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公开(公告)号：US10041061B2
公开(公告)日：2018-08-07
申请号：US13876144
申请日：2011-09-23
摘要： The invention provides methods for extraction of fungal (e.g., yeast spp., filamentous fungal spp.) nucleic acid (e.g., DNA, RNA) from a sample (e.g., be human or veterinary clinical or research samples, agricultural samples, agricultural commodity samples, food products, or environmental samples). In some embodiments, the present invention provides enhanced nucleic acid extraction from samples comprising fungal cell(s) wherein enzymatic (e.g., lysostaphin treatment, lyticase treatment) sample treatment is performed in combination with mechanical (e.g., bead beating) sample treatment.
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公开(公告)号：US20130323815A1
公开(公告)日：2013-12-05
申请号：US13876144
申请日：2011-09-23
IPC分类号： C12N15/10
CPC分类号： C12N15/1003 , C12N1/06 , C12N1/066
摘要： The invention provides methods for extraction of fungal (e.g., yeast spp., filamentous fungal spp.) nucleic acid (e.g., DNA, RNA) from a sample (e.g., be human or veterinary clinical or research samples, agricultural samples, agricultural commodity samples, food products, or environmental samples). In some embodiments, the present invention provides enhanced nucleic acid extraction from samples comprising fungal cell(s) wherein enzymatic (e.g., lysostaphin treatment, lyticase treatment) sample treatment is performed in combination with mechanical (e.g., bead beating) sample treatment.
摘要翻译： 本发明提供从样品中提取真菌（例如，酵母菌，丝状真菌）核酸（例如，DNA，RNA）的方法（例如，人或兽医临床或研究样品，农业样品，农业商品样品 ，食品或环境样品）。 在一些实施方案中，本发明从包含真菌细胞的样品提供增强的核酸提取，其中酶促（例如，溶葡萄球菌素处理，溶解酶处理）样品处理与机械（例如，珠打浆）样品处理组合进行。
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公开(公告)号：US06936414B2
公开(公告)日：2005-08-30
申请号：US09470944
申请日：1999-12-22
申请人： Gerard Gundling
发明人： Gerard Gundling
CPC分类号： C12N15/1013
摘要： Provided herein is a method for separating nucleic acid from a test sample comprising the steps of contacting a test sample with a metal oxide support material and a binding buffer to form nucleic acid/metal oxide support material complexes, separating the complexes from the test sample; and eluting the nucleic acid from the metal oxide support material.
摘要翻译： 本文提供了一种从测试样品中分离核酸的方法，包括以下步骤：使测试样品与金属氧化物载体材料和结合缓冲液接触，以形成核酸/金属氧化物载体材料复合物，将复合物与测试样品分离; 并从金属氧化物载体材料中洗脱核酸。
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