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公开(公告)号：US20160319306A1
公开(公告)日：2016-11-03
申请号：US15191657
申请日：2016-06-24
申请人： Laurence GIRBAL , Florence Saint-Prix , Mathieu Cesari , Henri-Pierre Fierobe , Roberto Mazzoli , Florence Mingardon , Angélique Chanal-Vial
发明人： Laurence GIRBAL , Florence Saint-Prix , Mathieu Cesari , Henri-Pierre Fierobe , Roberto Mazzoli , Florence Mingardon , Angélique Chanal-Vial
摘要： The present invention relates, inter alia, to recombinant Gram-positive Clostridia host cells for producing solvents, fuels and/or chemical intermediates, and preferably ethanol, from plant cell walls comprising: (a) at least one nucleic acid encoding a plant cell wall degrading enzyme, wherein the host cells produce and secrete the plant cell wall degrading enzyme, (b) at least one nucleic acid encoding an enzyme that converts pyruvate to acetaldehyde and at least one nucleic acid encoding an enzyme that converts acetaldehyde to ethanol wherein the host cell is capable of expressing said nucleic acid, and, (c) a mutation in at least one nucleic acid encoding for an enzyme in a metabolic pathway which produces a metabolite other than acetaldehyde from pyruvate or ethanol from acetaldehyde, such that the mutation results in a reduced production of the metabolite.
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公开(公告)号：US20110129876A1
公开(公告)日：2011-06-02
申请号：US13056352
申请日：2009-07-30
CPC分类号： C12N9/2437 , C07K2319/00 , C07K2319/20 , C07K2319/70 , C12P21/02 , Y02E50/16 , Y02P20/52
摘要： Described herein are molecules, constructs and methods for the production and secretion of polypeptides of interest by host cells, preferably bacterial host cells, and more particularly gram positive bacteria. In particular, the present invention is related to a polynucleic acid encoding a fusion protein and to uses thereof for the secretion of heterologous or homologous polypeptides of interest by a bacterial host cell, preferably Clostridium bacteria. The present invention further relates to methods and constructs for the production and secretion of heterologous or homologous polypeptides of interest proteins by host cells using such polynucleic acids and fusion proteins.
摘要翻译： 本文描述了用于由宿主细胞，优选细菌宿主细胞，更特别是革兰氏阳性细菌产生和分泌目的多肽的分子，构建体和方法。 特别地，本发明涉及编码融合蛋白的多核酸及其用于细菌宿主细胞（优选梭菌属细菌）分泌异源或同源多肽的用途。 本发明还涉及使用这种多核酸和融合蛋白的宿主细胞产生和分泌异源或同源多肽的方法和构建体。
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公开(公告)号：US08735133B2
公开(公告)日：2014-05-27
申请号：US13056352
申请日：2009-07-30
CPC分类号： C12N9/2437 , C07K2319/00 , C07K2319/20 , C07K2319/70 , C12P21/02 , Y02E50/16 , Y02P20/52
摘要： Described herein are molecules, constructs and methods for the production and secretion of polypeptides of interest by host cells, preferably bacterial host cells, and more particularly gram positive bacteria. In particular, the present invention is related to a polynucleic acid encoding a fusion protein and to uses thereof for the secretion of heterologous or homologous polypeptides of interest by a bacterial host cell, preferably Clostridium bacteria. The present invention further relates to methods and constructs for the production and secretion of heterologous or homologous polypeptides of interest proteins by host cells using such polynucleic acids and fusion proteins.
摘要翻译： 本文描述了用于由宿主细胞，优选细菌宿主细胞，更特别是革兰氏阳性细菌产生和分泌目的多肽的分子，构建体和方法。 特别地，本发明涉及编码融合蛋白的多核酸及其用于细菌宿主细胞（优选梭菌属细菌）分泌异源或同源多肽的用途。 本发明还涉及使用这种多核酸和融合蛋白的宿主细胞产生和分泌异源或同源多肽的方法和构建体。
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4.发明申请公开(公告)号：US20130115669A1
公开(公告)日：2013-05-09
申请号：US13737600
申请日：2013-01-09
申请人： Laurence GIRBAL , Florence Saint-Prix , Mathieu Cesari , Henri-Pierre Fierobe , Roberto Mazzoli , Florence Mingardon , Angélique Chanal-Vial
发明人： Laurence GIRBAL , Florence Saint-Prix , Mathieu Cesari , Henri-Pierre Fierobe , Roberto Mazzoli , Florence Mingardon , Angélique Chanal-Vial
摘要： The present invention relates, inter alia, to recombinant Gram-positive Clostridia host cells for producing solvents, fuels and/or chemical intermediates, and preferably ethanol, from plant cell walls comprising: (a) at least one nucleic acid encoding a plant cell wall degrading enzyme, wherein the host cells produce and secrete the plant cell wall degrading enzyme, (b) at least one nucleic acid encoding an enzyme that converts pyruvate to acetaldehyde and at least one nucleic acid encoding an enzyme that converts acetaldehyde to ethanol wherein the host cell is capable of expressing said nucleic acid, and, (c) a mutation in at least one nucleic acid encoding for an enzyme in a metabolic pathway which produces a metabolite other than acetaldehyde from pyruvate or ethanol from acetaldehyde, such that the mutation results in a reduced production of the metabolite.
摘要翻译： 本发明特别涉及用于从植物细胞壁生产溶剂，燃料和/或化学中间体，优选乙醇的重组革兰氏阳性梭菌宿主细胞，其包含：（a）编码植物细胞壁的至少一种核酸 降解酶，其中宿主细胞产生和分泌植物细胞壁降解酶，（b）至少一种编码将丙酮酸转化为乙醛的酶的核酸和至少一种编码将乙醛转化为乙醇的酶的核酸，其中所述宿主 细胞能够表达所述核酸，和（c）编码代谢途径中的酶的至少一种核酸中的突变，所述代谢途径从乙醛从丙酮酸或乙醇产生不同于乙醛的代谢物，使得突变导致 减少代谢产物的产生。
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5.发明申请公开(公告)号：US20110236943A1
公开(公告)日：2011-09-29
申请号：US13132227
申请日：2009-12-02
申请人： Laurence Girbal , Florence Saint-Prix , Mathieu Cesari , Henri-Pierre Fierobe , Roberto Mazzoli , Florence Mingardon , Angélique Chanal-Vial
发明人： Laurence Girbal , Florence Saint-Prix , Mathieu Cesari , Henri-Pierre Fierobe , Roberto Mazzoli , Florence Mingardon , Angélique Chanal-Vial
摘要： The present invention relates, inter alia, to recombinant Gram-positive Clostridia host cells for producing solvents, fuels and/or chemical intermediates, and preferably ethanol, from plant cell walls comprising: (a) at least one nucleic acid encoding a plant cell wall degrading enzyme, wherein the host cells produce and secrete the plant cell wall degrading enzyme, (b) at least one nucleic acid encoding an enzyme that converts pyruvate to acetaldehyde and at least one nucleic acid encoding an enzyme that converts acetaldehyde to ethanol wherein the host cell is capable of expressing said nucleic acid, and, (c) a mutation in at least one nucleic acid encoding for an enzyme in a metabolic pathway which produces a metabolite other than acetaldehyde from pyruvate or ethanol from acetaldehyde, such that the mutation results in a reduced production of the metabolite.
摘要翻译： 本发明特别涉及用于从植物细胞壁生产溶剂，燃料和/或化学中间体，优选乙醇的重组革兰氏阳性梭菌宿主细胞，其包含：（a）编码植物细胞壁的至少一种核酸 降解酶，其中宿主细胞产生和分泌植物细胞壁降解酶，（b）至少一种编码将丙酮酸转化为乙醛的酶的核酸和至少一种编码将乙醛转化为乙醇的酶的核酸，其中所述宿主 细胞能够表达所述核酸，和（c）编码代谢途径中的酶的至少一种核酸中的突变，所述代谢途径从乙醛从丙酮酸或乙醇产生不同于乙醛的代谢物，使得突变导致 减少代谢产物的产生。
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