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1.发明申请Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture 有权用于通过溶液捕获的质谱法快速纯化核酸以进行后续分析的方法公开(公告)号：US20050130196A1
公开(公告)日：2005-06-16
申请号：US10943344
申请日：2004-09-17
申请人： Steven Hofstadler , Lendell Cummins
发明人： Steven Hofstadler , Lendell Cummins
CPC分类号： C12Q1/6806 , C12N15/101 , C12N15/1013 , H01J49/165 , C12Q2563/155 , C12Q2563/143 , C12Q2565/627
摘要： The present invention provides a method for rapid solution capture purification of nucleic acids for subsequent analysis by electrospray mass spectrometry which is efficient and cost-effective relative to existing methods. The present invention also provides for kits useful for practicing rapid solution capture of nucleic acids so that purified samples are in condition for analysis by electrospray mass spectrometry.
摘要翻译： 本发明提供了一种用于快速溶液捕获纯化核酸的方法，用于随后通过电喷雾质谱法进行分析，其相对于现有方法是有效且成本有效的。 本发明还提供了用于实施核酸的快速溶液捕获的试剂盒，使得纯化的样品处于通过电喷雾质谱分析的条件下。
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公开(公告)号：US20130323815A1
公开(公告)日：2013-12-05
申请号：US13876144
申请日：2011-09-23
IPC分类号： C12N15/10
CPC分类号： C12N15/1003 , C12N1/06 , C12N1/066
摘要： The invention provides methods for extraction of fungal (e.g., yeast spp., filamentous fungal spp.) nucleic acid (e.g., DNA, RNA) from a sample (e.g., be human or veterinary clinical or research samples, agricultural samples, agricultural commodity samples, food products, or environmental samples). In some embodiments, the present invention provides enhanced nucleic acid extraction from samples comprising fungal cell(s) wherein enzymatic (e.g., lysostaphin treatment, lyticase treatment) sample treatment is performed in combination with mechanical (e.g., bead beating) sample treatment.
摘要翻译： 本发明提供从样品中提取真菌（例如，酵母菌，丝状真菌）核酸（例如，DNA，RNA）的方法（例如，人或兽医临床或研究样品，农业样品，农业商品样品 ，食品或环境样品）。 在一些实施方案中，本发明从包含真菌细胞的样品提供增强的核酸提取，其中酶促（例如，溶葡萄球菌素处理，溶解酶处理）样品处理与机械（例如，珠打浆）样品处理组合进行。
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公开(公告)号：US10041061B2
公开(公告)日：2018-08-07
申请号：US13876144
申请日：2011-09-23
摘要： The invention provides methods for extraction of fungal (e.g., yeast spp., filamentous fungal spp.) nucleic acid (e.g., DNA, RNA) from a sample (e.g., be human or veterinary clinical or research samples, agricultural samples, agricultural commodity samples, food products, or environmental samples). In some embodiments, the present invention provides enhanced nucleic acid extraction from samples comprising fungal cell(s) wherein enzymatic (e.g., lysostaphin treatment, lyticase treatment) sample treatment is performed in combination with mechanical (e.g., bead beating) sample treatment.
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4.发明申请Method for rapid identification of molecules with functional activity towards biomolecular targets 审中-公开快速鉴定具有生物分子靶标功能活性的分子的方法公开(公告)号：US20050095622A1
公开(公告)日：2005-05-05
申请号：US10922739
申请日：2004-08-20
IPC分类号： C07H21/02 , C12Q1/68 , G01N20060101 , G01N33/68
CPC分类号： C12Q1/68 , G01N33/6848 , C12Q2565/627
摘要： The present invention provides mass spectrometry-based methods for high throughput identification of molecules with functional activities such as nuclease, protease, reductase, kinase, phosphatase, or transferase activities towards biomolecular targets. These methods are useful for screening biological samples such as extracts, broths, lysates and natural product mixtures and provide valuable insights into biomolecular interactions of various biological ligands with biomolecular targets.
摘要翻译： 本发明提供基于质谱法的高通量鉴定具有功能活性如分子核酸酶，蛋白酶，还原酶，激酶，磷酸酶或转移酶活性的生物分子靶标的分子。 这些方法可用于筛选生物样品如提取物，肉汤，裂解物和天然产物混合物，并为各种生物配体与生物分子靶标的生物分子相互作用提供有价值的见解。
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5.发明申请METHOD FOR RAPID IDENTIFICATION OF MOLECULES WITH FUNCTIONAL ACTIVITY TOWARDS BIOMOLECULAR TARGETS 审中-公开具有功能活性的生物分子靶标快速鉴定分子的方法公开(公告)号：US20070264661A1
公开(公告)日：2007-11-15
申请号：US11774463
申请日：2007-07-06
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/573
CPC分类号： C12Q1/68 , G01N33/6848 , C12Q2565/627
摘要： The present invention provides mass spectrometry-based methods for high throughput identification of molecules with functional activities such as nuclease, protease, reductase, kinase, phosphatase, or transferase activities towards biomolecular targets. These methods are useful for screening biological samples such as extracts, broths, lysates and natural product mixtures and provide valuable insights into biomolecular interactions of various biological ligands with biomolecular targets.
摘要翻译： 本发明提供基于质谱法的高通量鉴定具有功能活性如分子核酸酶，蛋白酶，还原酶，激酶，磷酸酶或转移酶活性的生物分子靶标的分子。 这些方法可用于筛选生物样品如提取物，肉汤，裂解物和天然产物混合物，并为各种生物配体与生物分子靶标的生物分子相互作用提供有价值的见解。
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6.发明申请Methods for rapid purification of nucleic acids for subsquent analysis by mass spectrometery by solution capture 有权通过溶液捕获通过质谱法快速纯化核酸进行次级分析的方法公开(公告)号：US20050164215A1
公开(公告)日：2005-07-28
申请号：US10844938
申请日：2004-05-12
申请人： Steven Hofstadler , Lendell Cummins
发明人： Steven Hofstadler , Lendell Cummins
CPC分类号： C12N15/101 , C12Q1/6806 , C12Q2563/155 , C12Q2563/143 , C12Q2565/627
摘要： The present invention provides a method for rapid solution capture purification of nucleic acids for subsequent analysis by electrospray mass spectrometry which is efficient and cost-effective relative to existing methods. The present invention also provides for kits useful for practicing rapid solution capture of nucleic acids so that purified samples are in condition for analysis by electrospray mass spectrometry.
摘要翻译： 本发明提供了一种用于快速溶液捕获纯化核酸的方法，用于随后通过电喷雾质谱法进行分析，其相对于现有方法是有效且成本有效的。 本发明还提供了用于实施核酸的快速溶液捕获的试剂盒，使得纯化的样品处于用于通过电喷雾质谱分析的条件。
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