotic compound. Also disclosed herein is a method of administering the above cobalt (III) compound to a subject diagnosed as needing a broad spectrum antibiotic

摘要翻译： 本文公开了一种方法：向疑似感染细菌的受试者施用具有式CoR 1 R 3 R 4 R 5 R 6的钴（III）化合物或其盐和抗生素化合物的组合物。 R 1，R 2，R 3，R 4和R 5各自相同或不同，并且包括选自氨，伯胺或仲胺或其盐的基于N的配体供体原子。 R6是配体。 本文还公开了上述钴（III）化合物和抗生素化合物的组合物。 本文还公开了将上述钴（III）化合物施用于被诊断为需要广谱抗生素的受试者的方法

公开(公告)号：US20080182835A1

公开(公告)日：2008-07-31

申请号：US11934424

申请日：2007-11-02

申请人： Eddie L. Chang ,  James B. Delehanty ,  Dzung C. Thach ,  Jason Bongard ,  Andy Knight

发明人： Eddie L. Chang ,  James B. Delehanty ,  Dzung C. Thach ,  Jason Bongard ,  Andy Knight

IPC分类号： A61K31/555 ,  C12N5/06 ,  C07H21/04 ,  A61P31/12

CPC分类号： A61K31/555

摘要： The present invention is generally directed to a method of prophylaxis against viral infection of a cell or subject or a method of treating a subject infected with a virus including administering an antiviral composition having the general Structure III, wherein each of R1, R2, R3, R4, R5 and R6 is the same or different and includes an N-based ligand donor atom selected from the group consisting of ammonia, primary amine or secondary amine, or salt thereof. The present invention is also generally directed to a method of preparing an antiviral agent including providing a cobalt pentammine salt having a non-amine coordination site and mono-substituting the non-amine coordination site with a functional group incorporating a strong coordinator atom to cobalt to form a CoHex structure of Structure III, in which R1 incorporates the functional group having the strong coordinator atom coordinated with the cobalt atom, or a salt thereof.

摘要翻译： 本发明一般涉及预防病毒感染细胞或受试者的方法或治疗感染病毒的受试者的方法，包括给予具有通用结构III的抗病毒组合物，其中R 1， R 3，R 3，R 3，R 4，R 5和R 6， / SUB>相同或不同，并且包括选自氨，伯胺或仲胺或其盐的基于N的配体供体原子。 本发明还通常涉及一种制备抗病毒剂的方法，其包括提供具有非胺配位位点的钴五胺盐，并将非胺配位位点单一取代与钴结合的强配位体原子的官能团与钴 形成结构III的CoHex结构，其中R 1含有与钴原子配位的强配位体原子的官能团或其盐。

公开(公告)号：US6143849A

公开(公告)日：2000-11-07

申请号：US757172

申请日：1996-11-27

申请人： Dhananjay B. Puranik ,  Eddie L. Chang

发明人： Dhananjay B. Puranik ,  Eddie L. Chang

IPC分类号： C08F120/34 ,  C08F126/06 ,  C08F26/06

CPC分类号： C08F120/34 ,  C08F126/06

摘要： Multifunctional chelating groups are covalently bound to acrylate or methylate monomers. These monomers are polymerized. The resulting polymers are highly substituted with chelating groups and are useful for removing metals from fluids such as liquid hydrocarbons. An example of a polymer according to this invention has the following structure: ##STR1## wherein Q is a saturated or unsaturated aliphatic group having 1 to about 200 carbon atoms and is typically an unsubstituted unbranched alkylene, a substituted unbranched alkylene, a substituted branched alkylene, or an unsubstituted branched alkylene, and is an integer having a value of at least 1.

摘要翻译： 多功能螯合基团与丙烯酸酯或甲基丙烯酸酯单体共价结合。 这些单体聚合。 所得聚合物被螯合基团高度取代，并且可用于从诸如液体烃的流体中除去金属。 根据本发明的聚合物的实例具有以下结构：其中Q是具有1至约200个碳原子的饱和或不饱和脂族基团，并且通常为未取代的未分支的亚烷基，取代的未分支的亚烷基，取代的支链亚烷基，或 未取代的支链亚烷基，+ Z为至少为1的整数。

公开(公告)号：US6077421A

公开(公告)日：2000-06-20

申请号：US687115

申请日：1996-07-18

申请人： Dhananjay Puranik ,  Robert E. Morris ,  Eddie L. Chang

发明人： Dhananjay Puranik ,  Robert E. Morris ,  Eddie L. Chang

IPC分类号： C10G29/20 ,  C10G17/00

CPC分类号： C10G29/20

摘要： A composition including a solid substrate, a metal chelant, and a saturated hydrocarbon linker attached between the substrate and the chelant. The position is used in the method for removing from a liquid medium a metal having atomic number equal to or greater than 27, the method including the steps of passing the liquid medium through the composition that is in solid, particulate form and separating the medium from the composition.

摘要翻译： 包含固体底物，金属螯合剂和连接在底物和螯合剂之间的饱和烃连接物的组合物。 该组合物用于从液体介质中除去原子数大于或等于27的金属的方法，该方法包括以下步骤：使液体介质通过组合物，其为固体，颗粒形式并将介质从 组成。

公开(公告)号：US07932096B2

公开(公告)日：2011-04-26

申请号：US12574401

申请日：2009-10-06

申请人： Sulay Jhaveri ,  Mario Ancona ,  Edward E Foos ,  Eddie L Chang

发明人： Sulay Jhaveri ,  Mario Ancona ,  Edward E Foos ,  Eddie L Chang

IPC分类号： G01N21/75

CPC分类号： B22F1/0018 ,  A61K47/6923 ,  A61K47/6929 ,  B01J2219/0063 ,  B01J2219/00637 ,  B22F1/0062 ,  B82Y15/00 ,  B82Y30/00

摘要： A method of making a nanoclusters functionalized with a single DNA strand comprising the steps of providing nanoclusters, combining said nanoclusters with thiolated DNA, incubating said nanoclusters and thiolated DNA mixture, combining said mixture with a solution comprising ethanol and dichloromethane; separating said mixture into an aqueous phase and an organic phase, mixing said aqueous phase with a solution comprising dicholormethane and NaCl, and separating the mixture into an aqueous phase and an organic phase; wherein said organic phase comprises said nanoclusters functionalized with a single DNA strand. Further, provided is a nanocluster functionalized with a single DNA strand comprising a nanocluster, said nanocluster being functionalized with a single DNA strand, said DNA strand having a length of about 10 to about 50 bases.

摘要翻译： 制备用单个DNA链功能化的纳米团簇的方法，包括以下步骤：提供纳米团簇，将所述纳米团簇与硫醇化DNA组合，孵育所述纳米团簇和硫醇化DNA混合物，将所述混合物与包含乙醇和二氯甲烷的溶液混合; 将所述混合物分离成水相和有机相，将所述水相与包含二氯甲烷和NaCl的溶液混合，并将混合物分离成水相和有机相; 其中所述有机相包含用单个DNA链官能化的所述纳米团簇。 此外，提供了用包含纳米簇的单个DNA链功能化的纳米团簇，所述纳米簇用单个DNA链进行官能化，所述DNA链具有约10至约50个碱基的长度。