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17 条记录 (耗时 0.019 秒)

    Methods and compositions for assaying for the production of 1,4-dihydropyridyl
    1.
    发明授权
    Methods and compositions for assaying for the production of 1,4-dihydropyridyl 失效
    用于测定1,4-二氢吡啶的生产的方法和组合物

    公开(公告)号：US4404278A

    公开(公告)日：1983-09-13

    申请号：US209365

    申请日：1980-11-24

    申请人： Mae W. Hu ,  Floyd W. Colvin ,  Edwin F. Ullman

    发明人： Mae W. Hu ,  Floyd W. Colvin ,  Edwin F. Ullman

    IPC分类号： G01N33/53 ,  C07K16/40 ,  G01N33/531 ,  G01N33/54 ,  C12N9/96

    CPC分类号： C07K16/40 ,  G01N33/531 ,  Y10S435/81 ,  Y10S435/968 ,  Y10S436/808

    摘要： Methods and compositions for assaying for coenzymes having N-substituted 1,4-dihydropyridyl as the active portion of the prosthetic group. The conversion of NAD to NADH is widely measured both for determining NAD-NADH (including the analogs thereof) dependent enzymes and enzymes that can be coupled to NAD-NADH dependent enzymes, and for determining ligands or receptors in competitive protein binding assays. Antibodies specific for NADH in the presence of NAD are employed, providing enhanced fluorescence over the normal fluorescence of unbound NADH. By determining the rate of formation of NADH or the concentration of NADH, the analyte or enzyme of interest can be determined.

    摘要翻译： 用于测定具有N-取代的1,4-二氢吡啶作为假体基团的活性部分的辅酶的方法和组合物。 广泛测量NAD对NADH（包括其类似物）依赖性酶和可与NAD-NADH依赖性酶偶联的酶以及用于测定竞争性蛋白质结合测定中的配体或受体的NAD-NADH（NADP）的转化。 使用在NAD存在下对NADH有特异性的抗体，与未结合的NADH的正常荧光相比提供增强的荧光。 通过测定NADH的形成速率或NADH的浓度，可以确定所关注的分析物或酶。

    Geminal diphenyl derivatives and their use in immunoassays
    2.
    发明授权
    Geminal diphenyl derivatives and their use in immunoassays 失效
    Geminal二苯衍生物及其在免疫测定中的应用

    公开(公告)号：US5256575A

    公开(公告)日：1993-10-26

    申请号：US755203

    申请日：1991-09-04

    申请人： Mae W. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

    发明人： Mae W. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

    IPC分类号： C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  C07C309/15 ,  G01N33/53 ,  G01N33/78 ,  G01N33/94 ,  G01N33/566

    CPC分类号： C07C309/15 ,  C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  G01N33/5306 ,  G01N33/78 ,  G01N33/94 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/825

    摘要： A kit for inactivating interfering binding proteins in a immunoassay for a member of a specific binding pair (sbp). The method comprises including in an assay medium containing a sample suspected of containing an sbp member and an interfering binding protein an effective amount of a water soluble compound having two substituted or unsubstituted phenyl groups linked to a common atom. When the sbp member or its sbp partner has two phenyl groups linked to a common atom, the compound has a number of-groups other than hydrogen attached to the phenyl groups and the atom that differs by at least two from the number of such groups on the sbp member. When the sbp member or its sbp partner has two phenyl groups linked to a common atom and the binding protein is not an antibody, the compound has only one group other than hydrogen attached to a phenyl group or the common atom. The methods have particular application in avoiding-cross-reactivity of non-analyte materials in a sample with immunachemical reagents used in such assay. The methods-have application also in disrupting complexer between an analyte to be determined and other materials to that one can accurately determine the amount of an analyte in a sample.

    摘要翻译： 用于在特异性结合对（sbp）的成员的免疫测定中灭活干扰结合蛋白的试剂盒。 该方法包括在含有疑似含有sbp成员和干扰结合蛋白的样品的测定培养基中包含有效量的具有两个与常见原子连接的取代或未取代的苯基的水溶性化合物。 当sbp成员或其sbp伴侣具有连接到共同原子的两个苯基时，该化合物具有与苯基连接的氢以外的多个基团，并且与该基团的数目不同的至少两个的原子在 sbp成员。 当sbp成员或其sbp伴侣具有连接到共同原子的两个苯基并且结合蛋白不是抗体时，该化合物除了连接到苯基或共同原子上的氢以外仅具有一个基团。 该方法特别适用于避免样品中非分析物质与这种测定中使用的免疫化学试剂的交叉反应。 所述方法还应用于破坏要测定的待分析物与其他材料之间的复合物，以使得可以准确地确定样品中分析物的量。

    Geminal diphenyl derivatives and their use in immunoassays
    4.
    发明授权
    Geminal diphenyl derivatives and their use in immunoassays 失效
    GEMINAL DIPHENYL衍生物及其在免疫中的应用

    公开(公告)号：US5063165A

    公开(公告)日：1991-11-05

    申请号：US289998

    申请日：1988-12-22

    申请人： Mae W. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

    发明人： Mae W. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

    IPC分类号： C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  C07C309/15 ,  G01N33/53 ,  G01N33/78 ,  G01N33/94

    CPC分类号： G01N33/94 ,  C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  C07C309/15 ,  G01N33/5306 ,  G01N33/78 ,  Y10S436/815 ,  Y10S436/825 ,  Y10S436/826

    摘要： Methods are disclosed for inactivating interfering binding proteins in a immunoassay for a member of a specific binding pair (sbp). The method comprises including in an assay medium containing a sample suspected of containing an sbp member and an interfering binding protein an effective amount of a water soluble compound having two substituted or unsubstituted phenyl groups linked to a common atom. When the sbp member or its sbp partner has two phenyl groups linked to a common atom, the compound has a number of groups other than hydrogen attached to the phenyl groups and the atom that differs by at least two from the number of such groups on the sbp member. When the sbp member or its sbp partner has two phenyl groups linked to a common atom and the binding protein is not an antibody, the compound has only one group other than hydrogen attached to a phenyl group or the common atom. The methods have particular application in avoiding cross-reactivity of non-analyte materials in a sample with immunochemical reagents used in such assay. The methods have application also in disrupting complexes between an analyte to be determined and other materials so that one can accurately determine the amount of an analyte in a sample.

    Nortriptyline conjugates to antigenic proteins and enzymes
    5.
    发明授权
    Nortriptyline conjugates to antigenic proteins and enzymes 失效
    Nortriptyline与抗原蛋白和酶结合

    公开(公告)号：US6008411A

    公开(公告)日：1999-12-28

    申请号：US480633

    申请日：1995-06-07

    申请人： Mae W. Hu ,  Prithipal Singh

    发明人： Mae W. Hu ,  Prithipal Singh

    IPC分类号： G01N33/531 ,  G01N33/94 ,  C07C233/00

    CPC分类号： G01N33/531 ,  G01N33/9466

    摘要： Nortriptyline functionalized compounds are provided for conjugation to antigenic compositions, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies, which find use in immunoassays for the determination of nortriptyline in a sample, while the enzyme conjugates find use in an enzyme assay for the determination of nortriptyline in a sample.

    摘要翻译： 提供了Nortriptyline官能化化合物用于与抗原组合物，特别是聚（氨基酸）和酶缀合。 抗体结合物用于生产抗体，其用于免疫测定用于测定样品中的去甲替林，而酶缀合物可用于测定样品中去甲替林的酶测定。

    Theophylline antigens and antibodies
    6.
    发明授权
    Theophylline antigens and antibodies 失效
    茶碱抗原和抗体

    公开(公告)号：US4230805A

    公开(公告)日：1980-10-28

    申请号：US2527

    申请日：1979-01-11

    申请人： Prithipal Singh ,  Mae W. Hu

    发明人： Prithipal Singh ,  Mae W. Hu

    IPC分类号： C07D473/04 ,  G01N33/94 ,  C07G7/00 ,  C07G7/02

    CPC分类号： C07D473/04 ,  G01N33/94 ,  Y10S435/961 ,  Y10S435/964 ,  Y10S530/806

    摘要： Theophylline derivatives are provided for preparing reagents for use in competitive protein binding assays and for use as reagents in competitive protein binding assays. Theophylline is substituted at the 3 position and conjugated to antigens for production of antibodies which specifically recognize theophylline as distinct from structurally similar analogs such as caffeine. Enzyme conjugates are provided which find use for measuring the amount of theophylline in a sample suspected of containing theophylline. A method is provided employing the reagents for the determination of theophylline.

    摘要翻译： 提供茶碱衍生物用于制备用于竞争性蛋白质结合测定的试剂，并用作竞争性蛋白质结合测定中的试剂。 茶碱在3位被取代并与抗原结合，用于产生特异性识别茶碱与结构上类似的类似物如咖啡因不同的抗体。 提供了酶缀合物，其用于测量怀疑含有茶碱的样品中茶碱的量。 提供了用于测定茶碱的试剂的方法。

    Amitriptyline conjugates to antigenic proteins and enzymes
    9.
    发明授权
    Amitriptyline conjugates to antigenic proteins and enzymes 失效
    阿米替林与抗原蛋白和酶结合

    公开(公告)号：US4307245A

    公开(公告)日：1981-12-22

    申请号：US137658

    申请日：1980-04-07

    申请人： Mae W. Hu ,  Prithipal Singh

    发明人： Mae W. Hu ,  Prithipal Singh

    IPC分类号： C07K16/44 ,  G01N33/531 ,  C07C87/459 ,  C07C101/28 ,  C07C101/34

    CPC分类号： G01N33/531 ,  C07K16/44 ,  Y10S436/823

    摘要： Amitriptyline functionalized compounds are provided for conjugation to antigenic compositions, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies, which find particular use in immunoassays for the determination of amitriptyline, while the enzyme conjugate finds use in a homogeneous enzyme immunoassay for the determination of amitriptyline.

    摘要翻译： 提供阿米替林官能化化合物用于与抗原组合物，特别是聚（氨基酸）和酶缀合。 抗体结合物用于产生抗体，其在免疫测定中特别用于测定阿米替林，而酶缀合物可用于均相酶免疫测定中用于测定阿米替林。

    Theophylline antigens and antibodies
    10.
    发明授权
    Theophylline antigens and antibodies 失效
    茶碱抗原和抗体

    公开(公告)号：US4156081A

    公开(公告)日：1979-05-22

    申请号：US787829

    申请日：1977-04-15

    申请人： Prithipal Singh ,  Mae W. Hu

    发明人： Prithipal Singh ,  Mae W. Hu

    IPC分类号： C07D473/06 ,  C07D473/04 ,  C07K14/00 ,  C07K16/00 ,  C12N9/00 ,  C12N9/06 ,  C12N9/14 ,  C12N9/24 ,  G01N33/53 ,  G01N33/94

    CPC分类号： C07D473/04 ,  G01N33/94 ,  Y10S435/961 ,  Y10S435/964 ,  Y10S530/807

    摘要： Theophylline derivatives are provided for preparing reagents for use in competitive protein binding assays and for use as reagents in competitive protein binding assays. Theophylline is substituted at the 3 position and conjugated to antigens for production of antibodies which specifically recognize theophylline as distinct from structurally similar analogs such as caffeine. Enzyme conjugates are provided which find use for measuring the amount of theophylline in a sample suspected of containing theophylline. A method is provided employing the reagents for the determination of theophylline.

    摘要翻译： 提供茶碱衍生物用于制备用于竞争性蛋白质结合测定的试剂，并用作竞争性蛋白质结合测定中的试剂。 茶碱在3位被取代并与抗原结合，用于产生特异性识别茶碱与结构上类似的类似物如咖啡因不同的抗体。 提供了酶缀合物，其用于测量怀疑含有茶碱的样品中茶碱的量。 提供了用于测定茶碱的试剂的方法。