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    Method of producing sialylated oligosaccharides
    1.
    发明授权
    Method of producing sialylated oligosaccharides 有权
    生产唾液酸化寡糖的方法

    公开(公告)号：US08507227B2

    公开(公告)日：2013-08-13

    申请号：US12282125

    申请日：2007-03-07

    申请人： Eric Samain

    发明人： Eric Samain

    IPC分类号： C12P19/18

    CPC分类号： C12P19/18 ,  C12P19/26

    摘要： The present invention relates to a method for the large scale in vivo synthesis of sialylated oligosaccharides, culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as lactose, wherein said microorganism comprises heterologous genes encoding a CMP-Neu5Ac synthetase, a sialic acid synthase, a GlcNAc-6-phosphate 2 epimerase and a sialyltransferase, and wherein the endogenous genes coding for sialic acid aldolase (NanA) and for ManNac kinase (NanK) have been deleted or inactivated. The invention also relates to these micoorganisms which are capable of producing internally activated sialic acid.

    摘要翻译： 本发明涉及唾液酸化寡糖的大规模体内合成方法，在培养基中培养微生物，任选地包含外源前体如乳糖，其中所述微生物包含编码CMP-Neu5Ac合成酶的异源基因，唾液酸 酸合成酶，GlcNAc-6-磷酸二差向异构酶和唾液酸转移酶，其中编码唾液酸醛缩酶（NanA）和ManNac激酶（NanK）的内源基因已被缺失或失活。 本发明还涉及能够产生内部活化的唾液酸的这些微生物。

    HIGH YIELD PRODUCTION OF SIALIC ACID (NEU5AC) BY FERMENTATION
    3.
    发明申请
    HIGH YIELD PRODUCTION OF SIALIC ACID (NEU5AC) BY FERMENTATION 审中-公开
    通过发酵高效酸生产（NEU5AC）

    公开(公告)号：US20110165626A1

    公开(公告)日：2011-07-07

    申请号：US12444081

    申请日：2007-10-02

    申请人： Eric Samain

    发明人： Eric Samain

    IPC分类号： C12P19/28 ,  C12N1/00

    CPC分类号： C12P19/26 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52

    摘要： The present invention relates to a method for producing sialic acid, comprising the step of culturing a microorganism in a culture medium, wherein said microorganism comprises heterologous genes encoding a sialic acid synthase (NeuB), a UDP-GlcNAc epimerase (NeuC), said micro-organism being devoid of a gene encoding CMP-Neu5Ac synthase (NeuA) or wherein a gene encoding CMP-Neu5Ac synt hase (NeuA) has been inactivated or deleted; and wherein endogenous genes coding for sialic acid aldolase (NanA), for ManNac kinase (NanK) and for sialic acid transporter (NanT) have been deleted or inactivated. It also relates to the above microorganism.

    摘要翻译： 本发明涉及生产唾液酸的方法，其包括在培养基中培养微生物的步骤，其中所述微生物包括编码唾液酸合酶（NeuB），UDP-GlcNAc差向异构酶（NeuC）的异源基因，所述微生物 - 缺少编码CMP-Neu5Ac合成酶（NeuA）的基因的组织或其中编码CMP-Neu5Ac合成酶（NeuA）的基因已被灭活或缺失; 并且其中编码唾液酸醛缩酶（NanA），ManNac激酶（NanK）和唾液酸转运蛋白（NanT）的内源基因已被缺失或失活。 它也涉及上述微生物。

    METHOD OF PRODUCING SIALYLATED OLIGOSACCHARIDES
    4.
    发明申请
    METHOD OF PRODUCING SIALYLATED OLIGOSACCHARIDES 有权
    生产SIALYLATED OLIGOSACCHARIDES的方法

    公开(公告)号：US20110014661A1

    公开(公告)日：2011-01-20

    申请号：US12282125

    申请日：2007-03-07

    申请人： Eric Samain

    发明人： Eric Samain

    IPC分类号： C12P19/18 ,  C12N1/21 ,  C12N1/20

    CPC分类号： C12P19/18 ,  C12P19/26

    摘要： The present invention relates to a method for the large scale in vivo synthesis of sialylated oligosaccharides, culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as lactose, wherein said microorganism comprises heterologous genes encoding a CMP-Neu5Ac synthetase, a sialic acid synthase, a GlcNAc-6-phosphate 2 epimerase and a sialyltransferase, and wherein the endogenous genes coding for sialic acid aldolase (NanA) and for ManNac kinase (NanK) have been deleted or inactivated. The invention also relates to these micoorganisms which are capable of producing internally activated sialic acid.

    摘要翻译： 本发明涉及唾液酸化寡糖的大规模体内合成方法，在培养基中培养微生物，任选地包含外源前体如乳糖，其中所述微生物包含编码CMP-Neu5Ac合成酶的异源基因，唾液酸 酸合成酶，GlcNAc-6-磷酸二差向异构酶和唾液酸转移酶，其中编码唾液酸醛缩酶（NanA）和ManNac激酶（NanK）的内源基因已被缺失或失活。 本发明还涉及能够产生内部活化的唾液酸的这些微生物。

    Production of globosides oligosaccharides using metabolically engineered microorganisms
    6.
    发明申请
    Production of globosides oligosaccharides using metabolically engineered microorganisms 审中-公开
    使用代谢工程微生物生产球状寡糖

    公开(公告)号：US20070202578A1

    公开(公告)日：2007-08-30

    申请号：US11509818

    申请日：2006-08-25

    申请人： Eric Samain ,  Mialy Randriantsoa ,  Sophie Drouillard

    发明人： Eric Samain ,  Mialy Randriantsoa ,  Sophie Drouillard

    IPC分类号： C12P19/04 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/1048 ,  C12N9/90 ,  C12P19/18

    摘要： The present invention relates to the large scale in vivo synthesis of globosides oligosaccharides; especially globotriose, globotetraose and globopentaose, which are the carbohydrate portions of globotriosylceramide (Gb3Cer), globotetraosylceramide (Gb4Cer) and globopentaosylceramide (Gb5Cer) respectively. It also relates to high yield production of potential anticancer vaccines of the globo-series glycosphingolipids, including the Globo-H. It also relates to the use of the glycosyltransferase encoded by the lgtD gene from Haemophilus influenzae as a β1,3 galactosyl transferase to catalyze the transfer of a galactose moiety from UDP-Gal to an acceptor bearing the terminal non reducing structure GalNAcβ-3-R to form the Galβ-3GalNAcβ-3-R structure

    摘要翻译： 本发明涉及球蛋白寡糖的大规模体内合成; 特别是球三糖，球四糖和球蛋白五糖，它们分别是球三糖基神经酰胺（Gb 3 Cer），球形四糖基神经酰胺（Gb4Cer）和globopentaosylceramide（Gb5Cer）的碳水化合物部分。 它还涉及包括Globo-H在内的Globo系列鞘糖脂的潜在抗癌疫苗的高产量生产。 它还涉及由流感嗜血杆菌的lgtD基因编码的糖基转移酶用作β1,3半乳糖基转移酶以催化半乳糖部分从UDP-Gal转移到携带末端非还原结构GalNAcβ3-R的受体 形成Galbeta-3GalNAcβ3-R结构

    Method for producing oligopolysaccharides
    7.
    发明授权
    Method for producing oligopolysaccharides 有权
    寡聚多糖的制备方法

    公开(公告)号：US08586332B2

    公开(公告)日：2013-11-19

    申请号：US11930663

    申请日：2007-10-31

    申请人： Eric Samain ,  Bernard Priem

    发明人： Eric Samain ,  Bernard Priem

    IPC分类号： C12P19/26 ,  C12P19/18 ,  C12N9/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C07H3/06 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26

    摘要： The invention concerns the production by microbiological process of oligopolysaccharides of biological interest. More particularly, the invention concerns a method for synthesizing in vivo the oligopolysaccharides by internalization of an exogenous precursor in growing bacterial cells expressing adequate modifying and glycosylating genes.

    摘要翻译： 本发明涉及通过微生物学方法生产具有生物学兴趣的寡聚多糖。 更具体地说，本发明涉及通过在表达足够的修饰和糖基化基因的生长细菌细胞中外源化外源前体体内合成寡聚多糖的方法。

    PRODUCTION OF GLOBOSIDES OLIGOSACCHARIEDS USING METABOLICALLY ENGINEERED MICROORGANISMS
    8.
    发明申请
    PRODUCTION OF GLOBOSIDES OLIGOSACCHARIEDS USING METABOLICALLY ENGINEERED MICROORGANISMS 审中-公开
    使用代谢工程化微生物生产全身寡聚寡糖

    公开(公告)号：US20110236934A1

    公开(公告)日：2011-09-29

    申请号：US12955553

    申请日：2010-11-29

    申请人： Eric Samain ,  Mialy Randriantsoa ,  Sophie Drouillard ,  Tatiana Pierret

    发明人： Eric Samain ,  Mialy Randriantsoa ,  Sophie Drouillard ,  Tatiana Pierret

    IPC分类号： C12P19/18 ,  C12N1/00 ,  C07H3/06 ,  C07H3/00 ,  A23L1/30

    CPC分类号： C12N9/1048 ,  C12N9/90 ,  C12P19/18

    摘要： The present invention relates to the large scale in vivo synthesis of globosides oligosaccharides; especially globotriose, globotetraose and globopentaose, which are the carbohydrate portions of globotriosylceramide (Gb3Cer), globotetraosylceramide (Gb4Cer) and globopentaosylceramide (Gb5Cer) respectively. It also relates to high yield production of potential anticancer vaccines of the globo-series glycosphingo lipids, including the Globo-H. It also relates to the use of the glycosyltransferase encoded by the lgtD gene from Haemophilus influenzae as a β1,3 galactosyl transferase to catalyze the transfer of a galactose moiety from UDP-Gal to an acceptor bearing the terminal non reducing structure GalNAcβ-3-R to form the Galβ-3GalNAcβ-3-R structure

    摘要翻译： 本发明涉及球蛋白寡糖的大规模体内合成; 特别是球三糖，球四糖和球蛋白五糖，它们分别是球三糖基神经酰胺（Gb 3 Cer），球形四糖基神经酰胺（Gb4Cer）和globopentaosylceramide（Gb5Cer）的碳水化合物部分。 它还涉及包括Globo-H在内的Globo系列鞘糖脂类潜在抗癌疫苗的高产量生产。 它还涉及使用由流感嗜血杆菌的lgtD基因编码的糖基转移酶作为一种1,3-半乳糖基转移酶来催化半乳糖部分从UDP-Gal转移到具有末端非还原结构的受体GalNAc和bgr- 3-R形成Gal和bgr; -3GalNAc和bgr-3-R结构