公开(公告)号：US4288553A

公开(公告)日：1981-09-08

申请号：US120590

申请日：1980-02-11

申请人： Prithipal Singh ,  Pyare L. Khanna ,  Floyd W. Colvin

发明人： Prithipal Singh ,  Pyare L. Khanna ,  Floyd W. Colvin

IPC分类号： C07D213/56 ,  C12N9/96 ,  G01N33/535 ,  G01N33/54

CPC分类号： C07D213/56 ,  C12N9/96 ,  G01N33/535 ,  Y10S435/964 ,  Y10S436/811 ,  Y10S436/819 ,  Y10S530/806

摘要： Novel carboxyalkylated disopyramides (NORPACE.RTM.) are provided as precursors for conjugating proteins, either antigenic for the preparation of antibodies or enzymatic for the preparation of enzyme conjugates, which antibodies and enzyme conjugates find use as reagents in immunoassays. The combination of antibodies and enzyme conjugates provide for sensitive, accurate, rapid assays for disopyramide without interference from closely analogous compounds, such as methadone or disopyramides metabolites.

摘要翻译： 提供新的羧基烷基化二吡咯酰胺（NORPACE TM）作为用于缀合蛋白质的前体，其用于制备抗体的抗原或用于制备酶缀合物的酶，所述抗体和酶缀合物用作免疫测定中的试剂。 抗体和酶偶联物的组合提供对吡吡胺的敏感，准确，快速的测定，而不受密切类似的化合物如美沙酮或二吡啶酰胺代谢物的干扰。

公开(公告)号：US4404278A

公开(公告)日：1983-09-13

申请号：US209365

申请日：1980-11-24

申请人： Mae W. Hu ,  Floyd W. Colvin ,  Edwin F. Ullman

发明人： Mae W. Hu ,  Floyd W. Colvin ,  Edwin F. Ullman

IPC分类号： G01N33/53 ,  C07K16/40 ,  G01N33/531 ,  G01N33/54 ,  C12N9/96

CPC分类号： C07K16/40 ,  G01N33/531 ,  Y10S435/81 ,  Y10S435/968 ,  Y10S436/808

摘要： Methods and compositions for assaying for coenzymes having N-substituted 1,4-dihydropyridyl as the active portion of the prosthetic group. The conversion of NAD to NADH is widely measured both for determining NAD-NADH (including the analogs thereof) dependent enzymes and enzymes that can be coupled to NAD-NADH dependent enzymes, and for determining ligands or receptors in competitive protein binding assays. Antibodies specific for NADH in the presence of NAD are employed, providing enhanced fluorescence over the normal fluorescence of unbound NADH. By determining the rate of formation of NADH or the concentration of NADH, the analyte or enzyme of interest can be determined.

摘要翻译： 用于测定具有N-取代的1,4-二氢吡啶作为假体基团的活性部分的辅酶的方法和组合物。 广泛测量NAD对NADH（包括其类似物）依赖性酶和可与NAD-NADH依赖性酶偶联的酶以及用于测定竞争性蛋白质结合测定中的配体或受体的NAD-NADH（NADP）的转化。 使用在NAD存在下对NADH有特异性的抗体，与未结合的NADH的正常荧光相比提供增强的荧光。 通过测定NADH的形成速率或NADH的浓度，可以确定所关注的分析物或酶。

公开(公告)号：US4518701A

公开(公告)日：1985-05-21

申请号：US500503

申请日：1983-06-02

申请人： Pyare L. Khanna ,  Floyd W. Colvin

发明人： Pyare L. Khanna ,  Floyd W. Colvin

IPC分类号： G01N33/536 ,  G01N33/543 ,  G01N33/52

CPC分类号： G01N33/536 ,  G01N33/54393 ,  Y10S436/80 ,  Y10S436/825

摘要： Methods are provided for reducing non-specific interference in competitive protein binding assays employing as the labeled reagent a fluorescent conjugate of a hydrophobic ligand conjugated to a fluorescer, which in turn is bound to a water soluble polysaccharide carrier ("fluorescer conjugate reagent"). In order to reduce non-specific interference from physiologic samples, the fluorescent reagent is combined with a lipid substituted neutral support, under conditions which provides two binding fractions: a first weakly binding fraction which is relatively free of non-specific interference in a competitive protein binding assay employing physiological fluids; and a second fraction, which more strongly binds to the lipid substituted support.

摘要翻译： 提供了用于降低竞争性蛋白质结合测定中的非特异性干扰的方法，其使用与荧光剂缀合的疏水性配体的荧光缀合物作为标记试剂，其结合水溶性多糖载体（“荧光剂缀合试剂”）。 为了减少生理样品的非特异性干扰，在提供两种结合级分的条件下，将荧光试剂与脂质取代的中性支持物组合：在竞争性蛋白质中相对没有非特异性干扰的第一弱结合级分 使用生理流体的结合测定; 和与脂质取代的支持物更强烈结合的第二级分。