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公开(公告)号:US10288603B2
公开(公告)日:2019-05-14
申请号:US13995610
申请日:2012-01-13
申请人: Glauco R. Souza
发明人: Glauco R. Souza
IPC分类号: G01N33/50 , C12N13/00 , C12N11/00 , C12Q1/02 , B03C1/00 , B82Y25/00 , G01R33/12 , C12N5/07 , H01F1/44 , H01F1/08 , H01F1/113 , H01F1/06
摘要: Cells are grown in 3D culture and topological features obtained by photomicrography are correlated to cell viability and cell cell interactions.
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公开(公告)号:US08883471B2
公开(公告)日:2014-11-11
申请号:US13393651
申请日:2010-09-27
申请人: Glauco R. Souza
发明人: Glauco R. Souza
IPC分类号: C12Q1/02 , C12N13/00 , C12N11/00 , C12N5/00 , G01R33/12 , B03C1/015 , B03C1/30 , B82Y25/00 , G01N33/543 , G01N33/544 , H01F1/00 , H01F1/08 , H01F1/113 , H01F1/44 , C12Q1/24 , C12N1/02 , B82B3/00
CPC分类号: C12N5/0062 , B03C1/015 , B03C1/30 , B03C2201/26 , B82Y25/00 , C12N5/00 , C12N2513/00 , C12N2529/00 , C12N2531/00 , C12N2533/10 , C12N2533/32 , G01N33/54326 , G01N33/544 , G01R33/1269 , H01F1/0045 , H01F1/0063 , H01F1/083 , H01F1/113 , H01F1/445 , Y10S977/773 , Y10S977/89 , Y10S977/904 , Y10S977/923
摘要: A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.
摘要翻译: 包含正电荷和带负电荷的纳米颗粒的材料,其中所述纳米颗粒中的一个包含磁响应元件,与作为磁性纳米颗粒组件的长天然或合成分子或聚合物的载体分子组合。 当磁性纳米颗粒组件与细胞结合时,它将磁化这些细胞。 然后可以洗涤磁化的细胞以除去磁性纳米颗粒组件,并且磁化的细胞在磁场中操作。
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公开(公告)号:US20130280754A1
公开(公告)日:2013-10-24
申请号:US13995610
申请日:2012-01-13
申请人: Glauco R. Souza
发明人: Glauco R. Souza
IPC分类号: G01N33/50
CPC分类号: G01N33/5088 , G01N33/5082
摘要: Cells are grown in 3D culture and topological features obtained by photomicrography are correlated to cell viability and cell cell interactions.
摘要翻译: 细胞生长在3D培养中,通过显微照相获得的拓扑特征与细胞活力和细胞相互作用相关。
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4.
公开(公告)号:US20110286975A1
公开(公告)日:2011-11-24
申请号:US13070873
申请日:2011-03-24
申请人: Glauco R. Souza , Renata Pasqualini , Wadih Arap , Thomas Charles Killian , Robert M. Raphael , Daniel Joshua Stark
发明人: Glauco R. Souza , Renata Pasqualini , Wadih Arap , Thomas Charles Killian , Robert M. Raphael , Daniel Joshua Stark
CPC分类号: C12N13/00 , B82Y5/00 , C12M35/06 , C12N5/0062 , C12N2529/00 , C12N2533/10
摘要: Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.
摘要翻译: 讨论了一般在医学,细胞生物学,纳米技术和细胞培养中有用的系统和方法。 特别地,至少在一些实施例中,讨论了用于细胞和材料的磁性引导和图案化的系统和方法。 这些系统和方法的一些具体应用可以包括将细胞悬浮培养离开表面,制备和操纵悬浮细胞的图案,以及对表面上的细胞进行图案化培养。 具体地说,提出培养细胞的方法。 该方法可以包括提供多个单元,提供磁场,以及悬浮在磁场中的多个单元中的至少一些单元,其中多个单元包括磁性纳米颗粒。 该方法还可以包括将悬浮液维持足以允许细胞生长形成组件的时间。
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公开(公告)号:US20120171744A1
公开(公告)日:2012-07-05
申请号:US13393651
申请日:2010-09-27
申请人: Glauco R. Souza
发明人: Glauco R. Souza
CPC分类号: C12N5/0062 , B03C1/015 , B03C1/30 , B03C2201/26 , B82Y25/00 , C12N5/00 , C12N2513/00 , C12N2529/00 , C12N2531/00 , C12N2533/10 , C12N2533/32 , G01N33/54326 , G01N33/544 , G01R33/1269 , H01F1/0045 , H01F1/0063 , H01F1/083 , H01F1/113 , H01F1/445 , Y10S977/773 , Y10S977/89 , Y10S977/904 , Y10S977/923
摘要: A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.
摘要翻译: 包含正电荷和带负电荷的纳米颗粒的材料,其中所述纳米颗粒中的一个包含磁响应元件,与作为磁性纳米颗粒组件的长天然或合成分子或聚合物的载体分子组合。 当磁性纳米颗粒组件与细胞结合时,它将磁化这些细胞。 然后可以洗涤磁化的细胞以除去磁性纳米颗粒组件,并且磁化的细胞在磁场中操作。
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6.
公开(公告)号:US08815231B2
公开(公告)日:2014-08-26
申请号:US13070873
申请日:2011-03-24
申请人: Glauco R. Souza , Renata Pasqualini , Wadih Arap , Thomas Charles Killian , Robert M. Raphael , Daniel Joshua Stark
发明人: Glauco R. Souza , Renata Pasqualini , Wadih Arap , Thomas Charles Killian , Robert M. Raphael , Daniel Joshua Stark
IPC分类号: C12N5/00
CPC分类号: C12N13/00 , B82Y5/00 , C12M35/06 , C12N5/0062 , C12N2529/00 , C12N2533/10
摘要: Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.
摘要翻译: 讨论了一般在医学,细胞生物学,纳米技术和细胞培养中有用的系统和方法。 特别地,至少在一些实施例中,讨论了用于细胞和材料的磁性引导和图案化的系统和方法。 这些系统和方法的一些具体应用可以包括将细胞悬浮培养离开表面,制备和操纵悬浮细胞的图案,以及对表面上的细胞进行图案化培养。 具体地说,提出培养细胞的方法。 该方法可以包括提供多个单元,提供磁场,以及悬浮在磁场中的多个单元中的至少一些单元,其中多个单元包括磁性纳米颗粒。 该方法还可以包括将悬浮液维持足以允许细胞生长形成组件的时间。
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