公开(公告)号：US07498133B2

公开(公告)日：2009-03-03

申请号：US10676872

申请日：2003-09-30

申请人： Galina Fomovskaia ,  Martin Alexander Smith ,  James Critser Davis ,  Kevin Jones ,  Mikhail A. Fomovsky

发明人： Galina Fomovskaia ,  Martin Alexander Smith ,  James Critser Davis ,  Kevin Jones ,  Mikhail A. Fomovsky

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B01J20/28033 ,  B01J20/28023 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3248 ,  C12N1/06 ,  C12N15/1017 ,  G01N21/64 ,  G01N33/5308

摘要： There is provided a substrate for lysing cells and purifying nucleic acid having a matrix and a coating and an integrity maintainer for maintaining the purified nucleic acid. Also provided is a method of purifying nucleic acid by applying a nucleic acid sample to a substrate having an anionic detergent affixed to a matrix, the substrate physically capturing the nucleic acid, bonding the nucleic acid to a substrate and generating a signal when the nucleic acid bonds to the substrate indicating the presence of the nucleic acid. A kit for purifying nucleic acid containing a coated matrix and an integrity maintenance provider for preserving the matrix and purifying nucleic acid is also provided.

摘要翻译： 提供了用于裂解细胞并纯化具有基质和用于保持纯化的核酸的包衣和完整性维持剂的核酸的底物。 还提供了通过将核酸样品施加到具有粘附到基质上的阴离子洗涤剂的底物，底物物理捕获核酸，将核酸结合到底物并在核酸上产生信号时，产生信号的方法 键合到底物，表明存在核酸。 还提供了用于纯化含有包被基质的核酸的试剂盒和用于保存基质和纯化核酸的完整性维持提供者。

公开(公告)号：US06746841B1

公开(公告)日：2004-06-08

申请号：US09736659

申请日：2000-12-14

申请人： Galina Fomovskaia ,  Martin Alexander Smith ,  James Critser Davis ,  Kevin Jones ,  Mikhail A. Fomovsky

发明人： Galina Fomovskaia ,  Martin Alexander Smith ,  James Critser Davis ,  Kevin Jones ,  Mikhail A. Fomovsky

IPC分类号： C12Q168

CPC分类号： B01J20/28033 ,  B01J20/28023 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3248 ,  C12N1/06 ,  C12N15/1017 ,  G01N21/64 ,  G01N33/5308

摘要： There is provided a substrate for lysing calls and purifying nucleic acid having a matrix and a coating and an integrity maintainer for maintaining the purified nucleic acid. Also provided is a method of purifying nucleic acid by applying a nucleic acid sample to a substrate having an anionic detergent affixed to a matrix, the substrate physically capturing the nucleic acid, bonding the nucleic acid to a substrate and generating a signal when the nucleic acid bonds to the substrate indicating the presence of the nucleic acid. A kit for purifying nucleic acid containing a coated matrix and an integrity maintenance provider for preserving the matrix and purifying nucleic acid is also provided.

摘要翻译： 提供了用于裂解呼叫并纯化具有基质和用于保持纯化的核酸的包衣和完整性维持剂的核酸的底物。 还提供了通过将核酸样品施加到具有粘附到基质上的阴离子洗涤剂的底物，底物物理捕获核酸，将核酸结合到底物并在核酸上产生信号时，产生信号的方法 键合到底物，表明存在核酸。 还提供了用于纯化含有包被基质的核酸的试剂盒和用于保存基质和纯化核酸的完整性维持提供者。

公开(公告)号：US20060094015A1

公开(公告)日：2006-05-04

申请号：US10530146

申请日：2003-10-03

申请人： Martin Smith ,  Galina Fomovskaia ,  Mikhail Fomovsky

发明人： Martin Smith ,  Galina Fomovskaia ,  Mikhail Fomovsky

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N1/08

CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12N15/1017 ,  C12Q2523/308

摘要： The present invention relates to methods for isolating and storing, nucleic acid from a sample containing nucleic acid, such as a cell sample or cell lysate. The nucleic acid is isolated on a solid phase medium, which is then dried, and which can be stored efficiently, such as at room temperature, in columns, tubes, and microwell plates having a wide variety of filters and other solid phase media, for extended periods of time, including days, weeks, and months. The invention provides methods for isolating and storing nucleic acid from a sample by applying the sample to a solid phase medium, retaining the cells, lysing the cellular retentate, drying the medium and retaining the nucleic acid, storing the nucleic acid for extended periods of time at room temperature and humidity, and optionally eluting the nucleic acid. The invention provides methods for storing nucleic acid-containing samples on a wide range of solid phase media in many types of tubes, columns, or multiwell plates, many of which are commercial available.

摘要翻译： 本发明涉及从含有核酸例如细胞样品或细胞裂解液的样品中分离和储存核酸的方法。 核酸在固相培养基上分离，然后将其干燥，并且可以有效地存储，例如室温下，具有各种过滤器和其它固相培养基的柱，管和微孔板中，用于 延长的时间，包括天，周和月。 本发明提供了通过将样品应用于固相培养基，保留细胞，裂解细胞滞留物，干燥培养基和保留核酸，将核酸延长时间来分离和储存来自样品的核酸的方法 在室温和湿度下，并任选地洗脱核酸。 本发明提供了在许多类型的管，柱或多孔板中在宽范围的固相培养基上储存含有核酸的样品的方法，其中许多是商业可得的。