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公开(公告)号：US20130323815A1
公开(公告)日：2013-12-05
申请号：US13876144
申请日：2011-09-23
IPC分类号： C12N15/10
CPC分类号： C12N15/1003 , C12N1/06 , C12N1/066
摘要： The invention provides methods for extraction of fungal (e.g., yeast spp., filamentous fungal spp.) nucleic acid (e.g., DNA, RNA) from a sample (e.g., be human or veterinary clinical or research samples, agricultural samples, agricultural commodity samples, food products, or environmental samples). In some embodiments, the present invention provides enhanced nucleic acid extraction from samples comprising fungal cell(s) wherein enzymatic (e.g., lysostaphin treatment, lyticase treatment) sample treatment is performed in combination with mechanical (e.g., bead beating) sample treatment.
摘要翻译： 本发明提供从样品中提取真菌（例如，酵母菌，丝状真菌）核酸（例如，DNA，RNA）的方法（例如，人或兽医临床或研究样品，农业样品，农业商品样品 ，食品或环境样品）。 在一些实施方案中，本发明从包含真菌细胞的样品提供增强的核酸提取，其中酶促（例如，溶葡萄球菌素处理，溶解酶处理）样品处理与机械（例如，珠打浆）样品处理组合进行。
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公开(公告)号：US10041061B2
公开(公告)日：2018-08-07
申请号：US13876144
申请日：2011-09-23
摘要： The invention provides methods for extraction of fungal (e.g., yeast spp., filamentous fungal spp.) nucleic acid (e.g., DNA, RNA) from a sample (e.g., be human or veterinary clinical or research samples, agricultural samples, agricultural commodity samples, food products, or environmental samples). In some embodiments, the present invention provides enhanced nucleic acid extraction from samples comprising fungal cell(s) wherein enzymatic (e.g., lysostaphin treatment, lyticase treatment) sample treatment is performed in combination with mechanical (e.g., bead beating) sample treatment.
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公开(公告)号：US20120164633A1
公开(公告)日：2012-06-28
申请号：US13337819
申请日：2011-12-27
申请人： Thomas Laffler
发明人： Thomas Laffler
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2563/159
摘要： The present invention provides systems, devices, methods, kits, and compositions for sorting and analysis of nucleic acid sequences using digital droplet PCR. In particular, provided herein are methods to convert complex samples into a plurality of simplified samples, and sequence analysis thereof.
摘要翻译： 本发明提供了使用数字液滴PCR对核酸序列进行分选和分析的系统，装置，方法，试剂盒和组合物。 特别地，本文提供了将复杂样品转化为多个简化样品的方法及其序列分析。
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公开(公告)号：US20130184446A1
公开(公告)日：2013-07-18
申请号：US13724527
申请日：2012-12-21
申请人： Herbert A. Marble , Thomas Laffler
发明人： Herbert A. Marble , Thomas Laffler
IPC分类号： C07H21/00
CPC分类号： B01L7/54 , B01L3/502784 , B01L7/525 , B01L2400/0415 , B01L2400/0433 , B01L2400/0445 , B01L2400/086 , C07H21/00 , C12P19/34 , C12Q1/686 , C12Q2527/15 , C12Q2563/159 , C12Q2565/629
摘要： The present disclosure provides systems, devices, and methods for purifying microorganism nucleic acid from a host sample, such as a whole blood sample from a human. In certain embodiments, devices and systems with multiple filters are employed and provide for the selective removal of blood cells and host nucleic acids from a sample in order to enrich for microorganism nucleic acid.
摘要翻译： 本公开提供了用于从宿主样品纯化微生物核酸的系统，装置和方法，例如来自人的全血样品。 在某些实施方案中，使用具有多个过滤器的装置和系统，并提供从样品中选择性除去血细胞和宿主核酸，以富集微生物核酸。
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公开(公告)号：US20130183717A1
公开(公告)日：2013-07-18
申请号：US13724503
申请日：2012-12-21
申请人： Herbert A. Marble , Thomas Laffler
发明人： Herbert A. Marble , Thomas Laffler
IPC分类号： C12P19/34
CPC分类号： B01L7/54 , B01L3/502784 , B01L7/525 , B01L2400/0415 , B01L2400/0433 , B01L2400/0445 , B01L2400/086 , C07H21/00 , C12P19/34 , C12Q1/686 , C12Q2527/15 , C12Q2563/159 , C12Q2565/629
摘要： Provided herein are systems, devices, and methods for generating thermal gradients in channels and uses thereof. In particular, provided herein are system, methods, and devices employing first and second thermal layers positioned around a channel in order to create a thermal gradient across a cross-section of the channel having, for example, a nucleic acid denaturation zone, a nucleic acid annealing zone, and a nucleic acid polymerization zone. Such devices find use in, for example, nucleic acid amplification procedures, including digital polymerase chain reaction (dPCR) to temperature cycle droplets for amplification of nucleic acid templates within the droplets.
摘要翻译： 本文提供了用于在通道中产生热梯度的系统，装置和方法及其用途。 特别地，本文提供了使用位于通道周围的第一和第二热层的系统，方法和装置，以便跨越具有例如核酸变性区的通道的横截面产生热梯度，核酸 酸退火区和核酸聚合区。 这样的装置可用于例如核酸扩增程序，包括数字聚合酶链反应（dPCR）到温度循环液滴，用于扩散液滴内的核酸模板。
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