公开(公告)号：US07918981B2

公开(公告)日：2011-04-05

申请号：US11598848

申请日：2006-11-14

申请人： Gaoshan Jing ,  Jian Zhang ,  Jing Cheng

发明人： Gaoshan Jing ,  Jian Zhang ,  Jing Cheng

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： B03C5/005

摘要： This invention relates generally to the field of cell separation or isolation. In particular, the invention provides a method for separating cells, which method comprises: a) selectively staining cells to be separated with a dye so that there is a sufficient difference in a separable property of differentially stained cells; and b) separating said differentially stained cells via said separable property. Preferably, the separable property is dielectrophoretic property of the differentially stained cells and the differentially stained cells are separated or isolated via dielectrophoresis. Methods for separating various types of cells in blood samples are also provided. Centrifuge tubes useful in density gradient centrifugation and dielectrophoresis isolation devices useful for separating or isolating various types of cells are further provided.

摘要翻译： 本发明一般涉及细胞分离或分离领域。 特别地，本发明提供了一种分离细胞的方法，该方法包括：a）用染料选择性染色待分离的细胞，使得差异染色细胞的可分离性有足够的差异; 和b）通过所述可分离性质分离所述差异染色的细胞。 优选地，可分离性质是差异染色的细胞的介电电泳性质，并且通过介电电泳分离或分离差异染色的细胞。 还提供了用于分离血液样品中各种细胞的方法。 进一步提供了可用于分离或分离各种类型细胞的密度梯度离心和介电电泳分离装置的离心管。

公开(公告)号：US20070128686A1

公开(公告)日：2007-06-07

申请号：US11598848

申请日：2006-11-14

申请人： Gaoshan Jing ,  Jian Zhang ,  Jing Cheng

发明人： Gaoshan Jing ,  Jian Zhang ,  Jing Cheng

IPC分类号： C12Q1/04 ,  G01N33/48 ,  C12N5/04 ,  C12N5/06 ,  C12N1/20

CPC分类号： B03C5/005

摘要： This invention relates generally to the field of cell separation or isolation. In particular, the invention provides a method for separating cells, which method comprises: a) selectively staining cells to be separated with a dye so that there is a sufficient difference in a separable property of differentially stained cells; and b) separating said differentially stained cells via said separable property. Preferably, the separable property is dielectrophoretic property of the differentially stained cells and the differentially stained cells are separated or isolated via dielectrophoresis. Methods for separating various types of cells in blood samples are also provided. Centrifuge tubes useful in density gradient centrifugation and dielectrophoresis isolation devices useful for separating or isolating various types of cells are further provided.

摘要翻译： 本发明一般涉及细胞分离或分离领域。 特别地，本发明提供了一种分离细胞的方法，该方法包括：a）用染料选择性染色待分离的细胞，使得差异染色细胞的可分离性有足够的差异; 和b）通过所述可分离性质分离所述差异染色的细胞。 优选地，可分离性质是差异染色的细胞的介电电泳性质，并且通过介电电泳分离或分离差异染色的细胞。 还提供了用于分离血液样品中各种细胞的方法。 进一步提供了可用于分离或分离各种类型细胞的密度梯度离心和介电电泳分离装置的离心管。

公开(公告)号：US07153648B2

公开(公告)日：2006-12-26

申请号：US10103581

申请日：2002-03-20

申请人： Gaoshan Jing ,  Jian Zhang ,  Jing Cheng

发明人： Gaoshan Jing ,  Jian Zhang ,  Jing Cheng

IPC分类号： A01N1/02

CPC分类号： B03C5/005

摘要： This invention relates generally to the field of cell separation or isolation. In particular, the invention provides a method for separating cells, which method comprises: a) selectively staining cells to be separated with a dye so that there is a sufficient difference in a separable property of differentially stained cells; and b) separating said differentially stained cells via said separable property. Preferably, the separable property is dielectrophoretic property of the differentially stained cells and the differentially stained cells are separated or isolated via dielectrophoresis. Methods for separating various types of cells in blood samples are also provided. Centrifuge tubes useful in density gradient centrifugation and dielectrophoresis isolation devices useful for separating or isolating various types of cells are further provided.

摘要翻译： 本发明一般涉及细胞分离或分离领域。 特别地，本发明提供了一种分离细胞的方法，该方法包括：a）用染料选择性染色待分离的细胞，使得差异染色细胞的可分离性有充分差异; 和b）通过所述可分离性质分离所述差异染色的细胞。 优选地，可分离性质是差异染色的细胞的介电电泳性质，并且通过介电电泳分离或分离差异染色的细胞。 还提供了用于分离血液样品中各种细胞的方法。 进一步提供了可用于分离或分离各种类型细胞的密度梯度离心和介电电泳分离装置的离心管。