公开(公告)号：US08673822B2

公开(公告)日：2014-03-18

申请号：US10586045

申请日：2004-12-30

申请人： Jung-Hoon Sohn ,  Eui-Sung Choi ,  Jung-Hoon Bae ,  Eung-Suck Lee ,  Mi-Kyung Shin

发明人： Jung-Hoon Sohn ,  Eui-Sung Choi ,  Jung-Hoon Bae ,  Eung-Suck Lee ,  Mi-Kyung Shin

IPC分类号： C40B20/04 ,  C40B30/06 ,  C40B50/06

CPC分类号： C12P21/02 ,  C07K14/535 ,  C07K14/55 ,  C07K2319/00 ,  C07K2319/35 ,  C07K2319/61 ,  C12N9/20 ,  C12N15/1086

摘要： Disclosed are a method for rapid screening suitable translational fusion partners (TFPs) capable of inducing expression or secretory production of non-producible proteins, which are difficult to produce in conventional recombinant production methods, from a variety of genetic sources, and protein secretion-inducing TFPs obtained using the method.

摘要翻译： 公开了一种快速筛选能够诱导来自多种遗传来源的常规重组生产方法难以产生的非生产性蛋白质的表达或分泌生产的合适的翻译融合伴侣（TFP）的方法，以及蛋白质分泌诱导 使用该方法获得的TFP。

公开(公告)号：US20100196350A1

公开(公告)日：2010-08-05

申请号：US12438354

申请日：2007-08-23

申请人： Hyo Jeong Hong ,  Jung-Whoi Lee ,  Jin Man Kim ,  Yeon Sung Son ,  Eung Suck Lee

发明人： Hyo Jeong Hong ,  Jung-Whoi Lee ,  Jin Man Kim ,  Yeon Sung Son ,  Eung Suck Lee

IPC分类号： A61K39/395 ,  A61K31/7088 ,  A61P35/00 ,  G01N33/53

CPC分类号： C07K16/2803 ,  A61K2039/505 ,  C07K14/70503 ,  C07K2317/73 ,  C07K2317/76 ,  C12N15/1138 ,  C12N2310/14

摘要： Disclosed herein are a pharmaceutical composition for inhibiting the growth or metastasis of cholangiocarcinoiria, comprising a L1CAM activity inhibitor or expression suppressor and a treatment method using the composition. This is based on the finding that L1CAM is overexpressed on cholangiocarcinoma and plays an important role in the growth and metastasis of cholangiocarcinoma and the mortality of cholangiocarcinoma patients increases as the expression rate of L1CAM increases. Also, antibodies inhibitory of the activity of L1CAM, or siRNAs suppressing the expression of L1CAM, are found to reduce the growth and invasion of cholangiocarcinoma cells. Mouse monoclonal antibodies, recognizing the L1CAM protein on the cholangiocarcinoma cell surface and binding specifically to cholangiocarcinoma tissues, or siRNAs, antisense oligonucleotides or shRNAs, may be useful in the treatment of cholangiocarcinoma by inhibiting the growth, invasion and migration of cholangiocarcinoma cell.

摘要翻译： 本文公开了一种用于抑制胆管癌细胞生长或转移的药物组合物，其包含L1CAM活性抑制剂或表达抑制剂以及使用该组合物的治疗方法。 这是基于L1CAM在胆管癌上过表达的发现，并且在胆管癌的生长和转移中起重要作用，胆管癌患者的死亡率随着L1CAM的表达增加而升高。 此外，发现抑制L1CAM的活性的抗体或抑制L1CAM的表达的siRNA减少胆管癌细胞的生长和侵袭。 识别胆管癌细胞表面上的L1CAM蛋白并特异性结合胆管癌组织或siRNA，反义寡核苷酸或shRNA的小鼠单克隆抗体可用于通过抑制胆管癌细胞的生长，侵袭和迁移来治疗胆管癌。

公开(公告)号：US08153122B2

公开(公告)日：2012-04-10

申请号：US12438354

申请日：2007-08-23

申请人： Hyo Jeong Hong ,  Jung-Whoi Lee ,  Jin Man Kim ,  Yeon Sung Son ,  Eung Suck Lee

发明人： Hyo Jeong Hong ,  Jung-Whoi Lee ,  Jin Man Kim ,  Yeon Sung Son ,  Eung Suck Lee

IPC分类号： A61K39/395

CPC分类号： C07K16/2803 ,  A61K2039/505 ,  C07K14/70503 ,  C07K2317/73 ,  C07K2317/76 ,  C12N15/1138 ,  C12N2310/14

摘要： Disclosed herein are a pharmaceutical composition for inhibiting the growth or metastasis of cholangiocarcinoma, comprising a L1CAM activity inhibitor or expression suppressor and a treatment method using the composition. This is based on the finding that L1CAM is overexpressed on cholangiocarcinoma and plays an important role in the growth and metastasis of cholangiocarcinoma and the mortality of cholangiocarcinoma patients increases as the expression rate of L1CAM increases. Also, antibodies inhibitory of the activity of L1CAM, or siRNAs suppressing the expression of L1CAM, are found to reduce the growth and invasion of cholangiocarcinoma cells. Mouse monoclonal antibodies, recognizing the L1CAM protein on the cholangiocarcinoma cell surface and binding specifically to cholangiocarcinoma tissues, or siRNAs, antisense oligonucleotides or shRNAs, may be useful in the treatment of cholangiocarcinoma by inhibiting the growth, invasion and migration of cholangiocarcinoma cell.

摘要翻译： 本文公开了一种用于抑制胆管癌生长或转移的药物组合物，其包含L1CAM活性抑制剂或表达抑制剂以及使用该组合物的治疗方法。 这是基于L1CAM在胆管癌上过表达的发现，并且在胆管癌的生长和转移中起重要作用，胆管癌患者的死亡率随着L1CAM的表达增加而升高。 此外，发现抑制L1CAM的活性的抗体或抑制L1CAM的表达的siRNA减少胆管癌细胞的生长和侵袭。 识别胆管癌细胞表面上的L1CAM蛋白并特异性结合胆管癌组织或siRNA，反义寡核苷酸或shRNA的小鼠单克隆抗体可用于通过抑制胆管癌细胞的生长，侵袭和迁移来治疗胆管癌。

公开(公告)号：US20070275385A1

公开(公告)日：2007-11-29

申请号：US10586045

申请日：2004-12-30

申请人： Jung-Hoon Sohn ,  Eui-Sung Choi ,  Jung-Hoon Bae ,  Eung-Suck Lee ,  Mi-Kyung Shin

发明人： Jung-Hoon Sohn ,  Eui-Sung Choi ,  Jung-Hoon Bae ,  Eung-Suck Lee ,  Mi-Kyung Shin

IPC分类号： C12P21/04 ,  C07H21/04 ,  C07K14/00 ,  C12Q1/68 ,  C12N15/00 ,  C12N5/00

CPC分类号： C12P21/02 ,  C07K14/535 ,  C07K14/55 ,  C07K2319/00 ,  C07K2319/35 ,  C07K2319/61 ,  C12N9/20 ,  C12N15/1086

摘要： Disclosed are a method for rapid screening suitable translational fusion partners (TFPs) capable of inducing expression or secretory production of non-producible proteins, which are difficult to produce in conventional recombinant production methods, from a variety of genetic sources, and protein secretion-inducing TFPs obtained using the method.

摘要翻译： 公开了一种快速筛选能够诱导来自多种遗传来源的常规重组生产方法难以产生的非生产性蛋白质的表达或分泌生产的合适的翻译融合伴侣（TFP）的方法，以及蛋白质分泌诱导 使用该方法获得的TFP。