High speed microscope with spectral resolution
    1.
    发明授权
    High speed microscope with spectral resolution 有权
    具有光谱分辨率的高速显微镜

    公开(公告)号:US09103721B2

    公开(公告)日:2015-08-11

    申请号:US13439951

    申请日:2012-04-05

    摘要: A system and method of high-speed microscopy using a two-photon microscope with spectral resolution. The microscope is operable to provide two- to five-dimensional fluorescence images of samples, including two or three spatial dimensions, a spectral dimension (for fluorescence emission), and a temporal dimension (on a scale of less than approximately one second). Two-dimensional (spatial) images with a complete wavelength spectrum are generated from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The line scans may be formed using one or more of curved mirrors and lenses. The multiple beams may be formed using one of a grating, an array of lenses, and a beam splitter.

    摘要翻译: 使用具有光谱分辨率的双光子显微镜的高速显微镜的系统和方法。 显微镜可操作以提供样品的二维至五维荧光图像,包括两个或三个空间维度,光谱维度(用于荧光发射)和时间维度(小于约一秒的刻度)。 从样品的单次扫描产生具有完整波长谱的二维(空间)图像。 显微镜可以包括多光束点扫描显微镜,单束线扫描显微镜和多光束线扫描显微镜中的一个。 线扫描可以使用一个或多个弯曲镜和透镜来形成。 多个光束可以使用光栅,透镜阵列和分束器中的一个来形成。

    HIGH SPEED MICROSCOPE WITH SPECTRAL RESOLUTION
    4.
    发明申请
    HIGH SPEED MICROSCOPE WITH SPECTRAL RESOLUTION 有权
    具有光谱分辨率的高速显微镜

    公开(公告)号:US20120257196A1

    公开(公告)日:2012-10-11

    申请号:US13439951

    申请日:2012-04-05

    IPC分类号: G01J3/00

    摘要: A system and method of high-speed microscopy using a two-photon microscope with spectral resolution. The microscope is operable to provide two- to five-dimensional fluorescence images of samples, including two or three spatial dimensions, a spectral dimension (for fluorescence emission), and a temporal dimension (on a scale of less than approximately one second). Two-dimensional (spatial) images with a complete wavelength spectrum are generated from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The line scans may be formed using one or more of curved mirrors and lenses. The multiple beams may be formed using one of a grating, an array of lenses, and a beam splitter.

    摘要翻译: 使用具有光谱分辨率的双光子显微镜的高速显微镜的系统和方法。 显微镜可操作以提供样品的二维至五维荧光图像,包括两个或三个空间维度,光谱维度(用于荧光发射)和时间维度(小于约一秒的刻度)。 从样品的单次扫描产生具有完整波长谱的二维(空间)图像。 显微镜可以包括多光束点扫描显微镜,单束线扫描显微镜和多光束线扫描显微镜中的一个。 线扫描可以使用一个或多个弯曲镜和透镜来形成。 多个光束可以使用光栅,透镜阵列和分束器中的一个来形成。