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公开(公告)号：US20140243232A1
公开(公告)日：2014-08-28
申请号：US14232572
申请日：2012-07-13
申请人： Gavin Meredith , Christopher Clouser , Tanya Sokolsky , Kimberly Mather , Kevin McKernan , Marie Callahan , Gary Bee
发明人： Gavin Meredith , Christopher Clouser , Tanya Sokolsky , Kimberly Mather , Kevin McKernan , Marie Callahan , Gary Bee
CPC分类号： C12Q1/6874 , C12Q1/6832 , C12Q2525/191 , C12Q2537/159 , C12Q2537/163 , C12Q2563/131
摘要： In some embodiments, the present teachings provide compositions, systems, methods and kits for reducing the complexity of nucleotide sequences in a nucleic acid sample comprising the steps: hybridizing a plurality of polynucleotide constructs to at least one blocker oligonucleotide and to at least one capture oligonucleotide, wherein the plurality of polynucleotide constructs include a plurality of polynucleotides each joined to at least one nucleic acid adaptor, wherein the at least one nucleic acid adaptor can hybridize to the at least one blocker oligonucleotide, and wherein the at least one capture oligonucleotide can hybridize to at least a portion of target polynucleotides that are a sub-population of the plurality of polynucleotides, so as to produce a capture duplex.
摘要翻译： 在一些实施方案中，本教导提供了用于降低核酸样品中核苷酸序列的复杂性的组合物，系统，方法和试剂盒，其包括以下步骤：将多个多核苷酸构建体与至少一个阻断寡核苷酸和至少一个捕获寡核苷酸杂交 其中所述多个多核苷酸构建体包括多个多核苷酸，其各自与至少一个核酸衔接子连接，其中所述至少一个核酸衔接子可以与所述至少一个阻断寡核苷酸杂交，并且其中所述至少一个捕获寡核苷酸可以杂交 至少一部分作为多个多核苷酸的亚群的靶多核苷酸，以产生捕获双链体。
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公开(公告)号：US20110237444A1
公开(公告)日：2011-09-29
申请号：US12950227
申请日：2010-11-19
CPC分类号： C12Q1/6888 , C12Q1/6827 , C12Q2600/154 , C12Q2523/125
摘要： The invention relates to sample analysis work flows for increasing the efficiency of experiments. Compositions and methods are described for selectively increase the abundance of methylated nucleic acid over non-methylated nucleic acid, followed by analysis of the nucleic acid to identify methylation sites.
摘要翻译： 本发明涉及用于提高实验效率的样品分析工作流程。 描述了组合物和方法，用于选择性地增加甲基化核酸在非甲基化核酸上的丰度，随后分析核酸以鉴定甲基化位点。
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